{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T21:56:19Z","timestamp":1789422979088,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,3,19]],"date-time":"2021-03-19T00:00:00Z","timestamp":1616112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31760659"],"award-info":[{"award-number":["31760659"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31460582"],"award-info":[{"award-number":["31460582"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recent studies have shown elongase of very\u2010long\u2010chain fatty acids 6 (ELOVL6) is a vital protein for endogenous synthesis of saturated and monounsaturated long\u2010chain fatty acids in some mammals. Nevertheless, its role in lipid synthesis in buffalo mammary gland is still unclear. In this work, the full\u2010length coding sequence (CDS) of <jats:italic>ELOVL6<\/jats:italic> was cloned and identified from buffalo mammary gland. As a result, the CDS of this gene is 795\u00a0bp, which encodes a polypeptide of 264 amino acid residues. The buffalo ELOVL6 contains an ELO domain which belongs to the ELO superfamily. Among the 10 tissues of buffalo in peak lactation detected by RT\u2010qPCR, the expression level of ELOVL6 was the highest in the brain, followed by the spleen, and then decreased in the mammary gland, muscle, kidney, heart, liver, rumen, intestine and lung. However, only the expression in the brain and spleen was statistically different from that in other tissues (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). Compared with that of the dry\u2010off period, the mRNA abundance of <jats:italic>ELOVL6<\/jats:italic> in the mammary gland was significantly increased in peak lactation. The experiments based on lentivirus transfection in buffalo mammary epithelial cells (BuMECs) displayed that the overexpression of <jats:italic>ELOVL6<\/jats:italic> markedly promoted the expression of <jats:italic>INSIG1<\/jats:italic>, <jats:italic>INSIG2<\/jats:italic>, <jats:italic>SREBP<\/jats:italic>, <jats:italic>PPARG<\/jats:italic>, <jats:italic>FASN<\/jats:italic>, <jats:italic>GPAM<\/jats:italic>, <jats:italic>DGAT2<\/jats:italic> and <jats:italic>APGAT6<\/jats:italic> genes, and the knockdown of <jats:italic>ELOVL6<\/jats:italic> significantly decreased the mRNA abundance of <jats:italic>INSIG2<\/jats:italic>, <jats:italic>SREBP<\/jats:italic>, <jats:italic>FASN<\/jats:italic>, <jats:italic>SCD<\/jats:italic>, <jats:italic>GPAM<\/jats:italic>, <jats:italic>APGAT6<\/jats:italic> and <jats:italic>TIP47<\/jats:italic> genes. In addition, the increase or decrease of <jats:italic>ELOVL6<\/jats:italic> expression level also caused the corresponding change of total triglyceride content in the BuMECs. The results here suggest that the ELOVL6 can catalyse the synthesis of long\u2010chain fatty acids in the BuMECs, and it can indirectly affect the expression of genes related to milk fat synthesis through its catalytic products to promote the lipid biosynthesis of BuMECs.<\/jats:p>","DOI":"10.1111\/jpn.13536","type":"journal-article","created":{"date-parts":[[2021,3,20]],"date-time":"2021-03-20T04:31:29Z","timestamp":1616214689000},"page":"1-11","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Elongase of very long chain fatty acids 6 (ELOVL6) promotes lipid synthesis in buffalo mammary epithelial cells"],"prefix":"10.1111","volume":"106","author":[{"given":"Xinyang","family":"Fan","sequence":"first","affiliation":[{"name":"Faculty of Animal Science and Technology Yunnan Agricultural University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7166-8428","authenticated-orcid":false,"given":"Wei","family":"Zhu","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Technology Yunnan Agricultural University  Kunming China"},{"name":"College of Veterinary Medicine Yunnan Agricultural University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihua","family":"Qiu","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Technology Yunnan Agricultural University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangle","family":"Zhang","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Technology Yunnan Agricultural University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongyun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Technology Yunnan Agricultural University  Kunming China"},{"name":"Teaching Demonstration Center of the Basic Experiments of Agricultural Majors Yunnan Agricultural University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2127-0379","authenticated-orcid":false,"given":"Yongwang","family":"Miao","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Technology Yunnan Agricultural University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,3,19]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(05)73037-X"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2017-12789"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-366"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0175777"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12711-015-0111-y"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022029920000710"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(96)76398-1"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00544.2010"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M004747"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2009.12.002"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731108003133"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.28667"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2007-1850"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.01.075"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msw054"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1042\/0264-6021:3500765"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7827(03)00040-7"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1662"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)30465-X"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf302757j"},{"issue":"8","key":"e_1_2_11_22_1","first-page":"17","article-title":"Central role of the PPAR\u03b3 gene network in coordinating beef cattle intramuscular adipogenesis in response to weaning age and nutrition","volume":"8","author":"Mois\u00e1 S. 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