{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T04:52:34Z","timestamp":1792212754792,"version":"4.4.0"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T00:00:00Z","timestamp":1662940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study was conducted to investigate the effect of in ovo administration of a mixture of <jats:italic>Astragalus<\/jats:italic> polysaccharide (APS) and Newcastle disease vaccine (NDV) on growth performance, intestinal development, and mucosal immunity in newly hatched chicks. Six hundred specific\u2010pathogen\u2010free (SPF) Leghorn fertilised eggs were incubated in a commercial hatchery and divided into four groups: (a) control group injected with 1\u2009ml of 0.9% physiological saline, (b) APS group injected with 1\u2009ml of 1\u2009mg\/ml APS solution, and (c) NDV group injected with 1\u2009ml of 10<jats:sup>4.0<\/jats:sup> EID<jats:sub>50<\/jats:sub>\/dose of NDV solution, and (d) APS\u2009+\u2009NDV group injected with a mixture of 0.5\u2009ml of 2\u2009mg\/ml APS plus 0.5\u2009ml 10<jats:sup>4.0<\/jats:sup> EID<jats:sub>50<\/jats:sub>\/dose ND vaccine (NDV) on Day 18.5 of incubation. The results showed that in ovo injection of APS or the mixture of APS and NDV increased the body weight at 1 day (IW) and final weight (FW) at 28 days and increased the feed conversion ratio (FCR) at 1\u20137, 8\u201314, 15\u201321, and 1\u201328 days of age. The villus height (VH) was increased (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05), and the crypt depth (CD) was decreased (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) in the duodenum compared with the control group. The VH\/CD ratios were increased (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) in the APS\u2009+\u2009NDV group compared with controls, NDV group, and APS group on d3. The levels of slgA in washings were increased (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) on Days 3, 7, 14, 21, and 28, and the number of IgA<jats:sup>+<\/jats:sup> cells in the duodenum was increased on Days 7, 14, 21, and 28. In addition, the IgA<jats:sup>+<\/jats:sup> cells were promoted from the villus root to the apex in the APS\u2009+\u2009NDV group. It can be concluded that in ovo administration of NDV conjugated with APS compared with NDV alone may be more effective in promoting growth performance and intestinal mucosal immunity.<\/jats:p>","DOI":"10.1111\/jpn.13771","type":"journal-article","created":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T15:24:46Z","timestamp":1662996286000},"page":"897-906","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Effect of in ovo administration of Newcastle disease vaccine conjugated with <i>Astragalus<\/i> polysaccharide on growth performance, intestinal development, and mucosal immunity in broiler chickens"],"prefix":"10.1111","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8137-7114","authenticated-orcid":false,"given":"Ligang","family":"Xue","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Jilin Agricultural Science and Technology University Jilin China"},{"name":"College of Animal Science and Technology Jilin Agricultural University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingnan","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Public Health Jilin Medical University Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Jilin Agricultural University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weimin","family":"Luan","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Jilin Agricultural University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8227-5934","authenticated-orcid":false,"given":"Shubao","family":"Yang","sequence":"additional","affiliation":[{"name":"Basic Medical College Jilin Medical University Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1053\/rvsc.2000.0394"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1590\/1806-9061-2015-0198"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2005.04.037"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pev182"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2011-01488"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2010.02.004"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1637\/10540-032613-Reg.1"},{"issue":"3","key":"e_1_2_10_9_1","first-page":"382","article-title":"Astragalus polysaccharide perseveres Cytomembrane capacity against Newcastle disease virus infection","volume":"35","author":"Ge M.","year":"2015","journal-title":"Pakistan Veterinary Journal"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.2307\/1593021"},{"key":"e_1_2_10_11_1","first-page":"175","article-title":"Enteric diseases of poultry with special attention to Clostridium perfringens","volume":"31","author":"Hafez H. 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