{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T08:53:34Z","timestamp":1791881614286,"version":"4.3.4"},"reference-count":65,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["OPERA, JPMJOP1613"],"award-info":[{"award-number":["OPERA, JPMJOP1613"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["COI\u2010NEXT, JPMJPF2010"],"award-info":[{"award-number":["COI\u2010NEXT, JPMJPF2010"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Phycology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Microalgae accumulate triacylglycerols (TAGs) as storage lipids in response to nutrient limitation, yet the mechanisms governing TAG turnover remain incompletely understood. In the oleaginous microalga\n                    <jats:italic>Nannochloropsis oceanica<\/jats:italic>\n                    , functional studies of TAG degradation have focused on lipases containing patatin domains, whereas the roles of other lipase families remain poorly characterized. To address this shortcoming, we focused on class 3 lipases, a group distinct from patatin\u2010domain TAG lipases, and examined their roles in TAG metabolism in\n                    <jats:italic>N. oceanica<\/jats:italic>\n                    . Based on genome sequencing information and domain\u2010based screening, 23 putative class 3 lipases were identified and classified into two major orthogroups, including a lineage\u2010specific group unique to\n                    <jats:italic>Nannochloropsis<\/jats:italic>\n                    . Transcriptomic data obtained under nutrient\u2010deficient conditions were used to prioritize candidate genes for functional analysis. Targeted gene\u2010disruption mutants were examined under phosphate\u2010deficient conditions, which induce TAG accumulation while causing only relatively mild growth inhibition. Disruption of the\n                    <jats:italic>No3LIP6<\/jats:italic>\n                    or\n                    <jats:italic>No3LIP14<\/jats:italic>\n                    gene was consistently associated with increased TAG accumulation without severe growth defects, whereas other class 3 lipase mutants exhibited growth\u2010linked or condition\u2010dependent effects on TAG accumulation. In addition, disruption of selected class 3 lipases resulted in opposing changes in TAG\u2010associated fatty\u2010acid composition, suggesting differential contributions to TAG remodeling rather than uniform effects on TAG hydrolysis. Together, these results indicate that TAG turnover in\n                    <jats:italic>N. oceanica<\/jats:italic>\n                    grown under phosphate\u2010deficient conditions is associated with lipases beyond the patatin\u2010domain family, with a specific subset of class 3 lipases implicated in this process. This study highlights the functional relevance of non\u2010patatin TAG lipases in microalgal TAG metabolism.\n                  <\/jats:p>","DOI":"10.1111\/jpy.70203","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T10:49:39Z","timestamp":1783594179000},"page":"1478-1493","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification and functional analysis of class 3 lipases associated with triacylglycerol metabolism in\n                    <i>Nannochloropsis oceanica<\/i>\n                    (Eustigmatophyceae)"],"prefix":"10.1111","volume":"62","author":[{"given":"Masako","family":"Iwai","sequence":"first","affiliation":[{"name":"Phytolipid Technologies Co. Ltd.  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