{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T21:22:39Z","timestamp":1790198559105,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,3,30]],"date-time":"2020-03-30T00:00:00Z","timestamp":1585526400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2019M662270"],"award-info":[{"award-number":["2019M662270"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aimed to evaluate the effects of dietary guanidine acetic acid (GAA) supplementation on growth performance, carcass traits and the expression of muscle growth\u2010related genes in finishing pigs. A total of 128 <jats:bold>(<\/jats:bold>81.03\u00a0\u00b1\u00a01.09\u00a0kg body weight) crossbred pigs (Duroc\u00a0\u00d7\u00a0Landrace \u00d7Yorkshire) were blocked by body weight and allotted to 16 pens (eight pigs per pen), and pens were randomly assigned within blocks to one of five dietary treatments, with a basal diet (control group) or a basal diet supplemented with 0.03%, 0.06% and 0.09% GAA respectively. During the 60\u2010day trial, GAA increased the average dairy gain (ADG) and average daily feed intake (ADFI) (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). The back fat thickness of pigs fed 0.06% GAA was lower than other groups (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). Pigs fed 0.06% GAA had improved lean meat percentage, loin muscle area, shear force and cross\u2010sectional area of muscle fibre in comparison with control group (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). The drop loss and the muscle fibre density in pigs fed 0.06% GAA were lower than control (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). In addition, dietary GAA enhanced the expression of myosin heavy chain gene (MYH4), myogenic determination (Myod) and myogenic factor 5 (Myf5) in longissimus dorsi and carnitine palmitoyltransferase\u20101(CPT\u20101) in liver (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). Meanwhile, GAA decreased the expression of Myostatin in longissimus dorsi and fatty acid synthase (FAS) in liver (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). In conclusion, our results showed that appropriate dietary GAA supplementation (0.06%) promotes skeletal muscle development through changing myogenic gene expression and myofibre characteristics.<\/jats:p>","DOI":"10.1111\/jpn.13351","type":"journal-article","created":{"date-parts":[[2020,3,30]],"date-time":"2020-03-30T09:39:10Z","timestamp":1585561150000},"page":"1875-1883","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Dietary guanidinoacetic acid improves the growth performance and skeletal muscle development of finishing pigs through changing myogenic gene expression and myofibre characteristics"],"prefix":"10.1111","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3965-8702","authenticated-orcid":false,"given":"Yafei","family":"Lu","sequence":"first","affiliation":[{"name":"Jiangxi Province Key Laboratory of Animal Nutrition Jiangxi Agricultural University Nanchang China"},{"name":"Jiangxi 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