{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T21:45:15Z","timestamp":1782769515122,"version":"3.54.5"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T00:00:00Z","timestamp":1698796800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effects of in ovo lactoferrin (Lf) injection on some physiological parameters and immune response of posthatch chicks were investigated. Live embryonated Fayoumi chicken eggs (<jats:italic>n<\/jats:italic>\u2009=\u2009600) were randomly allocated into four groups. The first group as a control was noninjected eggs, the second\u00a0group was only injected with 0.1\u2009mL of NaCl 0.75% solution, and the third\u00a0and fourth\u00a0groups were injected with 50 and 100\u2009\u00b5L Lf dissolved in 0.1\u2009mL saline solution respectively. The eggs were injected on Day 15 of incubation in the amnion. The results illustrated that the hatchability of eggs in two Lf groups was significantly higher than in the control, NaCl groups. The residual yolk in chicks injected with Lf (100\u2009\u00b5L\/egg) was significantly lower than the control group (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). In ovo Lf injection improved lipid profile, liver function, antioxidant indices, blood haematology, serum immunoglobulins and jejunum histomorphometry compared to the control group (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). In ovo injection of Lf decreased significantly (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) of pathogenic bacteria in residual yolk such as<jats:italic>Salmonella<\/jats:italic>,\u00a0<jats:italic>Shigella<\/jats:italic>\u00a0and<jats:italic>Coliform<\/jats:italic>compared to the control group. In conclusion, in ovo Lf injection can improve the hatchability, lipid profile, immune response and antioxidant indices and decline pathogens in the residual yolk, thus boosting the health status of newly hatched Fayoumi chicks.<\/jats:p>","DOI":"10.1111\/jpn.13901","type":"journal-article","created":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T07:20:11Z","timestamp":1698823211000},"page":"395-402","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effect of in ovo lactoferrin injection in Fayoumi chicken eggs on immune response and some physiological parameters in posthatch chicks"],"prefix":"10.1111","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5075-7739","authenticated-orcid":false,"given":"Mohamed A.","family":"Fathi","sequence":"first","affiliation":[{"name":"Agricultural Research Centre Animal Production Research Institute Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adel M.","family":"Abdelsalam","sequence":"additional","affiliation":[{"name":"Agricultural Research Centre Animal Production Research Institute Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waleed","family":"Elnagar","sequence":"additional","affiliation":[{"name":"Agricultural Research Centre Animal Production Research Institute Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4557-9816","authenticated-orcid":false,"given":"Hamada","family":"Elwan","sequence":"additional","affiliation":[{"name":"Animal and Poultry Production Department, Faculty of Agriculture Minia University El\u2010Minya Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4430-1492","authenticated-orcid":false,"given":"Shaaban S.","family":"Elnesr","sequence":"additional","affiliation":[{"name":"Department of Poultry Production, Faculty of Agriculture Fayoum University Fayoum Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpn.13850"},{"key":"e_1_2_8_3_1","volume-title":"Melanomas: New insights for the healthcare professional: 2013 edition","author":"Acton Q. A.","year":"2013"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.17221\/1978-VETMED"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.5455\/jabet.2021.d123"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.5604\/17322693.1061635"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.21608\/aijpms.2021.78232.1076"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1139\/o11-071"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-7020-4226-3.00010-X"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10534-014-9779-7"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1139\/o01-212"},{"key":"e_1_2_8_12_1","first-page":"38","volume-title":"Diseases of swine","author":"Calhoun M. L.","year":"1975"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199401000-00025"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2011.11.002"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.21608\/scvmj.2017.62452"},{"issue":"87","key":"e_1_2_8_16_1","first-page":"201","article-title":"Bovine lactoferrin ameliorates antioxidant esterase activity and 8\u2010isoprostane levels in high\u2010cholesterol\u2010diet fed rats","volume":"2019","author":"Faridvand Y.","year":"2019","journal-title":"International Journal for Vitamin and Nutrition Research"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1095-6433(99)00122-1"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.idairyj.2018.09.004"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.01221"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27092941"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu10111628"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.2131\/jts.37.1"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/09168451.2015.1091713"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpn.13683"},{"issue":"4","key":"e_1_2_8_25_1","first-page":"229","article-title":"Effect of lactoferrin supplementation on blood profile, immunity and growth performance in newly born calves","volume":"22","author":"Omar N. A.","year":"2022","journal-title":"Journal of the Egyptian Veterinary Medical Association"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985820960127"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.15419\/bmrat.v9i2.727"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani11123491"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.09-0324"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2006.034876"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:BIOM.0000027697.83706.32"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.0690001"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2013-7505"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2009.01.003"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2005-544"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-005-5369-8"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0043933914000610"},{"issue":"3","key":"e_1_2_8_38_1","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1002\/hep.23476","article-title":"Lactoferrin protects against acetaminophen\u2010induced liver injury in mice","volume":"24","author":"Yin H.","year":"2010","journal-title":"Hepatology"}],"container-title":["Journal of Animal Physiology and Animal Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpn.13901","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T05:18:07Z","timestamp":1730438287000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpn.13901"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11]]},"references-count":37,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["10.1111\/jpn.13901"],"URL":"https:\/\/doi.org\/10.1111\/jpn.13901","archive":["Portico"],"relation":{},"ISSN":["0931-2439","1439-0396"],"issn-type":[{"value":"0931-2439","type":"print"},{"value":"1439-0396","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11]]},"assertion":[{"value":"2023-09-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}