{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:39:04Z","timestamp":1747186744266,"version":"3.40.5"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,6,8]],"date-time":"2020-06-08T00:00:00Z","timestamp":1591574400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Semen chilling has some harmful effects on sperm characteristics. Modification of extenders by using conventional and non\u2010conventional chilling substances can affect sperm characteristics and fertility. One of the important substances using to chilling human blood cells is citric phosphate dextrose (CPD); therefore, it can be used to store ram semen. This study aimed to assay the ability of CPD instead of egg yolk (EGD) and soybean lecithin (SLD)\u2010based extenders to store ram semen at 5\u00b0C. This trial was carried on pooling semen samples that were collected from five rams using a standard artificial vagina and an oestrous restrained ewe. Immediately after semen collection, semen was divided into four portions. The EGD, SLD and citric phosphate dextrose with an antibiotic (CPDA) and without antibiotic (CPD) were extender in the first, second, third and fourth portions of semen, respectively. Sperm characteristics were evaluated after dilution and cooling up to 7\u00a0days. Also, conception rate and fertility potential were assayed by 48 ewes (12 each) after 2\u00a0days of store ram semen extenders. Data clearly indicated that higher (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05) sperm characteristics in EGD, SLD and CPDA than CPD at 0\u00a0day of storage. Whilst, sperm characteristics from 1\u00a0day up to 7\u00a0days of storage were higher (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) in EGD, SLD, CPDA extenders than CPD. Moreover amongst extender types, the advancing of chilling days reduced sperm characteristics. Also, the results showed an acceptable conception rate and the fertility rate of dilution ram semen, which stored 2\u00a0days in EGD, SLD and CPDA than CPD. Using of CPD substances with antibiotic in chilling extension ram semen has benefits as well as EGD\u2010 and SLD\u2010based extenders. The future work should be conducted to validate these results in frozen ram semen.<\/jats:p>","DOI":"10.1111\/jpn.13389","type":"journal-article","created":{"date-parts":[[2020,6,8]],"date-time":"2020-06-08T13:25:26Z","timestamp":1591622726000},"page":"1620-1627","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The possibility of using citric phosphate dextrose in chilling ram semen instead of egg yolk and soybean lecithin to improve fertility"],"prefix":"10.1111","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9424-2523","authenticated-orcid":false,"given":"Mahmoud Yassin","family":"Mohamed","sequence":"first","affiliation":[{"name":"Animal Physiology Division Animal Production Research Institute (APRI) Agricultural Research Center (ARC) Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tarek M. M.","family":"Mahdy","sequence":"additional","affiliation":[{"name":"Animal Physiology Division Animal Production Research Institute (APRI) Agricultural Research Center (ARC) Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ezz I.","family":"Khalifa","sequence":"additional","affiliation":[{"name":"Animal Physiology Division Animal Production Research Institute (APRI) Agricultural Research Center (ARC) Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,6,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpn.13049"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpn.13303"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0223977"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.20546\/ijcmas.2019.801.290"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2016.06.009"},{"issue":"2","key":"e_1_2_9_7_1","first-page":"6637","article-title":"Bacterial contaminants and antimicrobial susceptibility profile of boar semen in southern Brazil Studs","volume":"23","author":"Bennemann P. 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