{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T15:39:59Z","timestamp":1789486799291,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100009591","name":"North Carolina Agricultural Foundation","doi-asserted-by":"publisher","award":["660101"],"award-info":[{"award-number":["660101"]}],"id":[{"id":"10.13039\/100009591","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["02893"],"award-info":[{"award-number":["02893"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The objective of this study was to investigate the effects of reduced dietary acid\u2010binding capacity (ABC) by acidifiers on the properties of digesta along the gastrointestinal tract of pigs. In Exp. 1, 144 pigs at 21\u2009d of age [6.5\u2009\u00b1\u20090.9\u2009kg body weight (BW)] were housed in pens (3 pigs\/pen). In Exp. 2, 540 pigs weaned at 21\u2009d of age (6.9\u2009\u00b1\u20090.9\u2009kg BW) were housed in pens (3 barrows and 3 gilts\/pen). In Exp. 1, the basal diet was supplemented with 0.25% bacitracin methylene disalicylate, 0.30 or 0.50% lactic acid\u2010formic acid blend, or 0.30% lactic acid\u2010formic acid\u2010benzoic acid blend. In Exp. 2, the basal diet was supplemented with 0.25% bacitracin methylene disalicylate, 0.25, 0.35, or 0.50% benzoic acid, or 0.30, 0.40, or 0.60% sodium benzoate. In both Exp. 1 and 2, pigs were fed diets in 3 phases: phase 1 (7\u2009d in both Exp. 1 and 2), phase 2 (14\u2009d in Exp. 1 and 17\u2009d in Exp. 2), and phase 3 (21\u2009d in Exp. 1 and 17\u2009d in Exp. 2), respectively. The range of ABC at pH 4 in diets was 256 to 300, 194 to 220, and 129 to 176\u2009meq\/kg in phases 1, 2, and 3 of Exp. 1, and 234 to 273, 214 to 247, and 152 to 197\u2009meq\/kg in phases 1, 2, and 3 of Exp. 2, respectively. On d 42 in Exp. 1 and d 41 in Exp. 2, one pig representing the median BW in each pen was euthanized, and digesta from the stomach, proximal jejunum, distal jejunum, and cecum were collected to analyze the properties of digesta. The pH of digesta in the stomach was 4.0, which was lower (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) than the pH of digesta in the proximal jejunum (6.0), distal jejunum (5.9), and cecum (5.5). The pH of digesta in the cecum was lower (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) than the pH of the digesta in the proximal and distal jejunum. The dry matter (DM) content in stomach digesta was 32.5%, which was higher (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) than the DM content of digesta in the proximal jejunum (14.9%), distal jejunum (14.4%), and cecum (14.3%). The ABC at pH 4 of nursery diets was not correlated with the ABC at pH 3 of freeze\u2010dried stomach digesta and pH of stomach digesta. However, the ABC\u20103 of freeze\u2010dried stomach digesta was positively correlated (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) with the DM content in stomach digesta (\n                    <jats:italic>r<\/jats:italic>\n                    \u2009=\u20090.57) and the pH of stomach digesta (\n                    <jats:italic>r<\/jats:italic>\n                    \u2009=\u20090.63) in nursery pigs. The DM content in stomach digesta was also positively correlated\n                    <jats:italic>(p<\/jats:italic>\n                    \u2009&lt;\u20090.05) with the pH of stomach digesta of nursery pigs (\n                    <jats:italic>r<\/jats:italic>\n                    \u2009=\u20090.54). In conclusion, reducing dietary ABC by 40 to 50\u2009meq\/kg using acidifiers was not sufficient to reduce ABC or pH of stomach digesta in nursery pigs. However, the DM content in stomach digesta was positively correlated with its ABC and pH indicating that a decrease in the DM content and ABC of stomach digesta, if influenced by diets, is a primary factor contributing to reducing digesta pH in the stomach of nursery pigs.\n                  <\/jats:p>","DOI":"10.1111\/jpn.70091","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T18:17:59Z","timestamp":1784225879000},"page":"806-812","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Alteration of Acid\u2010Binding Capacity of Various Nursery Pig Diets and Its Influence on Properties of Digesta Along the Gastrointestinal Tract"],"prefix":"10.1111","volume":"110","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5874-2328","authenticated-orcid":false,"given":"Hyunjun","family":"Choi","sequence":"first","affiliation":[{"name":"Department of Animal Science North Carolina State University Raleigh North Carolina United State of America"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4591-1943","authenticated-orcid":false,"given":"Sung Woo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Animal Science North Carolina State University Raleigh North Carolina United State of America"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,16]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1051\/rnd:19870507"},{"key":"e_1_2_11_3_1","volume-title":"Official Methods of Analysis of AOAC International","author":"AOAC","year":"2005"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsre.2004.09.003"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(99)00166-2"},{"key":"e_1_2_11_6_1","first-page":"243","article-title":"Agar Electrophoretic Demonstration of Proteolytic Activity in Extracts of the Pig Gastric Mucosa","volume":"21","author":"Bottger G.","year":"1974","journal-title":"Zentralblatt Veterinary Medicine"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/2049-1891-4-19"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9610(79)90019-9"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.applanim.2005.08.014"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19850113"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19760059"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-30932-6"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3552"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anifeedsci.2025.116242"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas1982.5561380x"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas1961.201114x"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.218"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.5713\/ab.21.0010"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781119583998.ch10"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19620056"},{"key":"e_1_2_11_21_1","first-page":"45","article-title":"Effect of Creep Feeding, Dietary Fumaric Acid and Level of Dairy Product in the Diet on Post\u2010Weaning Pig Performance","author":"Lawlor P. 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