{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:48:40Z","timestamp":1761709720943,"version":"3.40.5"},"reference-count":58,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,4,25]],"date-time":"2020-04-25T00:00:00Z","timestamp":1587772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["1S36216N"],"award-info":[{"award-number":["1S36216N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Phenolic compounds in pig diets, originating either from feed ingredients or additives, may occur as glycosides, that is conjugated to sugar moieties. Upon ingestion, their bioavailability and functionality depend on hydrolysis of the glycosidic bond by endogenous or microbial glycosidases. Hence, it is essential to map the glycosidase activities towards phenolic glycosides present along gut. Therefore, the activity of three key glycosidases, that is \u03b1\u2010glucosidase (\u03b1GLU), \u03b2\u2010glucosidase (\u03b2GLU) and \u03b2\u2010galactosidase (\u03b2GAL), was quantified in small intestinal mucosa and digesta of piglets at different gastrointestinal sites (stomach, three parts of small intestine, caecum and colon) and at different ages around weaning (10\u00a0days before and 0, 2, 5, 14 and 28\u00a0days after weaning). Activity assays were performed with <jats:italic>p<\/jats:italic>\u2010nitrophenyl glycosides at neutral pH. The \u03b1GLU activities in mucosa and digesta were low (overall means 1.4 and 60\u00a0U respectively) as compared to \u03b2GLU (15.2 and 199\u00a0U) and \u03b2GAL (23.4 and 298\u00a0U; <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.001). Moreover, \u03b1GLU activity in mucosa was unaffected by age. Conversely, \u03b2GLU and \u03b2GAL activities dropped significantly after weaning. Minimal levels, ranging between 18% and 54% of the pre\u2010weaning values, were reached at 5\u00a0days post\u2010weaning. Similarly, in small intestinal digesta, reductions from 60% up to 90% were observed for the three enzyme activities on day five post\u2010weaning as compared to pre\u2010weaning levels. In caecal contents, activities were lowest at 14\u00a0days post\u2010weaning, while in stomach and colon no clear weaning\u2010induced effects were observed. Our data suggest that weaning affects the glycosidase activity in mucosa (mainly endogenous origin) and digesta (primarily bacterial origin) with the most pronounced effects occurring 5\u00a0days post\u2010weaning. Moreover, differences in activities exist between different glycosidases and between gut locations. These insights can facilitate the prediction of the fate of existing and newly synthetized glycosides after oral ingestion in piglets.<\/jats:p>","DOI":"10.1111\/jpn.13368","type":"journal-article","created":{"date-parts":[[2020,4,25]],"date-time":"2020-04-25T08:53:51Z","timestamp":1587804831000},"page":"1432-1443","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Weaning affects the glycosidase activity towards phenolic glycosides in the gut of piglets"],"prefix":"10.1111","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9597-9404","authenticated-orcid":false,"given":"No\u00e9mie","family":"Van Noten","sequence":"first","affiliation":[{"name":"Department of Animal Sciences and Aquatic Ecology Ghent University Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elout","family":"Van Liefferinge","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences and Aquatic Ecology Ghent University Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeroen","family":"Degroote","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences and Aquatic Ecology Ghent University Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefaan","family":"De Smet","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences and Aquatic Ecology Ghent University Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom","family":"Desmet","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Ghent University Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9577-0045","authenticated-orcid":false,"given":"Joris","family":"Michiels","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences and Aquatic Ecology Ghent University Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,4,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1079\/BJN20041123"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000300539"},{"issue":"1","key":"e_1_2_8_4_1","first-page":"51","article-title":"The development of the digestive enzyme system of the pig during its pre\u2010weaning phase of growth: B. 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