{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T22:05:27Z","timestamp":1784153127552,"version":"3.55.0"},"reference-count":61,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100010677","name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria","doi-asserted-by":"publisher","award":["INTA; PD\u2010L01\u2010I118 and PD\u2010L01\u2010I130"],"award-info":[{"award-number":["INTA; PD\u2010L01\u2010I118 and PD\u2010L01\u2010I130"]}],"id":[{"id":"10.13039\/501100010677","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Omega\u20103 (\u03c9\u22123) polyunsaturated fatty acids are well recognized for their potential to influence metabolic adaptation in dairy cows during the transition period. This study evaluated the effects of calcium salts enriched with \u03c9\u22123 fatty acids, including \u03b1\u2010linolenic acid and long\u2010chain \u03c9\u22123 fatty acids such as eicosapentaenoic and docosahexaenoic acids, on hepatic lipid metabolism\u2010related proteins, oxidative stress biomarkers, and liver function in postpartum dairy cows. Prior to calving, cows received a total mixed ration supplemented with 0.400\u2009kg dry matter (DM)\/day of calcium salts derived from a flaxseed oil\u2013fish oil mixture (60:40). After calving, cows were fed a partial mixed ration and received 0.650\u2009kg DM\/day of the same supplement mixed with a commercial concentrate during milking. Control cows received isoenergetic diets in which calcium salts were replaced with ground corn. Blood, urine, and liver samples were collected at \u221221 days relative to calving (used as a baseline covariate) and at +7, and +21 (\u00b13) days postpartum, when treatment effects were evaluated. No differences were observed between treatments in conventional biochemical markers of liver function or hepatic triacylglycerol concentration. Plasma malondialdehyde concentrations were higher in \u03c9\u22123\u2010supplemented cows, whereas other oxidative stress parameters were not affected. Supplementation with \u03c9\u22123 fatty acids was associated with changes in the abundance of selected hepatic proteins involved in fatty acid metabolism, including increased acyl\u2010CoA oxidase 1 and 3\u2010hydroxy\u20103\u2010methylglutaryl\u2010CoA synthase and reduced diacylglycerol O\u2010acyltransferase 1 abundance, whereas carnitine palmitoyltransferase 1 and peroxisome proliferator\u2010activated receptor alpha abundance were not affected. These findings indicate that \u03c9\u22123\u2010enriched calcium salts induce molecular adjustments in hepatic lipid metabolism during early lactation without evidence of adverse effects on liver function.<\/jats:p>","DOI":"10.1111\/jpn.70062","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T07:11:01Z","timestamp":1777360261000},"page":"523-533","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dietary Omega\u20103 Fatty Acids Alter Hepatic Lipid Oxidation and Oxidative Stress Markers in Postpartum Dairy Cows"],"prefix":"10.1111","volume":"110","author":[{"given":"Gino","family":"Storani","sequence":"first","affiliation":[{"name":"Laboratorio de Biolog\u00eda Celular y Molecular Aplicada, Instituto de Ciencias Veterinarias del Litoral (ICiVet\u2010Litoral) Universidad Nacional del Litoral (UNL)\/Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), 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