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This study combined in vivo and in vitro approaches to investigate associations between feed sorting behaviour, host metabolism, and rumen microbial fermentation. Twenty\u2010four mid\u2010lactation Holstein cows were monitored for 28 days, from which 12 cows representing extreme feed\u2010sorting phenotypes were selected: severe sorters for fine particles (SES;\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096) and slight sorters (SLS;\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096). Blood samples were collected for biochemical and metabolomic analyses, and pooled rumen fluid from selected cows within each sorting category was incubated in vitro with high\u2010concentrate or high\u2010forage substrates. Excessive sorting cows exhibited higher alanine aminotransferase, total cholesterol, and low\u2010density lipoprotein cholesterol concentrations. Metabolomic analysis revealed alterations in carbohydrate, lipid, and amino acid metabolism, including changes in\n                    <jats:sc>D<\/jats:sc>\n                    \u2010mannose, fumaric acid, mevalonic acid, oleic acid, glutamic acid, tryptophan, and gamma\u2010aminobutyric acid concentrations. These changes were accompanied by increased concentrations of PGE2, histamine, and arachidonoyl ethanolamide, consistent with altered inflammatory and endocannabinoid signalling. In vitro incubation of pooled SES\u2010 and SLS\u2010derived inocula with high\u2010concentrate and high\u2010forage substrates generated distinct fermentation profiles. Treatments containing SES\u2010derived inocula were characterised by lower pH and greater total volatile fatty acid concentrations, particularly under high\u2010concentrate conditions. These fermentation patterns were associated with higher relative abundances of\n                    <jats:italic>Succinivibrionaceae UCG<\/jats:italic>\n                    \u2212\n                    <jats:italic>002<\/jats:italic>\n                    ,\n                    <jats:italic>Lachnobacterium<\/jats:italic>\n                    , and\n                    <jats:italic>Pseudobutyrivibrio<\/jats:italic>\n                    . Overall, these findings suggest that feed sorting behaviour is associated with alterations in systemic metabolism and rumen microbial fermentation characteristics, providing new insight into the relationship between feeding behaviour and metabolic health in dairy cows.\n                  <\/jats:p>","DOI":"10.1111\/jpn.70119","type":"journal-article","created":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T17:35:46Z","timestamp":1791740146000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Excessive Feed Sorting Is Associated With Altered Plasma Metabolism and Distinct Fermentation Responses of Pooled Rumen Microbiota in Dairy Cows"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3043-5358","authenticated-orcid":false,"given":"Abdallah A.","family":"Mousa","sequence":"first","affiliation":[{"name":"Innovation Center for Ruminant Nutrition and Cleaner Production, College of Animal 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