{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T06:13:35Z","timestamp":1788502415240,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The present study was designed to evaluate the impact of graded levels of\n                    <jats:italic>L. siceraria<\/jats:italic>\n                    leaf powder (0.0%, 0.2%, 0.4%, and 0.6%) in the diet on growth traits, immune response, oxidative status, gene expression, and tissue histology of male Ossimi\u2002lambs. Twenty\u2010four intact male lambs (10\u201312\u2002months; 32.60\u2009\u00b1\u20091.5\u2009kg) were allotted randomly to four experimental groups (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096) and received the particular diets for 90 days. Blood was collected for evaluation of haematologic indices, immune responses, antioxidant enzyme activities, and liver function markers. At the end of the trial, histopathological examination and digestive enzyme activity testing were performed on intestinal tissues. The findings indicated a marked\u2002enhancement in growth performance, immune response, and antioxidant defence in the LS groups. In addition, the corresponding mRNAs of growth\u2010 and immunity\u2010related genes (\n                    <jats:italic>GH, IGF\u20101, GHR, TLR\u20107, IL\u20106<\/jats:italic>\n                    , and\n                    <jats:italic>IL\u20101\u03b2<\/jats:italic>\n                    ) were significantly upregulated. Morphometric examination of the\u2002intestine showed increased villus height, villus height\/crypt depth ratio, and goblet cell number, specifically, at the 0.6% supplementation level. Histopathology showed normal liver architecture alongside enhanced intestinal barrier function and splenic immune activity. Furthermore, the reduction in liver enzymes implies potential\u2002hepatoprotective activity. Overall, supplementing the diet with 0.6% LS leaf powder represents a novel, effective, and safe approach to enhancing the health and productive performance of Ossimi lambs.\n                  <\/jats:p>","DOI":"10.1111\/jpn.70101","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T05:49:25Z","timestamp":1788500965000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Modulatory Effects of\n                    <i>Lagenaria Siceraria<\/i>\n                    Leaf Powder Supplementation on Growth Performance, Antioxidant Defence, Gene Expression, Histology, and Enzyme Activity in Ossimi Lambs (\n                    <i>Ovis Aries<\/i>\n                    )"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4369-8221","authenticated-orcid":false,"given":"Yasmin M.","family":"Abd El\u2010Aziz","sequence":"first","affiliation":[{"name":"Department of Zoology, Faculty of Science Port Said University  Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menna H. E.","family":"Morsy","sequence":"additional","affiliation":[{"name":"Zoology Department, Faculty of Science Arish University El\u2010Arish Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman H.","family":"Rashwan","sequence":"additional","affiliation":[{"name":"Department of Veterinary Hygiene and Management, Faculty of Veterinary Medicine Cairo University Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ehab","family":"El\u2010Haroun","sequence":"additional","affiliation":[{"name":"Department of Integrative Agriculture, College of Agriculture and Veterinary Medicine United Arab Emirates University Al Ain Abu Dhabi United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1733-6892","authenticated-orcid":false,"given":"Moaheda E.H.","family":"Eissa","sequence":"additional","affiliation":[{"name":"Biotechnology Department, Fish Farming and Technology Institute Suez Canal University  Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7229-2175","authenticated-orcid":false,"given":"El\u2010Sayed Hemdan","family":"Eissa","sequence":"additional","affiliation":[{"name":"Fish Research Centre, Faculty of Environmental Agricultural Sciences Arish University El\u2010Arish Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basma M.","family":"Hendam","sequence":"additional","affiliation":[{"name":"Department of Animal Wealth Development, Faculty of Veterinary Medicine Mansoura University Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010025\u201017070\u20106"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2025.1635706"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201074410\u20108"},{"key":"e_1_2_13_5_1","article-title":"Effect of Plant Leaf Supplementation on Growth Performance in Lambs","volume":"204","author":"Abdelrahman M. 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