{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T00:09:19Z","timestamp":1792282159393,"version":"4.4.0"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T00:00:00Z","timestamp":1763164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["42176100"],"award-info":[{"award-number":["42176100"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010038","name":"Earmarked Fund for China Agriculture Research System","doi-asserted-by":"publisher","award":["CARS\u201050"],"award-info":[{"award-number":["CARS\u201050"]}],"id":[{"id":"10.13039\/501100010038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Phycology"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Undaria pinnatifida<\/jats:italic>\n                    is an economically important kelp that is extensively farmed in East Asia. Increasingly severe ocean warming makes it urgent to breed new cultivars that are resistant to higher temperatures. Breeding of triploid sporophytes is a potential way to achieve this purpose. Diploid gametophytes were previously produced via apospory from heterozygous sporophytes; however, their sex was not pure, making it difficult to conduct a precise cross. In the present study, we addressed this issue by means of obtaining aposporous diploid gametophytes from doubled haploid sporophytes that were produced by self\u2010fertilization of a monoicous gametophyte. The sex of the aposporous diploid gametophytes was determined to be male. We crossed the male diploid gametophytes with three female haploid gametophyte clonal lines and obtained three hybrid sporophyte lines, which were identified to be triploid using flow cytometry analysis. We cultivated the three triploid lines in the open sea and evaluated their economic traits in April. Their total length and blade length were significantly higher than the control cultivar despite their cultivation period being ~2\u2009months shorter than that of the control. Obvious decay was observed in the control, but not in the triploid sporophytes, suggesting that they sustained growth in higher temperatures. The triploid sporophytes formed sporophylls but were not able to release spores in the reproductive season, and thus, they were determined to be sterile. This study provides a novel and precise means for breeding triploid sporophytes in\n                    <jats:italic>U. pinnatifida<\/jats:italic>\n                    , which is expected to be useful for breeding triploid cultivars superior to the current diploid cultivars.\n                  <\/jats:p>","DOI":"10.1111\/jpy.70107","type":"journal-article","created":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T09:16:14Z","timestamp":1763198174000},"page":"1874-1883","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Breeding of triploid\n                    <i>Undaria pinnatifida<\/i>\n                    through crossing aposporous gametophytes derived from doubled haploid sporophytes with haploid gametophytes"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3117-8365","authenticated-orcid":false,"given":"Tifeng","family":"Shan","sequence":"first","affiliation":[{"name":"State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Oceanology Chinese Academy of Sciences  Qingdao China"},{"name":"Laboratory for Marine Biology and Biotechnology Qingdao Marine Science and Technology Center  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqian","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Oceanology Chinese Academy of Sciences  Qingdao China"},{"name":"Laboratory for Marine Biology and Biotechnology Qingdao Marine Science and Technology Center  Qingdao China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2014.07.042"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1529-8817.2001.037003411.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2018.06.005"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/icesjms\/fsaa069"},{"key":"e_1_2_9_6_1","unstructured":"Food and Agriculture Organization of the United Nations. (2025).FishStatJ \u2010 Software for Fishery and Aquaculture Statistical Time Series \u2010 Fisheries and Aquaculture.https:\/\/www.fao.org\/fishery\/en\/statistics\/software\/fishstatj"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1529\u20108817.1980.tb03062.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/09670269600651601"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmars.2020.00110"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/00318884.2019.1639436"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00343\u2010014\u20103139\u2010x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10811\u2010019\u201001917\u20103"},{"key":"e_1_2_9_13_1","unstructured":"Li X. L.(2008).Study on polyploid and cross breeding of Undaria pinnatifida and Laminaria japonica[in Chinese with English abstract; Doctoral dissertation Ocean University of China]."},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2013.02.047"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00347797"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2019.03.002"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/jwas.12065"},{"key":"e_1_2_9_18_1","first-page":"63","volume-title":"Cultures and collections of algae","author":"Provasoli L.","year":"1968"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11240\u2010025\u201003011\u20108"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpy.13295"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2021.801937"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpy.13153"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440\u20101835.2008.00519.x"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10811\u2010015\u20100748\u20105"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.marenvres.2017.09.015"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2025.742491"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10811\u2010024\u201003351\u20106"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2014.06.003"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00004026"},{"key":"e_1_2_9_30_1","first-page":"45","article-title":"Polyploid breeding on vegetative cell of young sporophyte in Undaria pinnatifida","volume":"6","author":"Zhang Z. 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