{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T10:30:46Z","timestamp":1749033046591},"reference-count":14,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,12,30]],"date-time":"2012-12-30T00:00:00Z","timestamp":1356825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2013,10]]},"abstract":"Background and Objective<\/jats:title>Aggregatibacter actinomycetemcomitans<\/jats:italic> is a pathogen in oral and nonoral infections. Detection and quantification of this pathogen can be performed using selective culture techniques. The aim of this study was to establish the efficacy of two known selective media in their ability to select and support the growth of A<\/jats:styled-content>. actinomycetemcomitans<\/jats:italic>.<\/jats:p><\/jats:sec>Material and Methods<\/jats:title>Trypticase soy bacitracin vancomycin (TSBV<\/jats:styled-content>) medium and brain\u2013heart infusion agar with vancomycin (D<\/jats:styled-content>entaid\u20101), as well as a modified D<\/jats:styled-content>entaid\u20101 medium (in which the brain\u2013heart infusion agar was substituted with brain\u2013heart infusion broth), were compared. Two\u2010hundred and eighteen clinical samples were used to establish the recovery rate, the number of colony\u2010forming units (CFUs) of A<\/jats:styled-content>. actinomycetemcomitans<\/jats:italic> as well as the total number of CFUs on the three different types of medium. In addition, the numbers of gram\u2010negative aerobic rods and yeasts were determined.<\/jats:p><\/jats:sec>Results<\/jats:title>Both types of D<\/jats:styled-content>entaid\u20101 medium showed a higher recovery of A<\/jats:styled-content>. actinomycetemcomitans<\/jats:italic> compared with TSBV<\/jats:styled-content>. However, these differences did not reach statistical significance. The total number of CFUs of A<\/jats:styled-content>. actinomycetemcomitans<\/jats:italic> recovered was significantly higher on D<\/jats:styled-content>entaid\u20101 compared with TSBV<\/jats:styled-content> (p<\/jats:italic>\u00a0=\u00a00.029). The mean number of gram\u2010negative aerobic rods recovered was statistically higher on both types of D<\/jats:styled-content>entaid\u20101 medium in comparison with TSBV<\/jats:styled-content>. Low numbers of yeasts were recovered occasionally on all test plates.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Dentaid\u20101 is a low\u2010cost effective alternative to TSBV<\/jats:styled-content> for the isolation and growth of A<\/jats:styled-content>. actinomycetemcomitans<\/jats:italic> from clinical samples, such as dental plaque, which contain a complex microflora.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12037","type":"journal-article","created":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T17:53:14Z","timestamp":1357062794000},"page":"549-552","source":"Crossref","is-referenced-by-count":9,"title":["Evaluation of three selective media for isolation of A<\/scp>ggregatibacter actinomycetemcomitans<\/i>"],"prefix":"10.1111","volume":"48","author":[{"given":"P.","family":"Rurenga","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology University Medical Centre Groningen Groningen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Raangs","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology University Medical Centre Groningen Groningen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Singadji","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology University Medical Centre Groningen Groningen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Wekema\u2010Mulder","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology University Medical Centre Groningen Groningen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. C. M.","family":"Veloo","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology University Medical Centre Groningen Groningen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. J.","family":"van Winkelhoff","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology University Medical Centre Groningen Groningen the Netherlands"},{"name":"Department of Dentistry and Oral Hygiene University Medical Centre Groningen Groningen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,12,30]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1999.tb00160.x"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2011.01737.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2006.00925.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1999.tb00155.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1990.tb00036.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1981.52.10.593"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.15.4.606-609.1982"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.25.10.1985-1988.1987"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.2.509-513.2001"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2010.01274.x"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2010.01643.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.24.4.638-644.1972"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.14.3.288-294.1981"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.24.4.636-638.1986"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12037","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12037","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.12037","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T14:20:37Z","timestamp":1696256437000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jre.12037"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12,30]]},"references-count":14,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2013,10]]}},"alternative-id":["10.1111\/jre.12037"],"URL":"https:\/\/doi.org\/10.1111\/jre.12037","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12,30]]}}}