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A total of 350 (Ross 308) broiler breeder hens of 54\u00a0weeks randomly assigned to seven treatments, included control basal diet (C) without added Zn, C+ 100, and 130\u00a0mg Zn per kg of diet from Large (L) (100\u20131000\u00a0nm) and Small (S) (&lt;100\u00a0nm) particle size ZnO (LZnO100 and 130; SZnO100 and 130), C and SZnO100 challenged with lipopolysaccharide (C+LPS and SZnO100+LPS). Each diet was fed to five replicates consisting of ten birds each. The middle part of the duodenum, jejunum and ileum was used for morphological assessments. To assess the gene expression of ZO\u20101, OC and TNF\u2010\u03b1 in the jejunum samples were excised. Results showed that the supplementing 130\u00a0ppm SZnO increased VH:CD in the duodenum (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). VW in the duodenum and all the evaluated morphometric indices in jejunum and ileum were not affected by the dietary treatment (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a00.05). ZO\u20101\u00a0mRNA abundance in C+LPS group compared to SZnO100+LPS group was significantly decreased and increased by LPS and SZnO100 respectively. The SZnO\u2010100 increased OC gene expression in compare to C+LPS group. The expression of TNF\u2010\u03b1 in C+LPS treatment was higher than other groups (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). The lowest and the highest litter moisture and foot\u2010pad dermatitis (FPD) were observed in LZnO\u2010130 and C treatments respectively (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). Improving the physical properties of ZnO affect on VH:CD. Broiler breeder diet with ZnO enhance ZO\u20101, OC and mitigate TNF\u2010\u03b1 gene expression in jejunum maintenance of gut health in broiler breeders.<\/jats:p>","DOI":"10.1111\/jpn.13638","type":"journal-article","created":{"date-parts":[[2021,9,13]],"date-time":"2021-09-13T03:29:50Z","timestamp":1631503790000},"page":"772-782","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Effects of zinc dosage and particle size on gut morphology, tight junctions and TNF\u2010\u03b1 expression in broiler breeder hens"],"prefix":"10.1111","volume":"106","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7382-0742","authenticated-orcid":false,"given":"Masoud","family":"Barzegar","sequence":"first","affiliation":[{"name":"Department of Animal Science University of Tehran  Karaj Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mojtaba","family":"Zaghari","sequence":"additional","affiliation":[{"name":"Department of Animal Science University of Tehran  Karaj Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahdi","family":"Zhandi","sequence":"additional","affiliation":[{"name":"Department of Animal Science University of Tehran  Karaj Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mostafa","family":"Sadeghi","sequence":"additional","affiliation":[{"name":"Department of Animal Science University of Tehran  Karaj Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,9,12]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2013\u201003054"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.1812"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.13\u2010238717"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.35.12.1707"},{"key":"e_1_2_11_6_1","volume-title":"Ross 308. 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