{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T11:35:14Z","timestamp":1778153714132,"version":"3.51.4"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T00:00:00Z","timestamp":1637712000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFE0112700"],"award-info":[{"award-number":["2018YFE0112700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31501985"],"award-info":[{"award-number":["31501985"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31902179"],"award-info":[{"award-number":["31902179"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Chitosan nanoparticles (CNP), widely applied as oral drug\/gene\/vaccine carrier, were found to have anti\u2010inflammatory properties. In this study, the effects of CNP on lipopolysaccharide (LPS)\u2010induced intestinal damage in weaned piglets and the related mechanisms were investigated. Twenty\u2010four weaned piglets (Duroc\u00a0\u00d7\u00a0Landrace\u00a0\u00d7\u00a0Yorkshire, 21\u00a0\u00b1\u00a02\u00a0day of age, initial mass: 8.58\u00a0\u00b1\u00a00.59\u00a0kg) were randomly assigned into four groups: control, LPS, CNP and CNP\u00a0+\u00a0LPS. The control and LPS groups were fed a corn\u2013soybean meal\u2010based control diet, whereas the CNP and CNP\u00a0+\u00a0LPS groups were fed a control diet supplemented with 400\u00a0mg\/kg CNP. After 28\u00a0days of feeding, piglets in LPS and CNP\u00a0+\u00a0LPS groups were injected with LPS (100\u00a0\u03bcg\/kg); meanwhile, the piglets in control and CNP groups were injected with sterile saline. After 4\u00a0h from the LPS challenge, pigs were sacrificed to collect the intestinal samples for analysis. The results showed that CNP could attenuate the intestinal damages and inflammatory response stimulated by LPS treatment. LPS induced dramatically higher levels of CD177<jats:sup>+<\/jats:sup> neutrophils invasion in jejunum mucosa (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.01), which accompanied by increased secretion of marks of inflammation (<jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.01) compared with the control, whereas CNP administration obviously inhibited LPS\u2010induced CD177<jats:sup>+<\/jats:sup> neutrophils invasion (<jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.01) and secretion of marks of inflammation, such as interleukin\u20108 (<jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.05), intercellular adhesion molecule\u20101 (<jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.05) secretion in jejunum mucosa compared with LPS group. Moreover, CNP was shown to inhibit I\u03baB\u2010\u03b1 degradation in cytoplasm, which resulted in reduced nuclear translocation of NF\u2010\u03baB p65 in LPS\u2010challenged piglets. These findings suggest that CNP attenuates intestinal damage and inflammatory responses in LPS\u2010challenged weaned piglets by impairing the NF\u2010\u03baB signalling pathway.<\/jats:p>","DOI":"10.1111\/jpn.13664","type":"journal-article","created":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T08:56:44Z","timestamp":1637917004000},"page":"173-181","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Chitosan nanoparticles attenuate intestinal damage and inflammatory responses in LPS\u2010challenged weaned piglets via prevention of I\u03baB degradation"],"prefix":"10.1111","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7375-7969","authenticated-orcid":false,"given":"Yinglei","family":"Xu","sequence":"first","affiliation":[{"name":"Key Laboratory of Applied Technology on Green\u2010Eco\u2010Healthy Animal Husbandry of Zhejiang Province College of Animal Science and Technology \u2022 College of Veterinary Medicine Zhejiang A&amp;F University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8199-2694","authenticated-orcid":false,"given":"Qing","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Applied Technology on Green\u2010Eco\u2010Healthy Animal Husbandry of Zhejiang Province College of Animal Science and Technology \u2022 College of Veterinary Medicine Zhejiang A&amp;F University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pu","family":"Ge","sequence":"additional","affiliation":[{"name":"Pathophysiology Department of ChongQing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9399-9287","authenticated-orcid":false,"given":"Huiling","family":"Mao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Applied Technology on Green\u2010Eco\u2010Healthy Animal Husbandry of Zhejiang Province College of Animal Science and Technology \u2022 College of Veterinary Medicine Zhejiang A&amp;F University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caimei","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Applied Technology on Green\u2010Eco\u2010Healthy Animal Husbandry of Zhejiang Province College of Animal Science and Technology \u2022 College of Veterinary Medicine Zhejiang A&amp;F University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,11,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12446"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.06.027"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M701846200"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201745517"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2012.06.015"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfda.2014.10.008"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.shk.0000129759.58490.d6"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01997504"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/193229681300700228"},{"issue":"10","key":"e_1_2_8_11_1","first-page":"1447","article-title":"Interleukin\u20108 and neutrophil markers in colonic mucosa from patients with ulcerative colitis","volume":"87","author":"Izzo R. S.","year":"1992","journal-title":"America Journal of Gastroenterology"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2009.04.020"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.powtec.2017.02.021"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1051\/animres:2004018"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI124884"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a001651"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727\u2010214\u201044099"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pathol.4.110807.092135"},{"key":"e_1_2_8_19_1","first-page":"997","article-title":"Expression of interleukin\u20108 gene in inflammatory boweldisease is related to the histological grade of active inflammation","volume":"144","author":"Mazzucchelli L.","year":"1994","journal-title":"America Journal of Pathology"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/41131"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aninu.2017.06.003"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2017.07.087"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jddst.2018.10.022"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.03.001"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.1997.v113.pm9352851"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a000034"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/134.3.641"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aninu.2017.12.004"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.13118"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1001\/archpsyc.58.5.445"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209934"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41385\u2010018\u20100069\u20105"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00400.2008"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2013.106"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1567\u20105769(01)00010\u20108"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2016.02.015"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2004.04.056"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010biochem\u2010060713\u2010035600"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2015.06.001"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpn.13283"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2016-313535"}],"container-title":["Journal of Animal Physiology and Animal Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpn.13664","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jpn.13664","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpn.13664","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T20:16:13Z","timestamp":1692562573000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpn.13664"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,24]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1111\/jpn.13664"],"URL":"https:\/\/doi.org\/10.1111\/jpn.13664","archive":["Portico"],"relation":{},"ISSN":["0931-2439","1439-0396"],"issn-type":[{"value":"0931-2439","type":"print"},{"value":"1439-0396","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,24]]},"assertion":[{"value":"2021-02-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}