{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T05:00:50Z","timestamp":1792731650675,"version":"4.5.0"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,11,8]],"date-time":"2012-11-08T00:00:00Z","timestamp":1352332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2013,8]]},"abstract":"Background and Objective<\/jats:italic><\/jats:title>The aim of this study is to analyze the relationship between the subgingival colonization by Candida albicans<\/jats:italic> and other yeasts with the severity of chronic periodontitis (CP<\/jats:styled-content>).<\/jats:p><\/jats:sec>Material and Methods<\/jats:italic><\/jats:title>After sample size calculation, 40 patients with CP<\/jats:styled-content> and 20 healthy subjects (HS<\/jats:styled-content>) were included in the study. Cases of slight\u2010moderate (MCP<\/jats:styled-content>, n<\/jats:italic>\u00a0=\u00a023) and severe CP (SCP<\/jats:styled-content>, n<\/jats:italic>\u00a0=\u00a017) were defined according to the Centers for Disease Control\/American Association of Periodontology classification. Subgingival samples were acquired using sterile paper\u2010points from the sulcus or the deepest periodontal pocket of each healthy and subject with CP<\/jats:styled-content>, respectively, and were cultured aerobically on three selective media. Yeast colonies that grew on the surface of plates were later identified by biochemical reactions. Statistical tests were used to analyze the association between subgingival yeast colonization (number of yeast\u2010positive individuals and colony forming units (CFU<\/jats:styled-content>) per subject) and periodontal disease status, considering statistical significance when P<\/jats:italic>\u00a0<\u00a00.05.<\/jats:p><\/jats:sec>Results<\/jats:italic><\/jats:title>Although several yeast species were found (C. parapisilosis<\/jats:italic>, Rhodotorula<\/jats:italic> sp., C. dubliniensis<\/jats:italic> and C. tropicalis<\/jats:italic>), only C. albicans<\/jats:italic> was present in all the patients with yeast\u2010positive CP<\/jats:styled-content>. Twelve patients (30%) with CP<\/jats:styled-content> presented yeasts in the subgingival biofilm while only three patients (15%) in the HS<\/jats:styled-content> group were positive for these microorganisms. No statistical difference was found between the CP<\/jats:styled-content> and HS<\/jats:styled-content> groups (P<\/jats:italic>\u00a0=\u00a00.084). However, when the CP<\/jats:styled-content> group was divided on the basis of severity, statistical differences were observed between the SCP<\/jats:styled-content> and MCP<\/jats:styled-content> groups (47% vs. 17%, P<\/jats:italic>\u00a0=\u00a00.043), and between the SCP<\/jats:styled-content> and HS<\/jats:styled-content> groups (47% vs. 15%, P<\/jats:italic>\u00a0=\u00a00.033). No statistical difference was observed between the MCP<\/jats:styled-content> and HS<\/jats:styled-content> groups (17% vs. 15%, P<\/jats:italic>\u00a0=\u00a00.832). High densities of yeasts were found only in patients with MCP<\/jats:styled-content> and SCP<\/jats:styled-content> (mean and range 61.25 (0\u2013100) CFU<\/jats:styled-content>\/plate and mean and range 51 (0\u2013101) CFU<\/jats:styled-content>\/plate, respectively).<\/jats:p><\/jats:sec>Conclusion<\/jats:italic><\/jats:title>In this group of patients, subgingival colonization of some yeasts, especially C. albicans<\/jats:italic>, was associated with the severity of CP.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12022","type":"journal-article","created":{"date-parts":[[2012,11,9]],"date-time":"2012-11-09T05:03:23Z","timestamp":1352437403000},"page":"428-432","source":"Crossref","is-referenced-by-count":129,"title":["Association of subgingival colonization of C<\/scp>andida albicans<\/i> and other yeasts with severity of chronic periodontitis"],"prefix":"10.1111","volume":"48","author":[{"given":"A.","family":"Canabarro","sequence":"first","affiliation":[{"name":"Department of Periodontology Universidade Veiga de Almeida Rio de Janeiro Brazil"},{"name":"Department of Periodontology Universidade do Estado do Rio de Janeiro Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Valle","sequence":"additional","affiliation":[{"name":"Department of Periodontology Universidade Veiga de Almeida Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. R.","family":"Farias","sequence":"additional","affiliation":[{"name":"Department of Periodontology Universidade Veiga de Almeida Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. B.","family":"Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Mycology Instituto de Pesquisa Clinica Evandro Chagas Funda\u00e7\u00e3o Oswaldo Cruz Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Lazera","sequence":"additional","affiliation":[{"name":"Laboratory of Mycology Instituto de Pesquisa Clinica Evandro Chagas Funda\u00e7\u00e3o Oswaldo Cruz Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Wanke","sequence":"additional","affiliation":[{"name":"Laboratory of Mycology Instituto de Pesquisa Clinica Evandro Chagas Funda\u00e7\u00e3o Oswaldo Cruz Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,11,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2010.01670.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.12.2187"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1999.70.1.13"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2011.01699.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1998.tb00113.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/002203459607502S07"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.5.672"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02419.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2009.01562.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1988.tb00080.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/08959374930070020701"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(80)90147-8"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2001.028009860.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780802060899"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1354-523X.2003.00978.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1999.4.1.1"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2005.00822.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197706092962301"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.34.9.2246-2254.1996"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.4.4.633-638.2005"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9896(2000)9999:9999<::AID-PATH673>3.0.CO;2-T"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051X.1999.260303.x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2009.03.009"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.12.4520-4522.2001"},{"key":"e_1_2_6_26_1","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1128\/iai.53.2.312-316.1986","article-title":"Isolation and characteristics of collagenolytic enzyme produced by Candida albicans","volume":"53","author":"Kaminishi H","year":"1986","journal-title":"Infect Immun"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-43-4-282"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1999.tb13733.x"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.2008\/001107-0"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12022","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12022","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.12022","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T08:27:04Z","timestamp":1698654424000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jre.12022"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,11,8]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,8]]}},"alternative-id":["10.1111\/jre.12022"],"URL":"https:\/\/doi.org\/10.1111\/jre.12022","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11,8]]}}}