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The cloned cDNA sequence was 2202\u00a0bp at full length, encoding a 733 amino acid peptide with a molecular weight of 81.9\u00a0kDa and a theoretical pI of 8.92. Bioinformatics analysis showed that the deduced peptide sequence possessed all the characteristic features of PepT2. The expression of PepT2 in the kidney and lung was significantly higher than that observed in the ileum, duodenum, jejunum, spleen, liver, heart and stomach. Functional characterization by heterologous expression in Chinese hamster ovary cells showed that the uptake of \u03b2\u2010Ala\u2010Lys\u2010N\u20107\u2010amino\u20104\u2010methylcoumarin\u20103\u2010acetic acid (\u03b2\u2010Ala\u2010Lys\u2010AMCA) by donkey PepT2\u2010Chinese hamster ovary cells was dependent on time, pH and substrate concentration, with a low Km value of 91.51\u00a0\u00b1\u00a014.14\u00a0\u03bcM and a maximum velocity of 41.37\u00a0\u00b1\u00a02.193\u00a0pmol\/min\/mg protein. In the present study, for the first time, the expression and functional characteristics of donkey PepT2 were evaluated, the results of which provide new insights and a better understanding of its crucial role in oligopeptide transport in donkeys.<\/jats:p>","DOI":"10.1111\/jpn.13615","type":"journal-article","created":{"date-parts":[[2021,7,27]],"date-time":"2021-07-27T12:46:05Z","timestamp":1627389965000},"page":"1165-1172","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Cloning, tissue distribution and functional characterization of the donkey (<i>Equus asinus<\/i>) oligopeptide transporter 2"],"prefix":"10.1111","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8459-1324","authenticated-orcid":false,"given":"Miaomiao","family":"Zhou","sequence":"first","affiliation":[{"name":"Liaocheng Research Institute of Donkey High\u2010Efficiency Breeding and Ecological Feeding College of Agriculture Liaocheng University  Liaocheng China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guiqin","family":"Liu","sequence":"additional","affiliation":[{"name":"Liaocheng Research Institute of Donkey High\u2010Efficiency Breeding and Ecological Feeding College of Agriculture Liaocheng University  Liaocheng China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenqiang","family":"Liu","sequence":"additional","affiliation":[{"name":"Liaocheng Research Institute of Donkey High\u2010Efficiency Breeding and Ecological Feeding College of Agriculture Liaocheng University  Liaocheng China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingxia","family":"Zhu","sequence":"additional","affiliation":[{"name":"Liaocheng Research Institute of Donkey High\u2010Efficiency Breeding and Ecological Feeding College of Agriculture Liaocheng University  Liaocheng China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changfa","family":"Wang","sequence":"additional","affiliation":[{"name":"Liaocheng Research Institute of Donkey High\u2010Efficiency Breeding and Ecological Feeding College of Agriculture Liaocheng University  Liaocheng China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,7,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.102.038315"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2017.12.021"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.1.284"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1211\/jpp.60.5.0002"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2990253"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.6b00462"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/132.1.38"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2017.02.005"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00054.2005"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1113\/EP085029"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu11102372"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-017-2234-z"},{"issue":"10","key":"e_1_2_8_14_1","doi-asserted-by":"crossref","first-page":"e02611","DOI":"10.1016\/j.heliyon.2019.e02611","article-title":"High fat diet modulates the protein content of nutrient transporters in the small intestine of mice: possible involvement of PKA and PKC activity","volume":"5","author":"Hijo A. 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