{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T23:22:38Z","timestamp":1768260158070,"version":"3.49.0"},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,5,15]],"date-time":"2020-05-15T00:00:00Z","timestamp":1589500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012453","name":"China Agricultural Research System","doi-asserted-by":"crossref","award":["CARS-42-G15"],"award-info":[{"award-number":["CARS-42-G15"]}],"id":[{"id":"10.13039\/501100012453","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The agricultral science and technology innovation program","award":["ASTIP 2014"],"award-info":[{"award-number":["ASTIP 2014"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this experiment was to study the effects of dietary addition of high\u2010dose <jats:italic>Moringa<\/jats:italic> extract (10\u00a0g\/kg Moringa extract) on laying performance, haematological parameters, organ index and organ histopathology of Hailan brown laying hens in order to determine the safety limit of <jats:italic>Moringa<\/jats:italic> extract in laying hens diet. 270 Hailan brown commercial laying hens with similar body weight were randomly divided into three treatments, 6 replicates per treatment and 15 chickens per replicate. Corn\u2013soybean meal\u2010based diet was used in the experiment, and 0, 0.75 and 10\u00a0g\/kg extracts of <jats:italic>Moringa oleifera<\/jats:italic> were added to the basic diet in the experimental group. The experiment lasted 24\u00a0weeks. The results showed that the treatment group given 10\u00a0g\/kg of dietary <jats:italic>Moringa<\/jats:italic> extract had no significant difference in egg\u2010laying performance from the control group (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05). Adding 0.75\u00a0g\/kg <jats:italic>Moringa<\/jats:italic> extract had no significant effect on the production performance during the early stage of the experiment (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05), but during the later stage, F\/E was significantly lower than in the control group (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05), and no significantly difference compared with 10\u00a0g\/kg treatment group (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05). The addition of <jats:italic>Moringa oleifera<\/jats:italic> extract had no significant effect on serum biochemical indices of laying hens except ALB and UREA (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05). The addition of <jats:italic>Moringa oleifera<\/jats:italic> extract had no significant effect on blood routine indexes of laying hens except for PLT, MON and HCT (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05). The digestive and immune organ indexes of laying hens at the later stage of the experiment (24\u00a0weeks) were not significantly affected by the addition of <jats:italic>Moringa<\/jats:italic> extract (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05) except ceca. There were no obvious abnormalities in histological structure. On the whole, we find it is safe to add <jats:italic>Moringa oleifera<\/jats:italic> extract to the diet of laying hens at a dosage of less than 10\u00a0g\/kg.<\/jats:p>","DOI":"10.1111\/jpn.13369","type":"journal-article","created":{"date-parts":[[2020,5,16]],"date-time":"2020-05-16T12:31:03Z","timestamp":1589632263000},"page":"1375-1383","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Tolerance evaluation of <i>Moringa oleifera<\/i> extract to Hailan brown laying hens"],"prefix":"10.1111","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2789-2670","authenticated-orcid":false,"given":"Zhi\u2010min","family":"Chen","sequence":"first","affiliation":[{"name":"Feed Research Institute Chinese Academy of Agricultural Sciences Key Laboratory of Feed Biotechnology of Agricultural Ministry National Engineering Research Center of Biological Feed Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen\u2010huan","family":"Chang","sequence":"additional","affiliation":[{"name":"Feed Research Institute Chinese Academy of Agricultural Sciences Key Laboratory of Feed Biotechnology of Agricultural Ministry National Engineering Research Center of Biological Feed Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ai\u2010juan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Feed Research Institute Chinese Academy of Agricultural Sciences Key Laboratory of Feed Biotechnology of Agricultural Ministry National Engineering Research Center of Biological Feed Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui\u2010yi","family":"Cai","sequence":"additional","affiliation":[{"name":"Feed Research Institute Chinese Academy of Agricultural Sciences Key Laboratory of Feed Biotechnology of Agricultural Ministry National Engineering Research Center of Biological Feed Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo\u2010hua","family":"Liu","sequence":"additional","affiliation":[{"name":"Feed Research Institute Chinese Academy of Agricultural Sciences Key Laboratory of Feed Biotechnology of Agricultural Ministry National Engineering Research Center of Biological Feed Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,5,15]]},"reference":[{"issue":"2","key":"e_1_2_9_2_1","first-page":"163","article-title":"Nutritional effects of dietary inclusion of Leucaena \n                  leucocephala and Moringa \n                  oleifera leaf meal on Rhode Island Red hens performance","volume":"45","author":"Abou\u2010Elezz F. M. K.","year":"2011","journal-title":"Cuban Journal of Agricultural Science"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-011-0037-5"},{"key":"e_1_2_9_4_1","first-page":"1","article-title":"Effect of various levels of Moringa leaf meal on the egg quality of Isa Brown Breed of layers","volume":"14","author":"Ebenebe C. I.","year":"2013","journal-title":"Advances in Life Science and Technology"},{"key":"e_1_2_9_5_1","first-page":"237","volume-title":"The clinical chemistry of laboratory animals","author":"Hoffmann W. E.","year":"1989"},{"key":"e_1_2_9_6_1","first-page":"3445","article-title":"Effect of dynamic subdivision of dietary nutrition supply on broiler performance, meat quality, nitrogen and phosphorus metabolism rate and serum biochemical indexes","volume":"28","author":"Huang X. Y.","year":"2016","journal-title":"Journal of Animal Nutrition"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.2005.42"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpn.12634"},{"issue":"2","key":"e_1_2_9_9_1","first-page":"223","article-title":"Effects of Moringa \n                  oleifera leaf meal on the quality, antioxidant and cholesterol content of eggs","volume":"10","author":"Lala A. O.","year":"2012","journal-title":"Journal of Agriculture, Forestry and the Social Sciences"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/1828051X.2016.1249967"},{"key":"e_1_2_9_11_1","first-page":"127","article-title":"Anti\u2010inflammatory effect of Moringa \n                  oleifera Lam. seeds on acetic acid\u2010induced acute colitis in rats","volume":"4","author":"Minaiyan M.","year":"2014","journal-title":"Avicenna Journal of Phytomedicine"},{"issue":"4","key":"e_1_2_9_12_1","first-page":"1","article-title":"Moringa \n                  oleifera leaf meal as a hypocholesterolemic agent in laying hen diets","volume":"22","author":"Olugbemi T. S.","year":"2010","journal-title":"Bone"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apcbee.2014.03.051"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3382\/japr.2013-00920"},{"key":"e_1_2_9_15_1","first-page":"8","article-title":"Effects of five Chinese herbal medicines on blood biochemical indexes of broilers","volume":"35","author":"Wan J. Y.","year":"2003","journal-title":"Animal Husbandry and Veterinary Medicine"},{"key":"e_1_2_9_16_1","first-page":"10","article-title":"Effect of horseradish leaf powder on performance, egg quality and serum biochemical index of laying hens","volume":"36","author":"Xi Q. Y.","year":"2015","journal-title":"Feed Industry"},{"key":"e_1_2_9_17_1","first-page":"35","article-title":"The effect of long\u2010term toxicity experiment of 24 toxic traditional Chinese medicines on organ index of rats","volume":"12","author":"Xiang L. H.","year":"2006","journal-title":"Chinese Journal of Basic Medicine of Traditional Chinese Medicine"},{"key":"e_1_2_9_18_1","volume-title":"Study on the effect and mechanism of Moringa oleracea on intestinal growth in mice","author":"Xu A.","year":"2016"},{"key":"e_1_2_9_19_1","first-page":"9","article-title":"Study on the regulation of nonspecific immune function of broiler by Naxi peptide","volume":"12","author":"Zhang D. L.","year":"2007","journal-title":"Feed Expo: Technical Edition"}],"container-title":["Journal of Animal Physiology and Animal Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjpn.13369","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpn.13369","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jpn.13369","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpn.13369","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T06:22:01Z","timestamp":1693894921000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpn.13369"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,15]]},"references-count":18,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["10.1111\/jpn.13369"],"URL":"https:\/\/doi.org\/10.1111\/jpn.13369","archive":["Portico"],"relation":{},"ISSN":["0931-2439","1439-0396"],"issn-type":[{"value":"0931-2439","type":"print"},{"value":"1439-0396","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,15]]},"assertion":[{"value":"2019-10-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}