{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T14:49:13Z","timestamp":1788965353620,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Intensive broiler production may lead to perturbations of gut function and health. The maintenance of intestinal homeostasis in broilers through the use of dietary phytogenic components such as isoquinoline alkaloids (IQs) is an emerging topic of scientific investigations. In this respect, IQs effects on the underlying mechanisms involved in gut antioxidant capacity, barrier integrity and inflammatory response still remain unclear. This study aimed to investigate the effect of dietary administration of an IQs blend on the expression of genes relevant for gut antioxidant response, barrier function, and inflammatory status, along the intestine of 35\u2010days\u2010old broilers. One hundred eighty\u2010two one\u2010day\u2010old Ross 308 broilers were randomly distributed into 2 experimental treatments with 7 replicates of 13 broilers each for 35\u2009d, namely: control treatment (NC) with no IQ addition in the diet and treatment (M) with dietary supplementation at 200\u2009mg\/kg diet of an IQs standardized blend. Broiler performance responses did not differ (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05) among the two treatments. The results showed that M birds had significantly higher (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) expression levels for antioxidant capacity, barrier integrity and inflammation\u2010related genes, at the duodenum compared to NC. Furthermore, at the cecal level, treatment M showed significant (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) downregulation of TLR (Toll\u2010like receptors) signaling and the subsequent inflammation components, compared to NC. Overall, under the optimal conditions of the trial, there were no significant growth performance benefits of IQs blend dietary addition in broilers diets. Nevertheless, IQs blend promoted gut homeostasis in 35\u2010days\u2010old broilers via the beneficial modulation of antioxidant and inflammatory responses, primarily at the duodenal level. The latter, under stress challenge conditions, may prove beneficial also for performance that needs to be specifically studied.\n                  <\/jats:p>","DOI":"10.1111\/jpn.70012","type":"journal-article","created":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T00:28:56Z","timestamp":1759883336000},"page":"1-12","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effects of an Isoquinoline Alkaloids Blend on the Expression of Genes Relevant for Antioxidant Capacity, Barrier Integrity and Inflammation Along the Broiler Gut"],"prefix":"10.1111","volume":"110","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3057-7073","authenticated-orcid":false,"given":"Vasileios V.","family":"Paraskeuas","sequence":"first","affiliation":[{"name":"Laboratory of Nutritional Physiology and Feeding, Department of Animal Science, School of Animal Biosciences Agricultural University of Athens Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0499-1564","authenticated-orcid":false,"given":"Ioannis","family":"Brouklogiannis","sequence":"additional","affiliation":[{"name":"Laboratory of Nutritional Physiology and Feeding, Department of Animal Science, School of Animal Biosciences Agricultural University of Athens Athens Greece"},{"name":"Feed Innovations &amp; Technologies P.C. A Spin\u2010Off Company of the Agricultural University of Athens Kifisia Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"Pastor","sequence":"additional","affiliation":[{"name":"Phytobiotics Futterzusatzstoffe GmbH, Eltville GermanyPhytobiotics Eltville Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7926-5604","authenticated-orcid":false,"given":"Konstantinos C.","family":"Mountzouris","sequence":"additional","affiliation":[{"name":"Laboratory of Nutritional Physiology and Feeding, Department of Animal Science, School of Animal Biosciences Agricultural University of Athens Athens Greece"},{"name":"Feed Innovations &amp; Technologies P.C. A Spin\u2010Off Company of the Agricultural University of Athens Kifisia Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2024.105241"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.676628"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2020.06.028"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40104-016-0079-7"},{"key":"e_1_2_8_6_1","first-page":"19","article-title":"Council Directive of 28 June 2007 Laying down Minimum Rules for the Protection of Chickens Kept for Meat Production","volume":"182","author":"EC, 43","year":"2007","journal-title":"Official Journal of the European Union"},{"key":"e_1_2_8_7_1","first-page":"33","article-title":"Directive of the European Parliament and of the Council of 22 September 2010 on the Protection of Animals 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