{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T06:07:16Z","timestamp":1788934036159,"version":"build-2803163510"},"reference-count":72,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Physiology Nutrition"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study evaluated the effects of intra\u2010amniotic administration of combined energy, amino acid, and vitamin sources on embryonic development, post\u2010hatch growth performance, and intestinal health in broiler chickens. On Day 18 of incubation, 460 fertile Ross 308 eggs were allocated to four groups: non\u2010injected negative control (NC), saline\u2010injected positive control (PC), nutrient solution I (NS\u2010I; glucose, lysine, and ascorbic acid), and nutrient solution II (NS\u2010II; creatine monohydrate, glutamine, and ascorbic acid). At hatch, chick quality traits and intestinal histomorphometric and immunohistochemical parameters were assessed, and post\u2010hatch performance was monitored for 35 days. Intra\u2010amniotic administration, particularly NS\u2010II, significantly increased hatch weight, chick length, and total intestinal length (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Both nutrient solutions improved jejunal morphology at hatch by increasing villus height, the villus height\u2010to\u2010crypt depth ratio, and absorptive surface area, while reducing crypt depth (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). The proliferation index and PCNA expression were significantly higher in the nutrient\u2010injected groups, indicating enhanced epithelial cell proliferation. During the 35\u2010day rearing period, NS\u2010II significantly improved body weight gain, feed intake, carcass weight, and the European Production Efficiency Factor (EPEF) (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), without adversely affecting the feed conversion ratio. Cecal microbiota composition and overall meat quality parameters were not negatively affected; however, lower coliform and mesophilic bacterial counts were observed in meat samples from the NS\u2010II group during refrigerated storage on Days 5 9. In conclusion, intra\u2010amniotic supplementation with combined energy, amino acid, and vitamin sources, particularly creatine monohydrate and glutamine combined with ascorbic acid, positively modulated early intestinal development and improved growth performance in broilers without compromising meat quality.\n                  <\/jats:p>","DOI":"10.1111\/jpn.70111","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T05:48:02Z","timestamp":1788932882000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Combination of In Ovo Nutritional Components Improves Embryonic Development, Growth Performance, and Gut Health in Broiler Chickens"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1816-2684","authenticated-orcid":false,"given":"Derya","family":"Ye\u015filba\u011f","sequence":"first","affiliation":[{"name":"Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine Bursa Uluda\u011f University Bursa T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fulya Odaba\u015fi","family":"Erbay","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine Bursa Uluda\u011f University Bursa T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6468-8535","authenticated-orcid":false,"given":"Ender Deniz","family":"Asmaz","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology Istanbul Ayd\u0131n University \u0130stanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1053-6250","authenticated-orcid":false,"given":"Berrin","family":"Zik","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Veterinary Medicine Bursa Uluda\u011f University Bursa T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9510-5734","authenticated-orcid":false,"given":"Artun","family":"Yibar","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene and Technology, Faculty of Veterinary Medicine Bursa Uluda\u011f University Bursa T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilmi Can","family":"Acar","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine Bursa Uluda\u011f University Bursa T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Engin","family":"Erbay","sequence":"additional","affiliation":[{"name":"HasTavuk Inc. Bursa T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1793-6879","authenticated-orcid":false,"given":"Kadir","family":"Ye\u015filba\u011f","sequence":"additional","affiliation":[{"name":"Department of Virology, Faculty of Veterinary Medicine Bursa Uluda\u011f University Bursa T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,8]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"Ovo Techniques and Treatments in Poultry Eggs","author":"Alagawany M.","year":"2022"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2012.03.005"},{"key":"e_1_2_10_4_1","volume-title":"Food Mycrobiology and Applications","author":"Anonymous","year":"2000"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pez392"},{"key":"e_1_2_10_6_1","unstructured":"Aviagen.2022.Ross\u2010308 Performance Objectives.https:\/\/aviagen.com\/assets\/Tech_Center\/Ross_Broiler\/RossxRoss308-broilerPerformanceObjectives2022-EN.pdf."},{"issue":"1","key":"e_1_2_10_7_1","first-page":"217","article-title":"Donmu\u015f midyelerin bazi fiziksel, kimyasal ve mikrobiyolojik \u00f6zellikleri","volume":"20","author":"Bayizit A. 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