{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T07:36:39Z","timestamp":1781768199014,"version":"3.54.5"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,7,22]],"date-time":"2021-07-22T00:00:00Z","timestamp":1626912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We previously reported a study on 288 broiler (<jats:italic>Gallus gallus)<\/jats:italic> chicks who received caffeine in water between days 3 and 42, at levels of 0, 6.25, 12.5, 25, 50 and 100\u00a0mg\/kg body weight (BW)\/day. In the previous report, we found that caffeine caused pulmonary hypertension (PH)\u2010associated mortality in a significant minority (20%\u201330%) of birds, including right ventricular hypertrophy and ascites. We have also shown a significant upregulation of the serotonin transporter (SERT), troponin T2, adenosine A<jats:sub>1<\/jats:sub> receptor (ADORA1) and phosphodiesterase 5A (PDE5) in chicken suffering from PH. Here, we examine the resistant (survived) chicks from the first study that had not died due to acute heart failure and did not have clinical signs of pulmonary hypertension. Our goal was to determine whether birds who lacked overt signs of disease had subclinical manifestations, including similar changes in gene expression, growth rates and altered systemic haemodynamics. We found that growth was significantly increased by caffeine consumption (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.01) at low doses; however, dosage over 50\u00a0mg\/BW\/d had remarkable adverse effects on growth (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.01). Blood pressure, troponin T2 and PDE5 gene expression were not significantly altered by caffeine administration (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a00.05). However, SERT gene expression linearly increased with increasing caffeine dosage (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.01). The impact of caffeine on ADORA1 gene expression was dose dependent and nonlinear. In conclusion, despite the significant effects of caffeine on birds\u2019 growth, no significant negative effects of caffeine were observed on the cardiovascular function of resistant chickens. This work provides valuable information for further study on different dosage of caffeine in an animal model.<\/jats:p>","DOI":"10.1111\/jpn.13608","type":"journal-article","created":{"date-parts":[[2021,7,22]],"date-time":"2021-07-22T10:35:58Z","timestamp":1626950158000},"page":"566-574","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Impacts of caffeine on resistant chicken's performance and cardiovascular gene expression"],"prefix":"10.1111","volume":"106","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0925-240X","authenticated-orcid":false,"given":"Mohammad","family":"Kamely","sequence":"first","affiliation":[{"name":"Department of Poultry Science Faculty of Agriculture Tarbiat Modares University  Tehran Iran"},{"name":"Division Animal and Human Health Engineering Faculty of Bioscience Engineering University of KU Leuven  Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Amir","family":"Karimi Torshizi","sequence":"additional","affiliation":[{"name":"Department of Poultry Science Faculty of Agriculture Tarbiat Modares University  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"West","sequence":"additional","affiliation":[{"name":"Division of Allergy, Pulmonary, and Critical Care Medicine Department of Medicine Vanderbilt University Medical Center  Nashville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theo","family":"Niewold","sequence":"additional","affiliation":[{"name":"Niewold Consultancy  Bierbeek Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,7,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0031-1284434"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/00071660600611102"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1503\/cmaj\/051291"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0087547"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ntt.2011.08.010"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-200039020-00004"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11906-013-0377-9"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature17629"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.4081\/cardiogenetics.2016.6306"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2004.03.016"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2014.10.003"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1900\/RDS.2011.8.245"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10741-015-9522-7"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/dds137"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1515\/jbcpp-2016-0090"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3841.2010.01561.x"},{"key":"e_1_2_8_18_1","article-title":"Critical review of dietary caffeine and blood pressure: A relationship that should be taken more seriously","volume":"63","author":"James J. 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