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One hundred and eighty fertile eggs were divided as follows: the control group, 3\u2009mg\/egg ISF (low dose)\u00a0and 6\u2009mg\/egg ISF (high dose) on the 18th\u00a0day of incubation. The results demonstrated that in ovo inclusion of 6\u2009mg of ISF significantly increased hatchability and hatch weight. Both doses of ISF inclusion elevated the serum glutathione peroxidase and slightly decreased malondialdehyde compared to the control group. The high dose of ISF brings higher villus height and a higher villus\/crypt ratio in chicks. Moreover, the mRNA levels of tumour necrosis factor\u2010 \u03b1\u00a0and interferon\u2010gamma\u00a0in the spleen were significantly decreased. The ISF treatments showed an improvement in intestinal enzyme expression levels of sucrose isomaltase\u00a0and mucin 2 \u00a0as well as tight junction protein (TJ) mRNA expression of claudin\u20101 at high doses of ISF (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) when compared with the other groups. Furthermore, the mRNA level of IGF\u20101 was increased in the high doses of ISF compared to the control. Overall, these findings indicate that in ovo administration of ISF on the 18th\u00a0day of incubation enhances hatchability, antioxidant status\u00a0and intestinal morphometrics in hatched chicks and modulates the expression of proinflammatory cytokines, TJs\u00a0and insulin\u2010like growth factor. In addition, the sustainability of antioxidants and other positive effects of ISF may increase chick viability and growth performance.<\/jats:p>","DOI":"10.1111\/jpn.13850","type":"journal-article","created":{"date-parts":[[2023,7,1]],"date-time":"2023-07-01T04:53:37Z","timestamp":1688187217000},"page":"1381-1391","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["In ovo injection of soy isoflavones on hatching performance and intestinal development of newly hatched chicks"],"prefix":"10.1111","volume":"107","author":[{"given":"Ezaldeen","family":"Abdelghani","sequence":"first","affiliation":[{"name":"Research Center for Livestock Environmental Control and Smart Production, College of Animal Science and Technology Nanjing Agricultural University Nanjing Jiangsu People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed A.","family":"Fathi","sequence":"additional","affiliation":[{"name":"Research Center for Livestock Environmental Control and Smart Production, College of Animal Science and Technology Nanjing Agricultural University Nanjing Jiangsu People's Republic of China"},{"name":"Poultry Breeding Department, Animal Production Research Institute Agricultural Research Centre  Dokki Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaojian","family":"Li","sequence":"additional","affiliation":[{"name":"Research Center for Livestock Environmental Control and Smart Production, College of Animal Science and Technology Nanjing Agricultural University Nanjing Jiangsu People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengyuan","family":"Dai","sequence":"additional","affiliation":[{"name":"Research Center for Livestock Environmental Control and Smart Production, College of Animal Science and Technology Nanjing Agricultural University Nanjing Jiangsu People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yansen","family":"Li","sequence":"additional","affiliation":[{"name":"Research Center for Livestock Environmental Control and Smart Production, College of Animal Science and Technology Nanjing Agricultural University Nanjing Jiangsu People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6158-1254","authenticated-orcid":false,"given":"Chunmei","family":"Li","sequence":"additional","affiliation":[{"name":"Research Center for Livestock Environmental Control and Smart Production, College of Animal Science and Technology Nanjing Agricultural University Nanjing Jiangsu People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,6,30]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/1806-9061-2018-0940"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0043933916000532"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1079\/095442299108728875"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/68.6.1364S"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.4161\/21688370.2014.982426"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsbl.2013.0757"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pey609"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani9010025"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani12162042"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2021.101103"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.7b06069"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpn.13181"},{"key":"e_1_2_9_14_1","doi-asserted-by":"crossref","unstructured":"Fathi M. 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