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In this study, we established an in vitro high glucose model of intramuscular adipocytes of sheep to investigate the expression of cannabinoid receptor 1 (CB1) gene, fatty acid\u2010binding protein 4 (FABP4) gene, lipid metabolism\u2010associated genes (acetyl\u2010CoA carboxylase [ACC], fatty acid synthase [FAS], and stearoyl\u2010CoA desaturase 1 [SCD1]), and transcription factors (liver X receptor [LXR\u03b1]), sterol regulatory element\u2010binding transcription factor 1 [SREBF\u20101], and carbohydrate\u2010responsive element\u2010binding protein [ChREBP]) as well as the changes in the lipid and triglyceride (TG) levels in intramuscular adipocytes. The results showed that the differentiated mature adipocytes had a spherical shape, and the number and volume of the lipid droplets gradually increased over time under high glucose conditions. The lipid and TG levels in intramuscular adipocytes of sheep continuously increased under high glucose conditions. Furthermore, CB1, FABP4, ACC, FAS, SCD1, LXR\u03b1, SREBF\u20101, and ChREBP were highly expressed under high glucose conditions, suggesting that the energetic nutrients also affect the expression of the CB1 gene, which works\u00a0in coordination with lipid metabolism\u2010associated genes and are\u00a0beneficial for lipid deposition in the intramuscular adipocytes of sheep.<\/jats:p>","DOI":"10.1111\/jpn.13750","type":"journal-article","created":{"date-parts":[[2022,6,27]],"date-time":"2022-06-27T05:09:52Z","timestamp":1656306592000},"page":"444-452","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Expression of key genes involved in lipid deposition in intramuscular adipocytes of sheep under high glucose 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