{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:27:48Z","timestamp":1787239668323,"version":"build-2736575974"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,6,21]],"date-time":"2019-06-21T00:00:00Z","timestamp":1561075200000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Phycology"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:p>\n                    The two most toxic\n                    <jats:italic>Gambierdiscus<\/jats:italic>\n                    species identified from the Caribbean are\n                    <jats:italic>G.\u00a0excentricus<\/jats:italic>\n                    and\n                    <jats:italic>G.\u00a0silvae<\/jats:italic>\n                    . These species are the primary causes of ciguatera fish poisoning and likely contribute disproportionately to the toxicity of marine food webs. While\n                    <jats:italic>Gambierdiscus<\/jats:italic>\n                    species are difficult to distinguish using light or scanning electron microscopy, reliable species\u2010specific molecular identification methods have been developed and used successfully to identify a number of other\n                    <jats:italic>Gambierdiscus<\/jats:italic>\n                    species. Corresponding species\u2010specific assays are not yet available for\n                    <jats:italic>G.\u00a0excentricus<\/jats:italic>\n                    and\n                    <jats:italic>G.\u00a0silvae<\/jats:italic>\n                    , which imposes limitations on species identification and related ecological studies. The following note describes species\u2010specific polymerase chain reaction assays for\n                    <jats:italic>G.\u00a0excentricus<\/jats:italic>\n                    and\n                    <jats:italic>G.\u00a0silvae<\/jats:italic>\n                    that can be used for these purposes.\n                  <\/jats:p>","DOI":"10.1111\/jpy.12852","type":"journal-article","created":{"date-parts":[[2019,2,28]],"date-time":"2019-02-28T11:59:37Z","timestamp":1551355177000},"page":"730-732","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Species\u2010specific\n                    <scp>PCR<\/scp>\n                    assays for\n                    <i>Gambierdiscus excentricus<\/i>\n                    and\n                    <i>Gambierdiscus silvae<\/i>\n                    (Gonyaulacales, Dinophyceae)"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6293-8000","authenticated-orcid":false,"given":"R. Wayne","family":"Litaker","sequence":"first","affiliation":[{"name":"National Oceanic and Atmospheric Administration National Ocean Service National Centers for Coastal Ocean Science Beaufort Laboratory 101 Pivers Island Rd. Beaufort North Carolina 28516 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia A.","family":"Tester","sequence":"additional","affiliation":[{"name":"Ocean Tester, LLC 295 Dills Point Road Beaufort North Carolina 28516 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark W.","family":"Vandersea","sequence":"additional","affiliation":[{"name":"National Oceanic and Atmospheric Administration National Ocean Service National Centers for Coastal Ocean Science Beaufort Laboratory 101 Pivers Island Rd. Beaufort North Carolina 28516 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,21]]},"reference":[{"key":"e_1_2_3_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2009.06.013"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins10010002"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.protis.2014.09.003"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hal.2011.06.013"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/md15030072"},{"key":"e_1_2_3_7_1","first-page":"19","article-title":"First report of a mass\u2010poisoning outbreak following the consumption of Tectus niloticus (Gastropod) in French Polynesia: a novel pathway of ciguatera shellfish poisoning?","volume":"50","author":"Gatti C.","year":"2015","journal-title":"Harmful Algae News"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(00)00161-6"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-007-6419-4_13"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0185776"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.2216\/07-15.1"},{"key":"e_1_2_3_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2010.05.017"},{"key":"e_1_2_3_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hal.2015.11.018"},{"key":"e_1_2_3_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0060882"},{"key":"e_1_2_3_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hal.2017.02.005"},{"key":"e_1_2_3_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10811-018-1456-8"},{"key":"e_1_2_3_17_1","unstructured":"Robertson A. Richlen M. L. Erdner D. Smith T. B. Anderson D. M. Liefer J. Xu Y. McCarron P. Miles C.&Parsons M. L.2018.Toxicity chemistry and implications of Gamberdiscus silvae: A ciguatoxin superbug in the Greater Caribbean region. International Conference on Harmful Alage Nantes France. 21\u201326 October 2018. 115\u00a0pp."},{"key":"e_1_2_3_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/md15080243"},{"key":"e_1_2_3_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2010.02.026"},{"key":"e_1_2_3_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1529-8817.2012.01146.x"},{"key":"e_1_2_3_21_1","doi-asserted-by":"publisher","DOI":"10.2216\/16-41.1"}],"container-title":["Journal of Phycology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjpy.12852","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpy.12852","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jpy.12852","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpy.12852","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T09:38:26Z","timestamp":1694252306000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpy.12852"}},"subtitle":[],"editor":[{"given":"S.","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,6]]},"references-count":21,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,6]]}},"alternative-id":["10.1111\/jpy.12852"],"URL":"https:\/\/doi.org\/10.1111\/jpy.12852","archive":["Portico"],"relation":{},"ISSN":["0022-3646","1529-8817"],"issn-type":[{"value":"0022-3646","type":"print"},{"value":"1529-8817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6]]},"assertion":[{"value":"2018-03-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}