{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T14:56:59Z","timestamp":1785596219676,"version":"3.56.0"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Understanding how nutritional management and genetic background influence hepatic metabolism is important for improving the nutritional quality of bovine products in tropical production systems. This study evaluated breed\u2010associated differences under a common dietary regimen and diet\u2010related differences within the Nelore breed with respect to hepatic cholesterol, lipid\u2010soluble antioxidant vitamins, fatty acid composition and lipid quality indices. Thirty\u2010six intact male cattle were allocated to three experimental groups: Nelore fed a silage\u2013concentrate diet, crossbred (\u00bd Nelore\u2009\u00d7\u2009\u00bd Angus) fed the same diet, and Nelore fed a high\u2010grain diet. Hepatic cholesterol and antioxidant vitamins were determined by high\u2010performance liquid chromatography, whereas fatty acid composition was analysed by gas chromatography with flame ionisation detection. No significant differences were observed in hepatic cholesterol concentration for either the breed\u2010associated or diet\u2010related contrasts (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05). Breed\u2010associated differences were detected for \u03b2\u2010carotene, \u03b2\u2010tocopherol, \u03b3\u2010tocopherol and docosahexaenoic acid (22:6\n                    <jats:italic>n\u2010<\/jats:italic>\n                    3) (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Diet\u2010related differences significantly affected \u03b2\u2010carotene, tocopherols, tocotrienols, several individual fatty acids and lipid quality indices, including the\n                    <jats:italic>n\u2010<\/jats:italic>\n                    6\n                    <jats:italic>\/n\u2010<\/jats:italic>\n                    3 ratio and hypocholesterolaemic and hypercholesterolaemic fatty acid indices (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Principal component analysis showed clear separation of animals by dietary treatment, with \u03b2\u2010carotene, \u03b1\u2010tocopherol and tocotrienols contributing strongly to sample discrimination. Overall, diet\u2010related differences within the Nelore breed were associated with significant changes in a broader range of hepatic lipid and antioxidant variables, whereas breed\u2010associated differences under a common dietary regimen were observed for a more limited set of variables. These findings highlight the importance of nutritional management in modulating hepatic composition and support the use of liver biomarkers to assess metabolic responses in beef cattle.\n                  <\/jats:p>","DOI":"10.1111\/jpn.70102","type":"journal-article","created":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T14:02:30Z","timestamp":1785592950000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Diet\u2010Related Differences Predominate Over Breed\u2010Associated Differences in Hepatic Lipid Metabolism, Antioxidant Vitamins and Lipid Quality Indices Among Beef Cattle"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3321-5078","authenticated-orcid":false,"given":"La\u00edza de K\u00e1ssia Mendes da","family":"Concei\u00e7\u00e3o","sequence":"first","affiliation":[{"name":"Graduate Program in Animal Science\u2014PPGCAN (Federal University of Par\u00e1\u2014UFPA, Brazilian Agricultural Research Corporation\u2014Embrapa Federal Rural University of the Amazon\u2014UFRA) Castanhal Brazil"},{"name":"Federal Rural University of the Amazon (UFRA) Bel\u00e9m Brazil"},{"name":"CIISA\u2014Interdisciplinary Research Centre in Animal Health Faculty of Veterinary Medicine, University of Lisbon Lisbon Portugal"},{"name":"Faculdade de Medicina Veterin\u00e1ria Av. Universidade T\u00e9cnica Lisbon Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Science (AL4AnimalS) Faculty of Veterinary Medicine, University of Lisbon Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6441-4577","authenticated-orcid":false,"given":"Jos\u00e9 de Brito","family":"Louren\u00e7o\u2010J\u00fanior","sequence":"additional","affiliation":[{"name":"Graduate Program in Animal Science\u2014PPGCAN (Federal University of Par\u00e1\u2014UFPA, Brazilian Agricultural Research Corporation\u2014Embrapa Federal Rural University of the Amazon\u2014UFRA) Castanhal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6400-6925","authenticated-orcid":false,"given":"Jamile Andr\u00e9a Rodrigues da","family":"Silva","sequence":"additional","affiliation":[{"name":"Federal Rural University of the Amazon (UFRA) Bel\u00e9m Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0322-1863","authenticated-orcid":false,"given":"Leonel Ant\u00f3nio","family":"Joaquim","sequence":"additional","affiliation":[{"name":"Graduate Program in Animal Science\u2014PPGCAN (Federal University of Par\u00e1\u2014UFPA, Brazilian Agricultural Research Corporation\u2014Embrapa Federal Rural University of the Amazon\u2014UFRA) Castanhal Brazil"},{"name":"Institute of Agricultural Research of Mozambique (IIAM) Esta\u00e7\u00e3o Zoot\u00e9cnica de Ang\u00f3nia Ang\u00f3nia Mo\u00e7ambique"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Juc\u00e1","family":"Moraes","sequence":"additional","affiliation":[{"name":"Graduate Program in Animal Science\u2014PPGCAN (Federal University of Par\u00e1\u2014UFPA, Brazilian Agricultural Research Corporation\u2014Embrapa Federal Rural University of the Amazon\u2014UFRA) Castanhal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7874-4937","authenticated-orcid":false,"given":"Thomaz Cyro Guimar\u00e3es de Carvalho","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Federal University of Roraima (UFRR) Boa Vista Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9355-4461","authenticated-orcid":false,"given":"Joana In\u00eas","family":"Ferreira","sequence":"additional","affiliation":[{"name":"CIISA\u2014Interdisciplinary Research Centre in Animal Health Faculty of Veterinary Medicine, University of Lisbon Lisbon Portugal"},{"name":"Faculdade de Medicina Veterin\u00e1ria Av. Universidade T\u00e9cnica Lisbon Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Science (AL4AnimalS) Faculty of Veterinary Medicine, University of Lisbon Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9081-8070","authenticated-orcid":false,"given":"Ana Maria Ramos","family":"Fontes","sequence":"additional","affiliation":[{"name":"CIISA\u2014Interdisciplinary Research Centre in Animal Health Faculty of Veterinary Medicine, University of Lisbon Lisbon Portugal"},{"name":"Faculdade de Medicina Veterin\u00e1ria Av. Universidade T\u00e9cnica Lisbon Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Science (AL4AnimalS) Faculty of Veterinary Medicine, University of Lisbon Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4863-1605","authenticated-orcid":false,"given":"Maria Pinheiro","family":"Sp\u00ednola","sequence":"additional","affiliation":[{"name":"CIISA\u2014Interdisciplinary Research Centre in Animal Health Faculty of Veterinary Medicine, University of Lisbon Lisbon Portugal"},{"name":"Faculdade de Medicina Veterin\u00e1ria Av. Universidade T\u00e9cnica Lisbon Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Science (AL4AnimalS) Faculty of Veterinary Medicine, University of Lisbon Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c9rica Coutinho","family":"David","sequence":"additional","affiliation":[{"name":"Beef Cattle Nutrition Center\u2014CINPEC Paragominas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ant\u00f4nio Vinicius Correa","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Federal Rural University of the Amazon (UFRA) Bel\u00e9m Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno Cabral","family":"Soares","sequence":"additional","affiliation":[{"name":"Federal Rural University of the Amazon (UFRA) Paragominas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1032-5987","authenticated-orcid":false,"given":"Jos\u00e9 Ant\u00f3nio Mestre","family":"Prates","sequence":"additional","affiliation":[{"name":"CIISA\u2014Interdisciplinary Research Centre in Animal Health Faculty of Veterinary Medicine, University of Lisbon Lisbon Portugal"},{"name":"Faculdade de Medicina Veterin\u00e1ria Av. Universidade T\u00e9cnica Lisbon Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Science (AL4AnimalS) Faculty of Veterinary Medicine, University of Lisbon Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,1]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meatsci.2009.11.014"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/87559129.2015.1015139"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2009.04.064"},{"key":"e_1_2_11_5_1","volume-title":"Anais do XIV Congresso Brasileiro de Meteorologia","author":"Bastos T. X.","year":"2006"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40104-020-00512-8"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.4141\/cjas87-080"},{"key":"e_1_2_11_8_1","unstructured":"Brazil.2020. DECRETO N\u00b0 10.468 DE 18 DE AGOSTO DE 2020. Altera o Decreto n\u00b0 9.013 de 29 de mar\u00e7o de 2017 que regulamenta a Lei n\u00b0 1.283 de 18 de dezembro de 1950 e a Lei n\u00b0 7.889 de 23 de novembro de 1989 que disp\u00f5em sobre o regulamento da inspe\u00e7\u00e3o industrial e sanit\u00e1ria de produtos de origem animal. (Acesso em: 25 de outubro de 2025).https:\/\/www.planalto.gov.br\/ccivil_03\/_ato2019-2022\/2020\/decreto\/d10468.htm."},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(85)90299-7"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfca.2026.109209"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-10-65"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meatsci.2007.12.006"},{"key":"e_1_2_11_13_1","volume-title":"M\u00e9todos para an\u00e1lise de alimentos","author":"Detmann E.","year":"2012"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3746\/pnf.2024.29.1.18"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)64849-5"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meatsci.2009.02.002"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-021-02861-6"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41538-024-00315-5"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.5851\/kosfa.2014.34.2.214"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.2527\/1995.73103131x"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods13111595"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods12173226"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.33448\/rsd-v14i4.48595"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-25605-3"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anifeedsci.2006.06.018"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2019.12.048"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fanim.2024.1329346"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani14060840"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2005.01.021"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani13243785"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41538-025-00397-9"},{"key":"e_1_2_11_32_1","volume-title":"Bovinocultura: Manejo e alimenta\u00e7\u00e3o de bovinos de corte em confinamento (Cole\u00e7\u00e3o SENAR, No. 232)","author":"Servi\u00e7o Nacional de Aprendizagem Rural (SENAR)","year":"2018"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani12050595"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.2527\/1992.70113562x"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)91846-M"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-015-0360-6"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani15131856"}],"container-title":["Journal of Animal Physiology and Animal Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpn.70102","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jpn.70102","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpn.70102","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T14:02:33Z","timestamp":1785592953000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpn.70102"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,1]]},"references-count":36,"alternative-id":["10.1111\/jpn.70102"],"URL":"https:\/\/doi.org\/10.1111\/jpn.70102","archive":["Portico"],"relation":{},"ISSN":["0931-2439","1439-0396"],"issn-type":[{"value":"0931-2439","type":"print"},{"value":"1439-0396","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,1]]},"assertion":[{"value":"2025-11-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"jpn.70102"}}