{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T16:53:24Z","timestamp":1776876804874,"version":"3.51.2"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007831","name":"University of Tabriz","doi-asserted-by":"publisher","award":["ANIM 834"],"award-info":[{"award-number":["ANIM 834"]}],"id":[{"id":"10.13039\/501100007831","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to investigate the influence of dietary supplementation of bovine lactoferrin (bLF) on growth performance, carcass traits, histomorphology of jejunum, immune function and hepatic and splenic gene expression of interferon\u2010gamma (IFN\u2010\u03b3) and interleukine\u20102 (IL\u20102) in broiler chickens. A total of 240 one\u2010day\u2010old Ross 308\u00a0male broiler chickens were randomly allotted into six dietary treatments with four replicate pens (10 chicks per pen) and fed experimental diet in 3 feeding phases (starter: d 0\u201310, grower: d 11\u201324 and finisher: d 25\u201342). The experimental treatments were (1) corn\u2013soya bean meal\u2010based basal diet (control), (2\u20135) basal diet supplemented with 200, 400, 600, 800\u00a0mg\/kg bLF, respectively, and (6) basal diet supplemented with 200\u00a0mg\/kg oxytetracycline (OTC). The average body weight gain (ABWG) of broilers fed 800\u00a0mg\/kg bLF was 8.48% higher than those fed a corn\u2013soybean meal\u2010based diet during the starter period (d 0\u201310) (linear effect, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.002; quadratic effect, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.24). Average daily feed intake (ADFI) and the feed conversion ratio (FCR) were not affected (<jats:italic>p<\/jats:italic>&gt;0.05) by bLF supplementation. At 42\u00a0days of age, the breast meat percentage and carcass yield of broilers fed 800\u00a0mg\/kg bLF compared with the control group significantly increased by 9.51% and 6.03% respectively (<jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.05). Compared with the chicks fed the control diet, the chicks fed diets supplemented with bLF had higher villus height, muscle thickness and villus surface area (<jats:italic>p\u00a0<\/jats:italic>&gt;\u00a00.05). Dietary bLF inclusion increased the total immunoglobulin (IgT) titre against sheep red blood cells (SRBCs) antigen (linear effect, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.031; quadratic effect, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.035) and improved the phytohaemagglutinin\u2010P (PHA\u2010P)\u2010skin test of broilers. Compared with the control, bLF enhanced the gene expression of IFN\u2010\u03b3 in spleen (<jats:italic>p\u00a0<\/jats:italic>=\u00a00.048, linear effect, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.009; quadratic effect, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.093) and liver (<jats:italic>p\u00a0<\/jats:italic>=\u00a00.012, linear effect, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.008; quadratic effect, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.01) and IL\u20102 expression in spleen (<jats:italic>p\u00a0<\/jats:italic>=\u00a00.021, linear effect, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.026; quadratic effect, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.103). The bLF supplementation had no effect on IL\u20102\u00a0gene expression in liver (<jats:italic>p\u00a0<\/jats:italic>&gt;\u00a00.05, linear effect, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.213; quadratic effect, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.159). In conclusion, we found that supplementation of broiler diets with 800\u00a0mg\/kg bLF can improve the growth performance, carcass yield, cell\u2010mediated and antibody\u2010mediated immune responses and enhance the IL\u20102 and IFN\u2010\u03b3 gene expression of broilers.<\/jats:p>","DOI":"10.1111\/jpn.13683","type":"journal-article","created":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T09:35:44Z","timestamp":1643708144000},"page":"200-213","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Supplementation of broiler chicken diets with bovine lactoferrin improves growth performance, histological parameters of jejunum and immune\u2010related gene expression"],"prefix":"10.1111","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8764-2895","authenticated-orcid":false,"given":"Majid","family":"Olyayee","sequence":"first","affiliation":[{"name":"Department of Animal Science Faculty of Agriculture University of Tabriz  Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arash","family":"Javanmard","sequence":"additional","affiliation":[{"name":"Department of Animal Science Faculty of Agriculture University of Tabriz  Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hossein","family":"Janmohammadi","sequence":"additional","affiliation":[{"name":"Department of Animal Science Faculty of Agriculture University of Tabriz  Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruhollah","family":"Kianfar","sequence":"additional","affiliation":[{"name":"Department of Animal Science Faculty of Agriculture University of Tabriz  Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sadegh","family":"Alijani","sequence":"additional","affiliation":[{"name":"Department of Animal Science Faculty of Agriculture University of Tabriz  Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyed Ali","family":"Mir Ghelenj","sequence":"additional","affiliation":[{"name":"Department of Animal Science Faculty of Agriculture Urmia University  Urmia Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.21608\/zvjz.2016.7881"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/138161209788453202"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1567\u20105769(01)00189\u20108"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.17221\/1978\u2010VETMED"},{"issue":"1","key":"e_1_2_10_6_1","article-title":"Effect of bovine lactoferrin on growth performance and intestinal histologic features of broiler","volume":"41","author":"Aguirre A. 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