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L\u2010[<jats:sup>15<\/jats:sup>N] threonine was used to evaluate its incorporation rate and distribution among broiler tissues in different ages. Seventy\u2010two male broiler chickens were distributed into three different phases: starter (4 to 9\u00a0days old), grower (18 to 23\u00a0days old) and finisher phase (32 to 37\u00a0days old). L\u2010[<jats:sup>15<\/jats:sup>N] threonine was added on balanced diets, and birds were fed for five days in each phase. Enriched samples of breast muscle, feathers, liver, jejunum and plasma were collected at 0, 6, 12, 24, 48, 72, 96 and 120\u00a0hr after fed birds with the tracer in each phase. In the tissues were analysed dry matter, nitrogen and stable nitrogen. The <jats:sup>15<\/jats:sup>N isotope abundance according to the time was fitted into exponential or linear equations using a same intercept. The ratio of the steepness or slope coefficients was determined to compare the L\u2010[<jats:sup>15<\/jats:sup>N] threonine incorporation according to the age. In addition, L\u2010[<jats:sup>15<\/jats:sup>N] threonine mass balances were performed to assess the L\u2010[<jats:sup>15<\/jats:sup>N] threonine distribution among the evaluated tissues. Except for feathers, the L\u2010[<jats:sup>15<\/jats:sup>N] threonine incorporation rate decreased with ageing. Taking into account the L\u2010[<jats:sup>15<\/jats:sup>N] threonine distribution in the tissues, only in the jejunum was not observed an increase as the broiler grew. The L\u2010[<jats:sup>15<\/jats:sup>N] incorporation varied in each tissue and according to the age of the broiler chickens. These outcomes could be useful to comprehend changes in amino acid requirements tissue\u2010specific according to age.<\/jats:p>","DOI":"10.1111\/jpn.13478","type":"journal-article","created":{"date-parts":[[2021,1,10]],"date-time":"2021-01-10T17:46:00Z","timestamp":1610300760000},"page":"960-964","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Threonine incorporation in tissues of growing broiler chickens using L\u2010[<sup>15<\/sup>N] threonine"],"prefix":"10.1111","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8106-023X","authenticated-orcid":false,"given":"Rafael Massami","family":"Suzuki","sequence":"first","affiliation":[{"name":"School of Agriculture and Veterinarian Science S\u00e3o Paulo State University Jaboticabal Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matheus de Paula","family":"Reis","sequence":"additional","affiliation":[{"name":"School of Agriculture and Veterinarian Science S\u00e3o Paulo State University Jaboticabal Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana C\u00e9lia","family":"Denadai","sequence":"additional","affiliation":[{"name":"Stable Isotope Center \u201cProf. Carlos Ducatti\u201d S\u00e3o Paulo State University Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7803-6746","authenticated-orcid":false,"given":"Let\u00edcia Graziele","family":"Pacheco","sequence":"additional","affiliation":[{"name":"School of Agriculture and Veterinarian Science S\u00e3o Paulo State University Jaboticabal Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina Cardoso Nagib","family":"Nascimento","sequence":"additional","affiliation":[{"name":"School of Agriculture and Veterinarian Science S\u00e3o Paulo State University Jaboticabal Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5707-4113","authenticated-orcid":false,"given":"Nilva Kazue","family":"Sakomura","sequence":"additional","affiliation":[{"name":"School of Agriculture and Veterinarian Science S\u00e3o Paulo State University Jaboticabal Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2017.06.033"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1877-1823(09)70010-4"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1974.36.6.693"},{"volume-title":"Measuring model fit","year":"2015","author":"Kenny D. 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