{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:05:06Z","timestamp":1788303906036,"version":"build-2803163510"},"reference-count":52,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,7,13]],"date-time":"2025-07-13T00:00:00Z","timestamp":1752364800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Raw meat\u2010based diets (RMBDs) for pets, which contain raw meat from livestock, aquatic species, or wild\u2010captive animals, are known to harbour feed\u2010borne pathogens and antimicrobial\u2010resistant (AMR) bacteria. This study investigated the contamination of extended\u2010spectrum \u03b2\u2010lactamase (ESBL)\u2010producing\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    ,\n                    <jats:italic>Salmonella<\/jats:italic>\n                    ,\n                    <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    , and\n                    <jats:italic>E. faecium<\/jats:italic>\n                    in Thai RMBD products, focusing on their AMR phenotypic and genotypic properties. A total of 50 RMBD samples and five fresh\u2010cooked diets (FCDs) were analysed for total aerobic colony counts (ACC) and the presence of third\u2010generation cephalosporins (3GC)\u2010resistant\n                    <jats:italic>E. coli<\/jats:italic>\n                    ,\n                    <jats:italic>Salmonella<\/jats:italic>\n                    ,\n                    <jats:italic>E. faecalis<\/jats:italic>\n                    , and\n                    <jats:italic>E. faecium<\/jats:italic>\n                    . The AMR phenotypes of isolated bacteria were determined using antimicrobial susceptibility testing and ESBL production, while\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX\u2010M<\/jats:sub>\n                    and\n                    <jats:italic>mcr<\/jats:italic>\n                    genes were detected by PCR assays. ACCs in RMBD samples were higher than those in FCD samples. The RMBD products tested positive for 3GC\u2010resistant\n                    <jats:italic>E. coli<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200940, 80.0%),\n                    <jats:italic>Salmonella<\/jats:italic>\n                    spp. (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200935, 70.0%),\n                    <jats:italic>E. faecalis<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200950, 100%), and\n                    <jats:italic>E. faecium<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200942, 84.0%). All 3GC\u2010resistant\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates were ESBL producers carrying\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX\u2010M<\/jats:sub>\n                    group 1 and group 9. Among the\n                    <jats:italic>Salmonella<\/jats:italic>\n                    isolates, five ESBL\u2010producing\n                    <jats:italic>S<\/jats:italic>\n                    . Infantis isolates carried\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX\u2010M<\/jats:sub>\n                    group 9. Two ESBL\u2010producing\n                    <jats:italic>E. coli<\/jats:italic>\n                    and seven\n                    <jats:italic>Salmonella<\/jats:italic>\n                    isolates exhibited colistin resistance. No vancomycin resistance was observed in the enterococcal isolates. These findings underscore the high prevalence of\n                    <jats:italic>Salmonella<\/jats:italic>\n                    and ESBL\u2010producing\n                    <jats:italic>E. coli<\/jats:italic>\n                    in RMBD products, emphasising the potential health risks associated with RMBD consumption for pets. This study highlights the need for awareness and preventive measures to mitigate AMR spread through pet foods.\n                  <\/jats:p>","DOI":"10.1111\/jpn.70004","type":"journal-article","created":{"date-parts":[[2025,7,13]],"date-time":"2025-07-13T19:42:56Z","timestamp":1752435776000},"page":"1284-1297","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Contamination With Antimicrobial\u2010Resistant\n                    <i>Escherichia coli<\/i>\n                    ,\n                    <i>Salmonella<\/i>\n                    , and\n                    <i>Enterococcus<\/i>\n                    in Raw Meat\u2010Based Diets for Pets"],"prefix":"10.1111","volume":"109","author":[{"given":"Rusmin","family":"Indra","sequence":"first","affiliation":[{"name":"Department of Veterinary Microbiology, Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1989-0379","authenticated-orcid":false,"given":"Parinya","family":"Sroithongkham","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology, Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7835-2191","authenticated-orcid":false,"given":"Chavin","family":"Leelapsawas","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology, Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jitrapa","family":"Yindee","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology, Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siraphatson","family":"Wetchasirigul","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health, Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rungtip","family":"Chuanchuen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health, Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand"},{"name":"Research Unit in Microbial Food Safety and Antimicrobial Resistance, Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8979-2440","authenticated-orcid":false,"given":"Pattrarat","family":"Chanchaithong","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology, Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand"},{"name":"Research Unit in Microbial Food Safety and Antimicrobial Resistance, Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/4674235"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jas\/sky235"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cofs.2024.101165"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12852"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12351"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2009-3273"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00080"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.104535"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01009-09"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2019.0011"},{"issue":"1","key":"e_1_2_9_12_1","first-page":"40","article-title":"Risk Factors of Enterococcal Bacteriuria in Cats: A Retrospective Study","volume":"64","author":"Clark H.","year":"2023","journal-title":"Canadian Veterinary Journal"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/1758-2229.12019"},{"key":"e_1_2_9_14_1","volume-title":"CLSI M100\u2010ED33:2023 Performance Standards for Antimicrobial Susceptibility Testing","author":"Clinical Laboratory Standards Institute (CLSI)","year":"2023"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp498"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/jsap.13000"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114511002765"},{"key":"e_1_2_9_18_1","unstructured":"EC 2073\/2005. 2005. 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