{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T22:04:44Z","timestamp":1784153084623,"version":"3.55.0"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T00:00:00Z","timestamp":1778976000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T00:00:00Z","timestamp":1778976000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The pursuit of high milk yields via high\u2010concentrate diets risks ruminal acidosis and microbial dysbiosis, triggering lipopolysaccharide (LPS) release, subacute ruminal acidosis (SARA) incidence, and substantial economic losses. This study evaluated the efficacy of the dietary intestinal alkaline phosphatase (IAP) against high\u2010concentrate feeding effects, hypothesizing it prevents SARA by degrading LPS, modulating microbiota, stabilizing ruminal pH, and improving gut health. Thirty\u2010six lactating cows were stratified into three groups (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200912): a high\u2010concentrate diet group (HC, 5:5 concentrate:forage), an HC group supplemented with IAP (HC\u2009+\u2009IAP), and a low\u2010concentrate control group (LC, 3:7). Key biomarkers such as ruminal pH, ruminal and fecal LPS, inflammatory cytokines (IL\u20106, IL\u20101\u03b2), and acute\u2010phase proteins (SAA, LBP)\u2010\u2010were monitored at 30\u2010day intervals from calving through lactation. SARA status was defined by ruminal pH: &lt;\u20095.6 (SARA), 5.6\u20135.8 (at\u2010risk), and \u2265\u20095.8 (healthy). Fecal microbiome analysis was conducted on HC and HC\u2009+\u2009IAP cows at 90 days in milk (DIM). The HC group exhibited a high incidence of SARA, peaking between 90 and 120 DIM. In stark contrast, dietary supplementation with IAP (0.5\u2009kg per 1000\u2009kg of concentrate feed) effectively stabilized ruminal fluid pH, thereby completely preventing the incidence of SARA throughout the entire lactation period. This prophylactic effect was associated with significantly reduced systemic concentrations of SAA, LBP, IL\u20106, and IL\u20101\u03b2. Moreover, IAP treatment markedly enhanced gut microbial diversity and the relative abundance of beneficial bacteria. Therefore, IAP acted primarily in the rumen to detoxify LPS, thereby stabilizing pH and preventing the occurrence of SARA, while also entering the intestine to regulate the structure of gut microbiota, thereby maintaining the health of dairy cows under high\u2010concentrate feeding conditions.\n                  <\/jats:p>","DOI":"10.1111\/jpn.70073","type":"journal-article","created":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T19:10:11Z","timestamp":1779045011000},"page":"595-605","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Intestinal Alkaline Phosphatase Alleviates Ruminal Acidosis in Dairy Cows Fed High\u2010Concentrate Diets Improves Their Overall Health"],"prefix":"10.1111","volume":"110","author":[{"given":"Yang","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Northwest A&amp;F University Yangling China"},{"name":"Mianyang Habio Bioengineering Co. Ltd. Mianyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqian","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Northwest A&amp;F University Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuaidong","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Northwest A&amp;F University Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zekang","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Northwest A&amp;F University Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quan","family":"Wei","sequence":"additional","affiliation":[{"name":"Beijing Aokest Biotechnology Co. Ltd. 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