{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T13:58:28Z","timestamp":1768831108947,"version":"3.49.0"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,10,28]],"date-time":"2012-10-28T00:00:00Z","timestamp":1351382400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2013,6]]},"abstract":"Background and Objectives<\/jats:title>The renin\u2013angiotensin system (RAS<\/jats:styled-content>) is considered as a hormonal circulatory system involved in maintaining blood pressure, electrolyte and fluid homeostasis. RAS<\/jats:styled-content> components can be synthesized in local tissues and are found to play a role in gingival overgrowth. The drug\u2010induced gingival overgrowth (DIGO<\/jats:styled-content>) is a fibrotic condition, which is associated with multiple factors, including inflammation and adverse drug effects such as cyclosporine A<\/jats:styled-content>. This study was directed forward to the identification of the angiotensinogen, angiotensin II<\/jats:styled-content> (A<\/jats:styled-content>ng II<\/jats:styled-content>) and its receptors AT<\/jats:styled-content>1<\/jats:sub>\/AT<\/jats:styled-content>2<\/jats:sub> expression in DIGO<\/jats:styled-content> tissues and cyclosporine\u2010treated human gingival fibroblast cells.<\/jats:p><\/jats:sec>Material and Methods<\/jats:title>Gingival samples were obtained from patients with cyclosporine\u2010induced gingival overgrowth, chronic periodontitis and normal healthy subjects. The total RNA<\/jats:styled-content> was isolated and reverse transcription\u2013polymerase chain reaction was performed for angiotensinogen, A<\/jats:styled-content>ng II<\/jats:styled-content> and AT<\/jats:styled-content>1<\/jats:sub>\/AT<\/jats:styled-content>2<\/jats:sub> receptor. A<\/jats:styled-content>ng II<\/jats:styled-content> protein was estimated from tissue by enzyme immunoassay. The expression of A<\/jats:styled-content>ng II<\/jats:styled-content> and its receptors were also examined in gingival fibroblast cells treated with cyclosporine.<\/jats:p><\/jats:sec>Results<\/jats:title>A<\/jats:styled-content>ng II<\/jats:styled-content> mRNA<\/jats:styled-content> and protein expression was significantly higher in patients with DIGO than in patients with periodontitis and healthy subjects. The AT<\/jats:styled-content>1<\/jats:sub> mRNA<\/jats:styled-content> was expressed more than AT<\/jats:styled-content>2<\/jats:sub> in all examined tissues. In gingival fibroblasts, A<\/jats:styled-content>ng II<\/jats:styled-content> and AT<\/jats:styled-content>1<\/jats:sub> expressions were increased with cyclosporine incorporation compared to controls.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>These results suggest that cyclosporine can modulate local expression of RAS<\/jats:styled-content> components such as angiotensinogen, A<\/jats:styled-content>ng II<\/jats:styled-content> and its receptors in gingival tissues and gingival fibroblast cells.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12020","type":"journal-article","created":{"date-parts":[[2012,10,29]],"date-time":"2012-10-29T17:04:33Z","timestamp":1351530273000},"page":"386-391","source":"Crossref","is-referenced-by-count":11,"title":["Retracted: Expression of angiotensin II<\/scp> and its receptors in cyclosporine\u2010induced gingival overgrowth"],"prefix":"10.1111","volume":"48","author":[{"given":"T.","family":"Subramani","sequence":"first","affiliation":[{"name":"Department of Periodontics Sri Ramachandra Dental College and Hospital Sri Ramachandra University Chennai India"},{"name":"Department of Cell and Molecular Biology Faculty of Biotechnology and Biomolecular Sciences University Putra Malaysia Serdang Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Senthilkumar","sequence":"additional","affiliation":[{"name":"Biotechnology Division National Environmental Engineering Research Institute Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Periasamy","sequence":"additional","affiliation":[{"name":"Department of Nephrology Sri Ramachandra Medical College Sri Ramachandra University Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Periodontics Sri Ramachandra Dental College and Hospital Sri Ramachandra University Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,10,28]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0895-7061(99)00103-X"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.30.4.859"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00041552-199301000-00006"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004872-199407030-00002"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajh\/5.12.221S"},{"key":"e_1_2_5_7_1","first-page":"S108","article-title":"Effect of angiotensin II on glomerular hemodynamics and ultrafiltration coefficient","volume":"20","author":"Blantz RC","year":"1987","journal-title":"Kidney Int"},{"key":"e_1_2_5_8_1","first-page":"74","article-title":"Pre\u2010glomerular structural changes in the renal vasculature in hypertension","volume":"2","author":"Anderson WP","year":"1995","journal-title":"Blood Press"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.23.10663"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(98)00448-2"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2000.027004217.x"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1834-7819.1999.tb00224.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11906-002-0067-5"},{"key":"e_1_2_5_14_1","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1681\/ASN.V1341100","article-title":"ACE inhibitors versus AT1 receptor antagonists in patients with chronic renal disease","volume":"13","author":"Hilgers KF","year":"2002","journal-title":"J Am Soc Nephrol"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/16.5.1034"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-200210000-00017"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1532-0456(02)00098-4"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2007.060172"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910508400609"},{"key":"e_1_2_5_20_1","first-page":"156","article-title":"RNA isolation by single step of acid guanidium thiocyanate\u2010phenol\u2010chloroform extraction","volume":"162","author":"Chomezynski P","year":"1985","journal-title":"Anal Biochem"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1043-2760(97)00101-X"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.80.2.219"},{"key":"e_1_2_5_23_1","first-page":"1381","article-title":"Renal extracellular matrix accumulation in acute puromycin aminonucleoside nephrosis in rats","volume":"141","author":"Jones CL","year":"1992","journal-title":"Am J Pathol"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.19.6.753"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2009.080264"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.2011.00850.x"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.1999.00481.x"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfg180"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000047090.08299.D5"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2004.12.015"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.4103\/0253-7613.66842"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1053\/ajkd.2000.9684"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0895-7061(02)02280-X"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2004.02.003"}],"updated-by":[{"DOI":"10.1111\/jre.12178","type":"retraction","label":"Retraction","source":"retraction-watch","updated":{"date-parts":[[2014,2,27]],"date-time":"2014-02-27T00:00:00Z","timestamp":1393459200000},"record-id":"4101"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12020","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12020","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.12020","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T11:45:28Z","timestamp":1698666328000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jre.12020"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,28]]},"references-count":33,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,6]]}},"alternative-id":["10.1111\/jre.12020"],"URL":"https:\/\/doi.org\/10.1111\/jre.12020","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10,28]]}}}