{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T05:39:45Z","timestamp":1764049185672,"version":"3.45.0"},"reference-count":67,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T00:00:00Z","timestamp":1749600000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Analysing the development of a digestive system and enzyme activity is crucial for understanding the digestive physiology of larval fish and determining optimal feeding practices. The white\u2010barred goby (\n                    <jats:italic>Amblygobius phalaena<\/jats:italic>\n                    ), a popular marine ornamental fish, has established breeding and larval rearing techniques, but research on its digestive physiology remains limited, which is essential for enhancing commercial production efficiency. This study aimed to elucidate the histological structure of the digestive tract and changes in enzyme activity in white\u2010barred goby larvae to understand the progression of their digestive functions, providing a foundation for future weaning studies. Histological observations revealed that 6\u20138\u2009h before hatching, the digestive tract appeared as a simple straight tube, with the liver, pancreas and gallbladder already formed. Gastric glands developed by 35 days post\u2010hatch (dph), at which point the larvae reached a total length of 15.70\u201318.50\u2009mm. The stomach reached full development at 40\u2009dph (20.11\u201325.12\u2009mm). Analysis of digestive enzyme activity showed that the total activities of trypsin, amylase, lipase and pepsin in white\u2010barred goby larvae gradually increased with growth. Lipase and pepsin specific activities peaked at 40\u2009dph (19.58\u201325.13\u2009mm). Based on these results, we recommend to introduce artificial feed starting from 35\u2009dph (15.70\u201318.50\u2009mm). The findings of this study are significant for improving larviculture and establishing the weaning time of white\u2010barred goby larvae.\n                  <\/jats:p>","DOI":"10.1111\/jpn.14135","type":"journal-article","created":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T18:36:37Z","timestamp":1749666997000},"page":"1225-1237","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of the Digestive System and Digestive Enzymes in the Marine Ornamental Fish White\u2010Barred Goby\n                    <i>Amblygobius phalaena<\/i>"],"prefix":"10.1111","volume":"109","author":[{"given":"Pei\u2010Sheng","family":"Chiu","sequence":"first","affiliation":[{"name":"Mariculture Research Center Fisheries Research Institute, Ministry of Agriculture Tainan Taiwan"},{"name":"Department of Aquaculture National Pingtung University of Science and Technology Neipu Pingtung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Hsu","sequence":"additional","affiliation":[{"name":"Mariculture Research Center Fisheries Research Institute, Ministry of Agriculture Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shine\u2010Wei","family":"Ho","sequence":"additional","affiliation":[{"name":"Mariculture Research Center Fisheries Research Institute, Ministry of Agriculture Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng\u2010Hsuan","family":"Huang","sequence":"additional","affiliation":[{"name":"Mariculture Research Center Fisheries Research Institute, Ministry of Agriculture Tainan Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yen\u2010Chun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Aquaculture National Pingtung University of Science and Technology Neipu Pingtung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5111-4046","authenticated-orcid":false,"given":"Yu\u2010Hung","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Aquaculture National Pingtung University of Science and Technology Neipu Pingtung Taiwan"},{"name":"International Program in Ornamental Fish Technology and Aquatic Animal Heath National Pingtung University of Science and Technology Neipu Pingtung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,6,11]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"245","volume-title":"The Fishes of the Japanese Archipelago","author":"Akihito P.","year":"1984"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmars.2022.907568"},{"key":"e_1_2_8_4_1","unstructured":"Biota.2024. \u201cWhat We Do? 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