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Intestinal integrity regulating effects, for example gene expression, of some feed ingredients are important key factors for that balance. We hypothesized that OEO affects the expression of genes associated with pigs\u2019 intestinal integrity. In four trials, a total of 86 pigs have been used. From weaning, the \u2018treated\u2019 group (<jats:italic>n<\/jats:italic>\u00a0=\u00a042) was additionally fed an oregano flavour additive [1500\u00a0mg\/kg (7.5% pure OEO)] within the basal diet. The \u2018control\u2019 group (<jats:italic>n<\/jats:italic>\u00a0=\u00a044) was kept under identical environmental conditions, except the OEO. At age of 6\u00a0months, pigs were slaughtered with an average weight of 111.1\u00a0\u00b1\u00a010.9\u00a0kg. In addition to automatically generated \u2018Fat\u2010o\u2010Meter\u2019 (AutoFOM) data, carcass quality factors have been measured manually. Valuable cuts of meat, such as ham and belly, were significantly reduced in the OEO group. Effects of OEO on pigs' haematologic parameters were very limited. For transcriptome analysis, the most interesting microarray expression results have been listed in a table (topTable). Selected genes were technically validated by qPCR. As a result, few significant differences in animal development and meat quality have been found between the OEO treated and the control group. Depending on OEO supplementation, we found 93 differently regulated genes in the jejunal tissue (70 up, 23 down) and 60 in the ileal tissue (48 up, 12 down). Just three genes (<jats:italic>GRIN3B<\/jats:italic> [<jats:italic>glutamate ionotropic receptor NMDA type subunit 3B<\/jats:italic>], TJP1\/<jats:italic>ZO<\/jats:italic>\u2010<jats:italic>1<\/jats:italic> [<jats:italic>tight junction protein ZO<\/jats:italic>\u2010<jats:italic>1<\/jats:italic>] and one uncharacterized gene) were affected by OEO both in jejunum and ileum. qPCR validation revealed <jats:italic>AKT serine<\/jats:italic>\/<jats:italic>threonine kinase 3<\/jats:italic> (<jats:italic>AKT3<\/jats:italic>), <jats:italic>Interferon<\/jats:italic> (<jats:italic>IFN<\/jats:italic>) \u2010<jats:italic>\u03b5<\/jats:italic>, \u2010<jats:italic>\u03c9<\/jats:italic>, <jats:italic>tight junction protein<\/jats:italic> (<jats:italic>TJP1<\/jats:italic>)\/<jats:italic>ZO<\/jats:italic>\u2010<jats:italic>1<\/jats:italic> (<jats:italic>ZO<\/jats:italic>\u2010<jats:italic>1<\/jats:italic>) to be upregulated in the jejunum and <jats:italic>C<\/jats:italic>\u2010<jats:italic>C motif chemokine ligand 21<\/jats:italic> (<jats:italic>CCL21<\/jats:italic>) was upregulated in the ileum of pigs that were supplemented with OEO. OEO supplementation had limited effects on pigs\u2019 performance traits. However, we were able to demonstrate that OEO alters the expression of genes associated with adaptive immune response in pigs\u2019 small intestine. These findings help to explain OEOs\u2019 beneficial impact on pigs\u2019 intestinal integrity.<\/jats:p>","DOI":"10.1111\/jpn.13639","type":"journal-article","created":{"date-parts":[[2021,10,9]],"date-time":"2021-10-09T04:42:03Z","timestamp":1633754523000},"page":"1017-1035","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Oregano essential oil showed limited effects on pigs' carcass quality and haematology whereas a transcriptome analysis revealed significant modulations in the jejunum and the ileum"],"prefix":"10.1111","volume":"106","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7327-1288","authenticated-orcid":false,"given":"Haiko Hendrik","family":"Hofmann","sequence":"first","affiliation":[{"name":"Institute of Animal Science Animal Breeding and Husbandry\/Animal Genetics Group University of Bonn  Bonn 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