{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T23:06:41Z","timestamp":1784934401474,"version":"3.55.0"},"reference-count":50,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,8,23]],"date-time":"2022-08-23T00:00:00Z","timestamp":1661212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mastitis is one of the most common diseases of dairy cattle and can be caused by physical stress, chemicals and microbial infection. <jats:italic>Staphylococcus aureus<\/jats:italic> is the most common pathogens that induce mastitis in dairy cattle. In this study, bovine mammary epithelial cells (BMECs) were treated either with lipoteichoic acid (LTA, 30\u2009\u00b5g\/ml) or 1\u2009\u00d7\u2009phosphate\u2010buffer saline (PBS, control) and RNA\u2010Seq was applied to explore the effect of LTA on the expression microRNAs (miRNAs) in BMECs. Compared to the control group, 43 miRNAs were significantly up\u2010regulated and eight miRNAs were significantly down\u2010regulated. Additionally, 724 genes were significantly up\u2010regulated and 13 genes were significantly down\u2010regulated in LTA group relative to the control group. Bta\u2010miR\u2010196a, bta\u2010miR\u20102285aj\u20105p, bta\u2010miR\u2010143, bta\u2010miR\u20102433, bta\u2010miR\u20102284f\u00a0and bta\u2010miR\u20102368\u20103p were selected from 51 differentially expressed miRNAs and are discussed in this manuscript. Target gene prediction revealed that the target genes of these six miRNAs were all differentially expressed, including MT1E, SPDYA, FGL1, TLR2, PAPOLG, ZDHHC17\u00a0and SMC4. Subsequently, the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the target genes with differentially expressed miRNAs were enriched in mitogen\u2010activated protein kinase (MAPK) signalling pathway, rheumatoid arthritis and cancer. Therefore, the results of this study provided new evidences for the molecular mechanism of LTA\u2010induced mastitis, which may provide new targets for the diagnosis and treatment of mastitis in dairy cattle.<\/jats:p>","DOI":"10.1111\/jpn.13760","type":"journal-article","created":{"date-parts":[[2022,8,23]],"date-time":"2022-08-23T12:34:56Z","timestamp":1661258096000},"page":"463-474","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Analysis of differentially expressed microRNAs in bovine mammary epithelial cells treated with lipoteichoic acid"],"prefix":"10.1111","volume":"107","author":[{"given":"Puxiu","family":"Shen","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Henan Agricultural University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingcheng","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Henan 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