{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T13:58:29Z","timestamp":1773928709299,"version":"3.50.1"},"reference-count":17,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T00:00:00Z","timestamp":1764288000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T00:00:00Z","timestamp":1764288000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Maximal lactate steady state (MLSS) and its predictive thresholds, including the lactate threshold (LT), are widely used to estimate endurance capacity, prescribe training, and assess the effects of training. The human literature identified approximately twenty\u2010five methods to predict the MLSS. This communication compares the concordance between the two D\n                    <jats:sub>MAX<\/jats:sub>\n                    methods and the MLSS. Under laboratory conditions, ten teaching horses underwent a submaximal graded exercise test (GXT) on a treadmill to obtain their lactate\u2010velocity curves (LVC). They performed three to five 30\u2010min continuous running sessions to determine the MLSS. The traditional D\n                    <jats:sub>MAX<\/jats:sub>\n                    method was determined using the maximal distance from LVC to the line formed by its endpoints. A new calculated D\n                    <jats:sub>MAX<\/jats:sub>\n                    method (D\n                    <jats:sub>MAXC<\/jats:sub>\n                    ) involved mathematically determining the point that results in the maximum distance from a curve connecting the start to the endpoint of the LVC. The agreement among the velocities corresponding to the D\n                    <jats:sub>MAX<\/jats:sub>\n                    (V\n                    <jats:sub>MAX<\/jats:sub>\n                    ), D\n                    <jats:sub>MAXC<\/jats:sub>\n                    \u00a0(V\n                    <jats:sub>MAXC<\/jats:sub>\n                    ), and the MLSS (V\n                    <jats:sub>MLSS<\/jats:sub>\n                    ) was established using a Bland\u2010Altman plot and ordinary least products (OLP) analysis. The average lactatemia at V\n                    <jats:sub>MLSS<\/jats:sub>\n                    , V\n                    <jats:sub>MAX<\/jats:sub>\n                    , and V\n                    <jats:sub>MAXC<\/jats:sub>\n                    was 1.50\u2009\u00b1\u20090.36\u2009mM, 2.02\u2009\u00b1\u20090.66\u2009mM, and 1.92\u2009\u00b1\u20090.65\u2009mM, with respective speeds of 5.42\u2009\u00b1\u20090.57\u2009m\/s, 6.42\u2009\u00b1\u20090.71\u2009m\/s, and 6.35\u2009\u00b1\u20090.66\u2009m\/s. The mean difference between V\n                    <jats:sub>MAX<\/jats:sub>\n                    \u2010V\n                    <jats:sub>MLSS<\/jats:sub>\n                    and V\n                    <jats:sub>MAXC<\/jats:sub>\n                    \u2010V\n                    <jats:sub>MLSS<\/jats:sub>\n                    was \u20101.01\u2009\u00b1\u20090.61 and \u20100.93\u2009\u00b1\u20090.67\u2009m\/s, respectively. V\n                    <jats:sub>MAX<\/jats:sub>\n                    and V\n                    <jats:sub>MAXC<\/jats:sub>\n                    did not show constant and proportional biases compared to V\n                    <jats:sub>MLSS<\/jats:sub>\n                    . In the GXT protocol used in this current study, D\n                    <jats:sub>MAX<\/jats:sub>\n                    approaches appear to be accurate and reliable methods for lactate threshold determination and estimating MLSS in teaching horses.\n                  <\/jats:p>","DOI":"10.1111\/jpn.70033","type":"journal-article","created":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T19:09:53Z","timestamp":1764356993000},"page":"218-224","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Maximal Lactate Steady State Prediction Using Two DMAX Methods in Horses Subjected to Treadmill\u2010Graded Exercise Test"],"prefix":"10.1111","volume":"110","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9180-8528","authenticated-orcid":false,"given":"Thayssa de Oliveira","family":"Littiere","sequence":"first","affiliation":[{"name":"Laboratory of Equine Exercise Physiology and Pharmacology (LAFEQ), Department of Morphology and Animal Physiology, Universidade Estadual Paulista (Unesp) Faculties of Agrarian and Veterinary Sciences, Jaboticabal S\u00e3o Paulo 14884\u2010900 Brazil"},{"name":"Federal University of Piau\u00ed (UFPI) Campus Professora Cinobelina Elvas (CPCE) Bom Jesus 64900\u2010000 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1773-4604","authenticated-orcid":false,"given":"Guilherme Barbosa","family":"da Costa","sequence":"additional","affiliation":[{"name":"Laboratory of Equine Exercise Physiology and Pharmacology (LAFEQ), Department of Morphology and Animal Physiology, Universidade Estadual Paulista (Unesp) Faculties of Agrarian and Veterinary Sciences, Jaboticabal S\u00e3o Paulo 14884\u2010900 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3412-1324","authenticated-orcid":false,"given":"Nathali Adrielli Agassi","family":"de Sales","sequence":"additional","affiliation":[{"name":"Laboratory of Equine Exercise Physiology and Pharmacology (LAFEQ), Department of Morphology and Animal Physiology, Universidade Estadual Paulista (Unesp) Faculties of Agrarian and Veterinary Sciences, Jaboticabal S\u00e3o Paulo 14884\u2010900 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6593-511X","authenticated-orcid":false,"given":"J\u00falia Ribeiro Garcia","family":"de Carvalho","sequence":"additional","affiliation":[{"name":"Laboratory of Equine Exercise Physiology and Pharmacology (LAFEQ), Department of Morphology and Animal Physiology, Universidade Estadual Paulista (Unesp) Faculties of Agrarian and Veterinary Sciences, Jaboticabal S\u00e3o Paulo 14884\u2010900 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7164-604X","authenticated-orcid":false,"given":"Ivan Dario Martinez","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Laboratory of Equine Exercise Physiology and Pharmacology (LAFEQ), Department of Morphology and Animal Physiology, Universidade Estadual Paulista (Unesp) Faculties of Agrarian and Veterinary Sciences, Jaboticabal S\u00e3o Paulo 14884\u2010900 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7748-7193","authenticated-orcid":false,"given":"Gabriel Vieira","family":"Ramos","sequence":"additional","affiliation":[{"name":"Laboratory of Equine Sports Medicine (LMEE), Department of Clinic and Veterinary Surgery, School of Agricultural and Veterinarian Sciences (FCAV) S\u00e3o Paulo State University (UNESP) Jaboticabal 14884\u2010900 S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5659-0404","authenticated-orcid":false,"given":"Jos\u00e9 Correa","family":"de Lacerda\u2010Neto","sequence":"additional","affiliation":[{"name":"Laboratory of Equine Sports Medicine (LMEE), Department of Clinic and Veterinary Surgery, School of Agricultural and Veterinarian Sciences (FCAV) S\u00e3o Paulo State University (UNESP) Jaboticabal 14884\u2010900 S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6463-6628","authenticated-orcid":false,"given":"Guilherme Camargo","family":"Ferraz","sequence":"additional","affiliation":[{"name":"Laboratory of Equine Exercise Physiology and Pharmacology (LAFEQ), Department of Morphology and Animal Physiology, Universidade Estadual Paulista (Unesp) Faculties of Agrarian and Veterinary Sciences, Jaboticabal S\u00e3o Paulo 14884\u2010900 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