{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T19:26:49Z","timestamp":1787081209792,"version":"build-2736575974"},"reference-count":57,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Phycology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Klebsormidium<\/jats:italic>\n                    and\n                    <jats:italic>Interfilum<\/jats:italic>\n                    are two closely related genera in the charophyte group Klebsormidiophyceae. Members of\n                    <jats:italic>Klebsormidium<\/jats:italic>\n                    are usually multicellular whereas those of\n                    <jats:italic>Interfilum<\/jats:italic>\n                    are unicellular. The genome of\n                    <jats:italic>K. nitens<\/jats:italic>\n                    has been sequenced using second\u2010generation sequencing technology, but the assembled scaffolds are frequently fragmented. In this study, we re\u2010sequenced the genomes of\n                    <jats:italic>K. nitens<\/jats:italic>\n                    using PacBio platform and obtained 15.8\u2009Gb long reads. We also sequenced the genome of\n                    <jats:italic>I. paradoxum<\/jats:italic>\n                    and obtained 13 Gb long reads. The\n                    <jats:italic>I. paradoxum<\/jats:italic>\n                    genome assembly (81.7\u2009Mb) included 59 contigs with N50 of 1.9\u2009Mb, and the\n                    <jats:italic>K. nitens<\/jats:italic>\n                    assembly (132.9\u2009Mb) included 188 contigs with N50 of 2.1\u2009Mb. A total of 20,425 and 15,103 protein\u2010coding genes were annotated in\n                    <jats:italic>K. nitens<\/jats:italic>\n                    and\n                    <jats:italic>I. paradoxum<\/jats:italic>\n                    , respectively. Comparative genome analyses identified 2805 gene families specific to Klebsormidiophyceae, which are enriched in gene ontology (GO) categories related to jasmonic acid (JA) biosynthesis and rRNA catabolism. Twenty\u2010seven gene families, enriched in RNA\u2013DNA hybrid ribonuclease activity and transmitter\u2010gated ion channel activity, were found to have expanded in the last common ancestor of\n                    <jats:italic>I. paradoxum<\/jats:italic>\n                    and\n                    <jats:italic>K. nitens<\/jats:italic>\n                    . Several genes related to cell wall structure, including galactosyltransferase (GALT), were absent in\n                    <jats:italic>I. paradoxum<\/jats:italic>\n                    . We also found that approximately 50% of abiotic stress\u2010related genes (e.g., late embryogenesis abundant,\n                    <jats:italic>LEA<\/jats:italic>\n                    genes) are present in land plants. The generation of\n                    <jats:italic>K. nitens<\/jats:italic>\n                    and\n                    <jats:italic>I. paradoxum<\/jats:italic>\n                    genomes and transcriptome data provides additional resources to understand the origin and evolution of land plants.\n                  <\/jats:p>","DOI":"10.1111\/jpy.70199","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T10:11:32Z","timestamp":1782209492000},"page":"1251-1263","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Klebsormidiophyceae sister genomes reveal multicellularity and stress adaptations"],"prefix":"10.1111","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6768-3107","authenticated-orcid":false,"given":"Jianchao","family":"Ma","sequence":"first","affiliation":[{"name":"State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, 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