{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T02:40:19Z","timestamp":1791081619283,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,10,22]],"date-time":"2012-10-22T00:00:00Z","timestamp":1350864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation Emphases Item of China","doi-asserted-by":"crossref","award":["30973313"],"award-info":[{"award-number":["30973313"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Matrix metalloproteinases (<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>s) play important roles in extracellular matrix degradation and may be regulated by extracellular matrix metalloproteinase inducer (<jats:styled-content style=\"fixed-case\">EMMPRIN<\/jats:styled-content>). The aim of this study was to investigate the temporal expression and localization of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20108 and <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u201013 during the development of ligature\u2010induced periodontitis in rats, and to analyze the correlations of <jats:styled-content style=\"fixed-case\">EMMPRIN<\/jats:styled-content> with <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20108 and <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u201013 in periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Periodontitis was simulated in rats by ligaturing the cervix of the lower first molars, as described in our previous method. The rats were killed 0, 3, 5, 7, 11, 15 and 21\u00a0d after ligation. Micro\u2010computed tomography examinations were performed to detect alveolar bone loss. Semiquantitative western blotting was used to assess the temporal changes in the levels of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20108, <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u201013 and <jats:styled-content style=\"fixed-case\">EMMPRIN<\/jats:styled-content> proteins in gingival tissue. Immunohistochemistry was applied to detect the expression and locations of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20108 and <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u201013 in gingival tissue and alveolar bone.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Alveolar bone loss showed an exponential increase from days 3 to 11, followed by a slower rate of loss at subsequent study time points. <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20108 showed a rapid increase of expression from baseline to a peak on day 3, a gradual decrease from days 5 to 7 and then stabilized thereafter. <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20108 was predominantly located in neutrophil\u2010like cells. Statistically, the expression of MMP\u20108 was not correlated with the expression of <jats:styled-content style=\"fixed-case\">EMMPRIN<\/jats:styled-content>. The expression of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u201013 and of <jats:styled-content style=\"fixed-case\">EMMRPIN<\/jats:styled-content> increased from days 3 to 7, and showed a moderate decrease thereafter. The immunoreactivity of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u201013 was mainly detected in monocytes\/macrophages, on the alveolar bone surface, in osteoclasts and in gingival epithelial cells. Statistically, <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u201013 had a strong, positive correlation with <jats:styled-content style=\"fixed-case\">EMMPRIN<\/jats:styled-content> (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.855, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.01).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The levels of expression of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20108 and <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u201013 are temporally varied at different periods during the development of experimental periodontitis. The level of expression of <jats:styled-content style=\"fixed-case\">EMMPRIN<\/jats:styled-content> is closely associated with the expression of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u201013, but not with the expression of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20108. In addition, <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u201013 might be involved in alveolar bone destruction, as well as in physiological bone remodeling.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12019","type":"journal-article","created":{"date-parts":[[2012,10,22]],"date-time":"2012-10-22T06:51:17Z","timestamp":1350888677000},"page":"411-419","source":"Crossref","is-referenced-by-count":20,"title":["Temporal expression of metalloproteinase\u20108 and \u201013 and their relationships with extracellular matrix metalloproteinase inducer in the development of ligature\u2010induced periodontitis in rats"],"prefix":"10.1111","volume":"48","author":[{"given":"D.","family":"Yang","sequence":"first","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of Stomatology Wuhan University Wuhan 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Hospital of Stomatology Wuhan University Wuhan China"},{"name":"Department of Periodontology School and Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Liu","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of Stomatology Wuhan University Wuhan China"},{"name":"Department of Periodontology School and Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Xiang","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of Stomatology Wuhan University Wuhan China"},{"name":"Department of Periodontology 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