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Previous studies summarized the literature on microbial AA patterns, most recently in 2017 (Sok et al.\n                    <jats:italic>Journal of Dairy Science<\/jats:italic>\n                    , 100, 5241\u20135249). The present study intended to identify the microbial AA pattern typical when feeding Central European diets and a maximum proportion of concentrate (PCO; dry matter (DM) basis) of 0.60. Data sets were created from the literature for liquid (LAB)\u2010 and particle (PAB)\u2010associated bacteria, total bacteria and protozoa, including 16, 9, 27 and 8\u00a0studies and 36, 21, 60 and 18 diets respectively. Because the only differences detected between LAB and PAB were slightly higher Phe and lower Thr percentages in PAB (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05), results for bacteria were pooled. A further data set evaluated AA\u2010N (AAN) as a proportion of total N in microbial fractions and a final data set estimated protozoal contributions to total microbial N (TMN) flow to the duodenum, which were used to calculate weighted TMN AA patterns. Protozoa showed higher Lys, Asp, Glu, Ile and Phe and lower Ala, Arg, Gly, Met, Ser, Thr and Val proportions than bacteria (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05). The AAN percentage of total N in bacteria and protozoa showed large, unexplained variations, averaging 79.0% and 70.6% (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&gt;\u00a00.05) respectively. Estimation of protozoal contribution to TMN resulted in a cattle\u2010specific mixed model including PCO and DM intake (DMI) per unit of metabolic body size (kg\n                    <jats:sup>0.75<\/jats:sup>\n                    ) as fixed effects (\n                    <jats:italic>RMSE<\/jats:italic>\n                    \u00a0=\u00a03.77). With moderate PCO and DMI between 80 and 180\u00a0g\/kg\n                    <jats:sup>0.75<\/jats:sup>\n                    , which corresponds to a DMI of approximately 10 to 25\u00a0kg in a cow with 650\u00a0kg body weight, protozoal contribution ranged between 9% and 26% of TMN. Within this range, the estimated protozoal contribution to TMN resulted in minor effects on the total microbial AA pattern.\n                  <\/jats:p>","DOI":"10.1111\/jpn.13676","type":"journal-article","created":{"date-parts":[[2021,12,29]],"date-time":"2021-12-29T02:49:25Z","timestamp":1640746165000},"page":"752-771","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Amino acid pattern of rumen microorganisms in cattle fed mixed diets\u2014An update"],"prefix":"10.1111","volume":"106","author":[{"given":"Nina","family":"Gresner","sequence":"first","affiliation":[{"name":"Institute of Animal Science University of Bonn  Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3156-7889","authenticated-orcid":false,"given":"Markus","family":"Rodehutscord","sequence":"additional","affiliation":[{"name":"Institute of Animal Science University of Hohenheim  Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8147-1060","authenticated-orcid":false,"given":"Karl\u2010Heinz","family":"S\u00fcdekum","sequence":"additional","affiliation":[{"name":"Institute of Animal Science University of Bonn  Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,12,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.69.255"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.2527\/2002.8082176x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022\u20100302(82)82467\u20103"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19740061"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2010\u20103703"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100396.2008.00901.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1071\/bi9731429"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/b205802m"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022\u20100302(92)78024\u20102"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas1974.381200x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2012\u20105323"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2013\u20106865"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas1988.664961x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.2527\/1993.7161596x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.4141\/A97\u2010043"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022\u20100302(92)77992\u20102"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19840014"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022\u20100302(82)82367\u20109"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022\u20100302(95)76626\u20107"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022\u20100302(93)77691\u20102"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022\u20100302(94)77311\u20102"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.2740270707"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022\u20105193(87)80240\u20100"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574\u20106941.2006.00190.x"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.nutr.23.011702.073318"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas1982.544837x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022\u20100302(82)82412\u20100"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022\u20100302(94)77310\u20100"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0377\u20108401(96)01059\u20100"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2018\u201015448"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2016\u201012531"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2013\u20107880"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1079\/NRR19980009"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731119000752"},{"key":"e_1_2_7_36_1","volume-title":"Anaerobic fungi \u2013 Growth and characterisation","author":"Gulati S. 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