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At 17.5\u00a0days of incubation, fertile eggs were supplemented in the amniotic fluid with sterile saline or 3.5% threonine. Hatchlings were individually weighed, and <jats:italic>Salmonella<\/jats:italic> Enteritidis negative status was confirmed. At 2\u00a0days of age, half of the birds of each group were inoculated with sterile nutrient broth or <jats:italic>Salmonella<\/jats:italic> Enteritidis inoculum. Relative expression of sodium\u2010dependent glucose transporter 1 (<jats:italic>SGLT1<\/jats:italic>), glucose transporter 2 (<jats:italic>GLUT2<\/jats:italic>), di\u2010 and tri\u2010peptide transporter 1 (<jats:italic>PepT1<\/jats:italic>) and alanine, serine, cysteine, threonine transporter (<jats:italic>ASCT1<\/jats:italic>) was assessed at hatch, 2 and 9\u00a0days of age, i.e., before inoculation and 7\u00a0days post\u2010inoculation (dpi). At 9\u00a0days of age (7dpi), threonine increased <jats:italic>SGLT1<\/jats:italic> and <jats:italic>GLUT2<\/jats:italic> expression, whereas <jats:italic>GLUT2<\/jats:italic> expression decreased in <jats:italic>Salmonella<\/jats:italic>\u2010challenged birds. There was a significant interaction between threonine and <jats:italic>Salmonella<\/jats:italic> for <jats:italic>PepT1<\/jats:italic> and <jats:italic>ASCT1<\/jats:italic>. Threonine increased <jats:italic>PepT1<\/jats:italic> expression only in non\u2010challenged birds. In addition, <jats:italic>in ovo<\/jats:italic> supplementation increased expression of <jats:italic>ASCT1<\/jats:italic> regardless of post\u2010hatch inoculation; <jats:italic>Salmonella<\/jats:italic> inoculation resulted in decreased expression of <jats:italic>ASCT1<\/jats:italic> only in supplemented birds. The results suggest that while intra\u2010amniotic threonine administration in broiler embryos increases the expression of genes related to the absorption of monosaccharides and amino acids, <jats:italic>Salmonella<\/jats:italic> challenge may negatively affect the expression of protein related transporters in the ileum of broilers.<\/jats:p>","DOI":"10.1111\/jpn.13672","type":"journal-article","created":{"date-parts":[[2021,12,27]],"date-time":"2021-12-27T17:06:02Z","timestamp":1640624762000},"page":"395-402","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<i>In ovo<\/i> threonine supplementation affects ileal gene expression of nutrient transporters in broilers inoculated post\u2010hatch with <i>Salmonella<\/i> Enteritidis"],"prefix":"10.1111","volume":"106","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0067-813X","authenticated-orcid":false,"given":"Maria de F\u00e1tima de 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