{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T12:59:31Z","timestamp":1789822771264,"version":"4.0.1"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,9,14]],"date-time":"2012-09-14T00:00:00Z","timestamp":1347580800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Some clinical cases of hypoplastic tooth root are congenital. Because the formation of <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>ertwig's epithelial root sheath (<jats:styled-content style=\"fixed-case\">HERS<\/jats:styled-content>) is an important event for root development and growth, we have considered that understanding the <jats:styled-content style=\"fixed-case\">HERS<\/jats:styled-content> developmental mechanism contributes to elucidate the causal factors of the disease. To find integrant factors and phenomenon for <jats:styled-content style=\"fixed-case\">HERS<\/jats:styled-content> development and growth, we studied the proliferation and mobility of the cervical loop (<jats:styled-content style=\"fixed-case\">CL<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>We observed the cell movement of <jats:styled-content style=\"fixed-case\">CL<\/jats:styled-content> by the <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>i<jats:styled-content style=\"fixed-case\">I<\/jats:styled-content> labeling and organ culture system. To examine cell proliferation, we carried out immunostaining of <jats:styled-content style=\"fixed-case\">CL<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">HERS<\/jats:styled-content> using anti\u2010<jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>i67 antibody. Cell motility in <jats:styled-content style=\"fixed-case\">CL<\/jats:styled-content> was observed by tooth germ slice organ culture using green fluorescent protein mouse. We also examined the expression of paxillin associated with cell movement.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Imaging using <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>i<jats:styled-content style=\"fixed-case\">I<\/jats:styled-content> labeling showed that, at the apex of <jats:styled-content style=\"fixed-case\">CL<\/jats:styled-content>, the epithelium elongated in tandem with the growth of outer enamel epithelium (<jats:styled-content style=\"fixed-case\">OEE<\/jats:styled-content>). Cell proliferation assay using <jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>i67 immunostaining showed that <jats:styled-content style=\"fixed-case\">OEE<\/jats:styled-content> divided more actively than inner enamel epithelium (<jats:styled-content style=\"fixed-case\">IEE<\/jats:styled-content>) at the onset of <jats:styled-content style=\"fixed-case\">HERS<\/jats:styled-content> formation. Live imaging suggested that mobility of the <jats:styled-content style=\"fixed-case\">OEE<\/jats:styled-content> and cells in the apex of <jats:styled-content style=\"fixed-case\">CL<\/jats:styled-content> were more active than in <jats:styled-content style=\"fixed-case\">IEE<\/jats:styled-content>. The expression of paxillin was observed strongly in <jats:styled-content style=\"fixed-case\">OEE<\/jats:styled-content> and the apex of <jats:styled-content style=\"fixed-case\">CL<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The more active growth and movement of <jats:styled-content style=\"fixed-case\">OEE<\/jats:styled-content> cells contributed to <jats:styled-content style=\"fixed-case\">HERS<\/jats:styled-content> formation after reduction of the growth of <jats:styled-content style=\"fixed-case\">IEE<\/jats:styled-content>. The expression pattern of paxillin was involved in the active movement of <jats:styled-content style=\"fixed-case\">OEE<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">HERS<\/jats:styled-content>. The results will contribute to understand the <jats:styled-content style=\"fixed-case\">HERS<\/jats:styled-content> formation mechanism and elucidate the cause of anomaly root.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12003","type":"journal-article","created":{"date-parts":[[2012,9,15]],"date-time":"2012-09-15T03:15:15Z","timestamp":1347678915000},"page":"262-267","source":"Crossref","is-referenced-by-count":23,"title":["Cell dynamics in cervical loop epithelium during transition from crown to root: implications for <scp>H<\/scp>ertwig's epithelial root sheath formation"],"prefix":"10.1111","volume":"48","author":[{"given":"M.","family":"Sakano","sequence":"first","affiliation":[{"name":"Division of Developmental Biology and Regenerative Medicine Department of Anatomy Iwate Medical University Yahaba Iwate Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Otsu","sequence":"additional","affiliation":[{"name":"Division of Developmental Biology and Regenerative Medicine Department of Anatomy Iwate Medical University Yahaba Iwate Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Fujiwara","sequence":"additional","affiliation":[{"name":"Division of Developmental Biology and Regenerative Medicine Department of Anatomy Iwate Medical University Yahaba Iwate Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Fukumoto","sequence":"additional","affiliation":[{"name":"Division of Pediatric Dentistry Department of Oral Health and Development Sciences Tohoku University Graduate School of Dentistry Sendai Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Yamada","sequence":"additional","affiliation":[{"name":"Division of Pediatric Dentistry Department of Oral Health and Development Sciences Tohoku University Graduate School of Dentistry Sendai Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Harada","sequence":"additional","affiliation":[{"name":"Division of Developmental Biology and Regenerative Medicine Department of Anatomy Iwate Medical University Yahaba Iwate Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,9,14]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"85","article-title":"Short root anomaly","volume":"80","author":"Lind V","year":"1972","journal-title":"Scand J Dent Res"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2006.07.021"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-263X.1995.tb00187.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00332"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02307"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.015081"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2004.09.012"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-004-1065-5"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.b.21268"},{"key":"e_1_2_6_11_1","first-page":"79","volume-title":"Ten Cate's Oral Histology","author":"Nanci A","year":"2008"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.130.2.407"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2011.01407.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.049528"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.2330\/joralbiosci.50.154"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.033803"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.056358"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2010.11.112"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10059-011-0045-3"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.12.033"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1607"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.04.028"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-008-0655-z"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.091103"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.11.014"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.09.013"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-008-9170-7"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1202273"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1999.4566"},{"key":"e_1_2_6_30_1","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1080\/03008200290001221","article-title":"Pathways and fate of migratory cells during late tooth organogenesis","volume":"43","author":"Diekwisch TG","year":"2002","journal-title":"Connect Tissue Res"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12003","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.12003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T14:08:56Z","timestamp":1698674936000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jre.12003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,9,14]]},"references-count":29,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,4]]}},"alternative-id":["10.1111\/jre.12003"],"URL":"https:\/\/doi.org\/10.1111\/jre.12003","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,9,14]]}}}