{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T14:00:24Z","timestamp":1790258424136,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["DP200101613"],"award-info":[{"award-number":["DP200101613"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["217900"],"award-info":[{"award-number":["217900"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001138","name":"Australian Biological Resources Study","doi-asserted-by":"publisher","award":["4\u2010G046WSD"],"award-info":[{"award-number":["4\u2010G046WSD"]}],"id":[{"id":"10.13039\/501100001138","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Phycology"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Coral skeletons host diverse microbial communities, including endolithic green algae that contribute to holobiont functioning. The siphonous green alga\n                    <jats:italic>Ostreobium<\/jats:italic>\n                    (Bryopsidales) is arguably the best\u2010known coral endolith, but recent environmental sequencing has indicated that other lineages of green algae may also inhabit the coral skeleton, including members of the Cladophorales. Here, we characterize a new endolithic green alga isolated from a\n                    <jats:italic>Porites<\/jats:italic>\n                    coral skeleton collected from Heron Island on the southern Great Barrier Reef. The alga forms uniseriate, sparsely branched grass\u2010green filaments composed of elongated vegetative cells with infrequent cross walls. Phylogenetic analyses of 18S and 28S nrDNA gene sequences placed the strain as an early\u2010diverging lineage within the\n                    <jats:italic>Siphonocladus<\/jats:italic>\n                    clade of the Cladophorales order of the Ulvophyceae. Based on morphological and molecular evidence, we describe the alga as the new genus and species\n                    <jats:italic>Lithochloron capricornicum<\/jats:italic>\n                    . Analysis of environmental 18S nrDNA gene amplicon data from Great Barrier Reef corals revealed extensive sequence diversity, suggesting the genus has additional yet undescribed species. Environmental sequencing further showed that it is most abundant at intermediate depths within the coral skeleton and suggested niche differentiation with\n                    <jats:italic>Ostreobium<\/jats:italic>\n                    along the vertical light gradient in the skeleton, with\n                    <jats:italic>Lithochloron<\/jats:italic>\n                    tending to peak at shallower depths than\n                    <jats:italic>Ostreobium<\/jats:italic>\n                    . The discovery of\n                    <jats:italic>Lithochloron<\/jats:italic>\n                    challenges the long\u2010standing assumption that\n                    <jats:italic>Ostreobium<\/jats:italic>\n                    is the only dominant green algal endolith in coral skeletons and highlights the underestimated diversity of endolithic algae more broadly.\n                  <\/jats:p>","DOI":"10.1111\/jpy.70236","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T13:14:06Z","timestamp":1790255646000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Lithochloron<\/i>\n                    , a new genus of green algae residing in coral skeleton (Cladophorales, Chlorophyta)"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6305-4749","authenticated-orcid":false,"given":"Heroen","family":"Verbruggen","sequence":"first","affiliation":[{"name":"Spanish Institute of Oceanography, Spanish National Research Council (CSIC\u2010IEO), Joint Unit UPV\u2010IEO  Gandia Spain"},{"name":"Melbourne Integrative Genomics and School of BioSciences University of Melbourne  Parkville Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juntong","family":"Hu","sequence":"additional","affiliation":[{"name":"Melbourne Integrative Genomics and School of BioSciences University of Melbourne  Parkville Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqun","family":"Du","sequence":"additional","affiliation":[{"name":"Melbourne Integrative Genomics and School of BioSciences University of Melbourne  Parkville Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Wetherbee","sequence":"additional","affiliation":[{"name":"Melbourne Integrative Genomics and School of BioSciences University of Melbourne  Parkville Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Ricci","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Biomedicine Discovery Institute Monash University  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Poretti","sequence":"additional","affiliation":[{"name":"Melbourne Integrative Genomics and School of BioSciences University of Melbourne  Parkville Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010020\u201001056\u20105"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpy.70194"},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","unstructured":"delCampo J. Bonacolta A. M. Weiler B. A. Knowles B. Apprill A. Fox M. D. Wakeman K. C. Vermeij M. J. A. Rohwer F. &Keeling P. J.(2026).Global diversity and distribution of coral\u2010associated protists. bioRxiv.https:\/\/doi.org\/10.64898\/2026.01.22.700988","DOI":"10.64898\/2026.01.22.700988"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2016.101"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00060\u201021"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00397273"},{"key":"e_1_2_9_8_1","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/978-1-4615-8714-9_3","volume-title":"Culture of marine invertebrate animals","author":"Guillard R. R. L.","year":"1975"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1139\/m62\u2010029"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00338\u2010011\u20100750\u20106"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.2307\/1539860"},{"key":"e_1_2_9_12_1","first-page":"265","article-title":"Contributions \u00e0 la connaissance des Chaetophor\u00e9es epiphytes et endophytes et de leurs affinit\u00e9s","volume":"16","author":"Huber J.","year":"1893","journal-title":"Annales Des Sciences Naturelles, Botanique"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2021.01.018"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4285"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst010"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01984034"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1529\u20108817.2008.00506.x"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msae263"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248\u2010011\u20109946\u20100"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2018.07.008"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpy.13230"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2009.06.004"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1529\u20108817.1974.tb02722.x"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3354\/meps332119"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2017.164"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep31508"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201020196\u20105"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462\u20102920.15256"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01322636"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msaa015"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.2307\/1297526"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1080\/00318884.2022.2064132"},{"key":"e_1_2_9_33_1","doi-asserted-by":"crossref","unstructured":"Pasella M. M. Poretti M. Sim A. Ricci F. Powrie F. &Verbruggen H.(2026).Light\u2010harvesting strategies and competition drive niche partitioning amongOstreobiumlineages in the spectral architecture of the coral reef. bioRxiv.https:\/\/doi.org\/10.64898\/2026.03.02.709049","DOI":"10.64898\/2026.03.02.709049"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00341"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41396\u2010019\u20100548\u2010z"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/sysbio\/syy032"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00338\u2010021\u201002068\u2010z"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462\u20102920.16369"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3354\/meps243001"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/sysbio\/sys029"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1635"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12898\u2010016\u20100068\u2010x"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btab096"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmarsys.2025.104046"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168\u2010026\u201002414\u20109"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2022.03.010"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/giac127"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.176"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248\u2010007\u20109302\u20106"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579\u2010019\u20100223\u20104"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpy.70046"},{"issue":"6","key":"e_1_2_9_52_1","doi-asserted-by":"crossref","first-page":"471","DOI":"10.2216\/i0031-8884-39-6-471.1","article-title":"Phylogeny of the Bryopsidaceae (Bryopsidales, Chlorophyta): Cladistic analyses of morphological and molecular data","volume":"39","author":"Woolcott G. W.","year":"2000","journal-title":"Phycologia"}],"container-title":["Journal of Phycology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpy.70236","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jpy.70236","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jpy.70236","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T13:14:12Z","timestamp":1790255652000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpy.70236"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,24]]},"references-count":51,"alternative-id":["10.1111\/jpy.70236"],"URL":"https:\/\/doi.org\/10.1111\/jpy.70236","archive":["Portico"],"relation":{},"ISSN":["0022-3646","1529-8817"],"issn-type":[{"value":"0022-3646","type":"print"},{"value":"1529-8817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,24]]},"assertion":[{"value":"2026-06-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"jpy.70236"}}