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Eleutheroside E (EE) is a key part of <jats:italic>Acanthopanax senticosus<\/jats:italic> to exert pharmacological effects. This study aims to investigate the effects of EE on the barrier function in IPEC\u2010J2 cells and to determine the ability of EE to enhance the protective effect of barrier function against SBA exposure. The IPEC\u2010J2 cells were cultured in mediums with concentration of 0.1\u00a0mg\/ml EE, 0.5\u00a0ml\/ml SBA and 0.1\u00a0mg\/ml EE pre\u2010treated then treated with 0.5\u00a0mg\/ml SBA. Then, the transepithelial electric resistance (TEER) value, inflammatory cytokines mRNA expression, tight junction mRNA and protein expression were tested by epithelial Voltohm meter, q\u2010PCR and Western blot method respectively. The results showed that cells treated with 0.1\u00a0mg\/ml EE had lower permeability (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) while 0.5\u00a0mg\/ml SBA treatment had higher permeability through tested TEER, and higher tight junction proteins (Claudin\u20103 and ZO\u20101) expressions and genes (Claudin\u20103, Occludin and ZO\u20101) expressions (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) in 0.1\u00a0mg\/ml EE group. IPEC\u2010J2 cells pre\u2010treated with 0.1\u00a0mg\/ml EE could significantly improve the inflammatory response caused by 0.5\u00a0mg\/ml SBA by up\u2010regulation for IL\u201010, TGF\u2010\u03b2, and down\u2010regulation gene expression of IL\u20106, TNF\u2010\u03b1 and IFN\u2010\u03b3 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). In conclusion, 0.1\u00a0mg\/ml EE can improve the mechanical barrier function and could protect the effects while 0.5\u00a0mg\/ml of SBA\u2010induced barrier dysfunction in IPEC\u2010J2.<\/jats:p>","DOI":"10.1111\/jpn.13677","type":"journal-article","created":{"date-parts":[[2022,1,11]],"date-time":"2022-01-11T05:27:16Z","timestamp":1641878836000},"page":"664-670","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Mitigative effects of Eleutheroside E against the mechanical barrier dysfunction induced by soybean agglutinin in IPEC\u2010J2 cell line"],"prefix":"10.1111","volume":"106","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2292-8603","authenticated-orcid":false,"given":"Bao","family":"Zhao","sequence":"first","affiliation":[{"name":"Jilin Provincial Key Lab of Animal Nutrition and Feed Science Key Lab of Animal Production &amp; Product Quality, and Security, Ministry of Education Jilin Provincial Swine Industry Technical Innovation Center College of Animal Science and Technology Jilin Agricultural University  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yueli","family":"Fan","sequence":"additional","affiliation":[{"name":"Jilin Provincial Key Lab of Animal Nutrition and Feed Science Key Lab of Animal Production &amp; Product Quality, and Security, Ministry of Education Jilin Provincial Swine Industry Technical Innovation Center College of Animal Science and Technology Jilin Agricultural University  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huijuan","family":"Li","sequence":"additional","affiliation":[{"name":"Jilin Provincial Key Lab of Animal Nutrition and Feed Science Key Lab of Animal Production &amp; Product Quality, and Security, Ministry of Education Jilin Provincial Swine Industry Technical Innovation Center College of Animal Science and Technology Jilin Agricultural University  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Changchun University of Science and Technology  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Han","sequence":"additional","affiliation":[{"name":"Jilin Provincial Key Lab of Animal Nutrition and Feed Science Key Lab of Animal Production &amp; Product Quality, and Security, Ministry of Education Jilin Provincial Swine Industry Technical Innovation Center College of Animal Science and Technology Jilin Agricultural University  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7604-1894","authenticated-orcid":false,"given":"Dongsheng","family":"Che","sequence":"additional","affiliation":[{"name":"Jilin Provincial Key Lab of Animal Nutrition and Feed Science Key Lab of Animal Production &amp; Product Quality, and Security, Ministry of Education Jilin Provincial Swine Industry Technical Innovation Center College of Animal Science and Technology Jilin Agricultural University  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,1,10]]},"reference":[{"issue":"6","key":"e_1_2_9_2_1","first-page":"934183","article-title":"Eleutheroside E, an active component of eleutherococcus senticosus, ameliorates insulin resistance in type 2 diabetic db\/db mice","volume":"2013","author":"Ahn J.","year":"2013","journal-title":"Evidence\u2010based Complementary and Alternative Medicine"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.2741\/3413"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01288.2004"},{"key":"e_1_2_9_5_1","first-page":"242","volume-title":"Inflammatory bowel disease and the apical junctional complex","author":"Bruewer M.","year":"2010"},{"issue":"1","key":"e_1_2_9_6_1","doi-asserted-by":"crossref","first-page":"a029314","DOI":"10.1101\/cshperspect.a029314","article-title":"Cell biology of tight junction barrier regulation and mucosal disease","volume":"10","author":"Buckley A. 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