{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T15:39:59Z","timestamp":1789486799306,"version":"build-2803163510"},"reference-count":59,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T00:00:00Z","timestamp":1780790400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T00:00:00Z","timestamp":1780790400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Managing prolactin (PRL)\u2010mediated reproductive suppression is critical for maintaining egg production in commercial laying hens. L\u2010tyrosine (TYR), a precursor of dopamine (DA), may counteract age\u2010related reproductive decline by suppressing PRL, thereby sustaining productivity and egg quality in late\u2010phase laying hens. The present study evaluated the effects of dietary L\u2010tyrosine supplementation on laying performance, egg quality, reproductive hormone profiles, gene expression, and promoter CpG methylation in aged laying hens. A total of 180 Atak\u2010S laying hens (71 weeks old) were randomly assigned to three dietary treatments, each with six replicates of 10 hens, and were fed a basal diet without L\u2010tyrosine (control) or the basal diet supplemented with 0.5\u2009g\/kg (TYR0.5) or 1\u2009g\/kg (TYR1) L\u2010tyrosine for 12 weeks. Hens in the TYR1 group exhibited higher hen\u2010day egg production, improved feed conversion ratio, and significantly greater eggshell weight, shell thickness, Haugh unit, yolk index, and albumen index than the control group (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Dietary L\u2010tyrosine increased plasma DA and serum estradiol while reducing serum PRL (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). L\u2010tyrosine also upregulated the expression of key dopaminergic and reproductive signalling genes, with corresponding promoter hypomethylation (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), whereas PRL mRNA expression tended to decrease with concurrent promoter hypermethylation. A significant negative correlation was observed between target gene expression and promoter CpG methylation levels (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). These findings indicate that dietary L\u2010tyrosine modulates reproductive hormone profiles and molecular responses within reproductive signalling pathways, resulting in improved laying performance and egg quality. Therefore, L\u2010tyrosine may represent a promising nutritional intervention to sustain reproductive function and productivity in late\u2010phase laying hens.\n                  <\/jats:p>","DOI":"10.1111\/jpn.70078","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T05:57:37Z","timestamp":1780898257000},"page":"685-698","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dietary L\u2010Tyrosine Sustains Egg Production and Quality by Modulating Promoter CpG Methylation, Gene Expression, and Hormone Profiles in Reproductive Signalling Pathways of Aged Laying Hens"],"prefix":"10.1111","volume":"110","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6807-8870","authenticated-orcid":false,"given":"Hasan H\u00fcseyin","family":"\u0130p\u00e7ak","sequence":"first","affiliation":[{"name":"Department of Animal Science, Faculty of Agriculture Dicle University Diyarbak\u0131r T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40104-019-0396-8"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pex241"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.21608\/epsj.2018.23238"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3923\/ijps.2004.17.19"},{"key":"e_1_2_8_6_1","volume-title":"Official Methods of Analysis","author":"AOAC International","year":"2000"},{"key":"e_1_2_8_7_1","doi-asserted-by":"crossref","unstructured":"Asiamah C. 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