{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T03:42:15Z","timestamp":1792122135108,"version":"4.4.0"},"reference-count":67,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T00:00:00Z","timestamp":1760745600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T00:00:00Z","timestamp":1760745600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study was conducted to investigate the impacts of a low crude protein diet on laying performance, intestinal morphology, and tight junction protein gene expression in laying hens challenged with Salmonella enteritidis (\n                    <jats:italic>S. enteritidis<\/jats:italic>\n                    ). A total of 144 56\u2010week\u2010old Hy\u2010Line Brown laying hens were randomly allocated into two groups a nonchallenged group (CON) and an\n                    <jats:italic>S. enteritidis<\/jats:italic>\n                    \u2010challenged chicken group (SE, orally administrated 3\u2009ml\/hen, 10\n                    <jats:sup>9<\/jats:sup>\n                    CFU\n                    <jats:italic>S. enteritidis<\/jats:italic>\n                    ). After 7 days, the SE\u2010infected chickens were subjected to two dietary treatments. The experimental groups included a nonchallenged group fed a diet based on corn\u2010soybean meal (CON, 16.5% crude protein), an SE group fed a diet based on low crude protein (LCP, 13% crude protein) and an SE group fed a normal crude protein diet (NCP, 16.5% crude protein). The results showed that challenge with SE increased the plasma concentrations of lipopolysaccharide (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), decreased the relative mRNA expression of ZO\u20101 (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) in the jejunum. In addition, compared with CON, feeding a low\u2010protein diet significantly increased yolk colour, but there was no significant difference in Laying Performance, Plasma Immunoglobulin Levels, Plasma LPS Concentration and Jejunal Morphology and mRNA Expression of Intestinal Barrier Function. Sequencing data of 16S rDNA indicated that there was a tendency for the reduction of bacteroidetes after SE infection (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.06). Compared with the Con group, the Chao1 (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.08), Observed\u2010OTUs (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.08) and faith_pd (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.07) showed a decreasing trend in the LCP group. These results suggest that\n                    <jats:italic>S. enteritidis<\/jats:italic>\n                    infection of laying hens can cause intestinal mucosal damage, LCP can maintain the normal physiological function of laying hens, as well as the NCP group, which helps to save the use of protein feed raw materials.\n                  <\/jats:p>","DOI":"10.1111\/jpn.70017","type":"journal-article","created":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T19:41:01Z","timestamp":1760816461000},"page":"50-63","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of a Low Crude Protein Diet Supplemented With Crystalline Amino Acids on the Laying Performance and Intestinal Health of Laying Hens Challenged With Salmonella Enteritidis"],"prefix":"10.1111","volume":"110","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8518-2904","authenticated-orcid":false,"given":"Hui","family":"Liu","sequence":"first","affiliation":[{"name":"Key Laboratory of Efficient Utilization of Non\u2010grain Feed Resources (Co\u2010construction by Ministry and Province), Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, Department of Animal Science Shandong Agricultural University Taian Shandong Province China"},{"name":"Animal Husbandry and Veterinary Service Center of Xintai City Xintai Shandong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Tang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Efficient Utilization of Non\u2010grain Feed Resources (Co\u2010construction by Ministry and Province), Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, Department of Animal Science Shandong Agricultural 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Anthony","family":"Uyanga","sequence":"additional","affiliation":[{"name":"Key Laboratory of Efficient Utilization of Non\u2010grain Feed Resources (Co\u2010construction by Ministry and Province), Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, Department of Animal Science Shandong Agricultural University Taian Shandong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingfa","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Efficient Utilization of Non\u2010grain Feed Resources (Co\u2010construction by Ministry and Province), Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, Department of Animal Science Shandong Agricultural University Taian Shandong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuqi","family":"Yan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Efficient Utilization of Non\u2010grain Feed 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Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, Department of Animal Science Shandong Agricultural University Taian Shandong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumei","family":"Cai","sequence":"additional","affiliation":[{"name":"Key Laboratory of Efficient Utilization of Non\u2010grain Feed Resources (Co\u2010construction by Ministry and Province), Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, Department of Animal Science Shandong Agricultural University Taian Shandong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Liu","sequence":"additional","affiliation":[{"name":"Shandong Technology Innovation Center of Laying Hens Shandong Anchee Agricultural Animal Husbandry Science Technology Group Co. 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Jinan Shandong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0878-137X","authenticated-orcid":false,"given":"Hai","family":"Lin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Efficient Utilization of Non\u2010grain Feed Resources (Co\u2010construction by Ministry and Province), Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, Department of Animal Science Shandong Agricultural University Taian Shandong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongchao","family":"Jiao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Efficient Utilization of Non\u2010grain Feed Resources (Co\u2010construction by Ministry and Province), Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, Department of Animal Science Shandong Agricultural University Taian Shandong Province 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