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One hundred and forty\u2010four pigs were assigned to (T1) 21% standard CP diet (SCP); (T2) SCP\u00a0+\u00a0ZnO (SCP ZnO); (T3) SCP\u00a0+\u00a0laminarin (SCP LAM); (T4) 18% low CP diet (LCP); (T5) LCP\u00a0+\u00a0ZnO (LCP ZnO); and (T6) LCP\u00a0+\u00a0laminarin (LCP LAM; <jats:italic>n<\/jats:italic>\u00a0=\u00a08 replicates\/treatment). The LCP diet had no effect on FS (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05), it increased two measures of alpha diversity, reduced Bacteroidetes and increased <jats:italic>Enterobacteriaceae<\/jats:italic> and <jats:italic>Helicobacteraceae<\/jats:italic> in the colon relative to the SCP diet (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). ZnO supplementation reduced FS and increased <jats:italic>Ruminococcaceae<\/jats:italic> compared with unsupplemented pigs (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). ZnO supplementation increased the genera <jats:italic>Frisingicoccus<\/jats:italic> (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.001), <jats:italic>Lachnoclostridium<\/jats:italic> (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) and <jats:italic>Peptoclostridium<\/jats:italic> (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) in the colon and reduced total caecal volatile fatty acids (VFA) concentrations compared with the unsupplemented and laminarin\u2010supplemented pigs. Laminarin supplementation reduced FS compared with unsupplemented pigs but had no major effect on the microbiota compared with the unsupplemented pigs. There were CP concentration\u00a0\u00d7\u00a0additive interactions on both Firmicutes and Proteobacteria. Firmicutes were increased in the LCP ZnO group compared with the LCP group, but there was no difference between the SCP groups. Proteobacteria were reduced in the LCP ZnO group compared with the LCP and LCP LAM groups (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05), but there was no difference between the SCP groups. In conclusion, reducing CP did not improve FS; it increased the relative abundance of <jats:italic>Enterobacteriaceae<\/jats:italic>; however, it also increased bacterial diversity. Supplementation with ZnO and laminarin improved FS, although all groups had scores within the healthy range. ZnO altered the large intestinal microbiota and VFA concentrations; however, laminarin did not enhance these parameters, suggesting these compounds have differing modes of action.<\/jats:p>","DOI":"10.1111\/jpn.13428","type":"journal-article","created":{"date-parts":[[2020,8,7]],"date-time":"2020-08-07T05:04:19Z","timestamp":1596776659000},"page":"1471-1483","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Effects of reducing dietary crude protein concentration and supplementation with laminarin or zinc oxide on the faecal scores and colonic microbiota in newly weaned pigs"],"prefix":"10.1111","volume":"104","author":[{"given":"Ruth","family":"Rattigan","sequence":"first","affiliation":[{"name":"School of Agriculture and Food Science University College Dublin Dublin 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