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The growth of <jats:italic>Crocosphaera<\/jats:italic> can be limited by low phosphorus (P) concentrations in these oligotrophic environments. Biomarkers such as the high\u2010affinity <jats:styled-content style=\"fixed-case\">ABC<\/jats:styled-content> transporter phosphate\u2010binding gene, <jats:italic>pstS<\/jats:italic>, are commonly used to monitor when such organisms are under P stress; however, transcriptional regulation of these markers is often complex and not well\u2010understood. In this study, we interrogated changes in <jats:italic>pstS<\/jats:italic> transcript levels in <jats:italic>C.\u00a0watsonii<\/jats:italic> cells under P starvation, and in response to added dissolved inorganic phosphorus (<jats:styled-content style=\"fixed-case\">DIP<\/jats:styled-content>), dissolved organic phosphorus (<jats:styled-content style=\"fixed-case\">DOP<\/jats:styled-content>), and changing light levels. We observed elevated relative <jats:italic>pstS<\/jats:italic> transcript levels in <jats:italic>C.\u00a0watsonii <\/jats:italic><jats:styled-content style=\"fixed-case\">WH<\/jats:styled-content>8501 at <jats:styled-content style=\"fixed-case\">DIP<\/jats:styled-content> concentrations below 60 and above 20\u00a0nmol\u00a0\u00b7\u00a0L<jats:sup>\u22121<\/jats:sup>. Transcript levels were suppressed by both inorganic and bioavailable organic phosphorus; however, the P stress response was more sensitive to <jats:styled-content style=\"fixed-case\">DIP<\/jats:styled-content> than <jats:styled-content style=\"fixed-case\">DOP<\/jats:styled-content> sources. Increasing light intensity resulted in increased relative <jats:italic>pstS<\/jats:italic> transcript abundances independently of low external P, and seemed to exacerbate the physiological effects of P stress. The variable response to different P compounds and rapid and transient influence of high light on <jats:italic>pstS<\/jats:italic> transcript abundances suggests that <jats:italic>pstS<\/jats:italic> is an indicator of internal P status in <jats:italic>Crocosphaera<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/jpy.12863","type":"journal-article","created":{"date-parts":[[2019,3,31]],"date-time":"2019-03-31T07:34:21Z","timestamp":1554017661000},"page":"752-761","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Use of the high\u2010affinity phosphate transporter gene, <i>pstS<\/i>, as an indicator for phosphorus stress in the marine diazotroph <i>Crocosphaera watsonii<\/i> (<i>Chroococcales<\/i>,<i> Cyanobacteria<\/i>)"],"prefix":"10.1111","volume":"55","author":[{"given":"Nicole","family":"Pereira","sequence":"first","affiliation":[{"name":"Ocean Sciences Department University of California 1156 High Street Santa Cruz California 95064 USA"},{"name":"Second Genome 341 Allerton Avenue South San Francisco California 94080 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina N.","family":"Shilova","sequence":"additional","affiliation":[{"name":"Ocean Sciences Department University of California 1156 High Street Santa Cruz California 95064 USA"},{"name":"Second Genome 341 Allerton Avenue South San Francisco California 94080 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5691-5408","authenticated-orcid":false,"given":"Jonathan P.","family":"Zehr","sequence":"additional","affiliation":[{"name":"Ocean Sciences Department University of California 1156 High Street Santa Cruz California 95064 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5,16]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1994.tb00399.x"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M312202200"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00001342"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpy.12090"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.2003.48.5.1721"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.20.5692-5699.2000"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.2003.48.3.1049"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3354\/ame022185"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0967-0637(01)00027-9"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/30881"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2006.00180.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3354\/meps09297"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2011.02675.x"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.2.1452-1458.2006"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.2006.51.3.1381"},{"key":"e_1_2_8_16_1","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1515\/9781400849727","volume-title":"Aquatic Photosynthesis","author":"Falkowski P. 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