{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:51:40Z","timestamp":1788871900319,"version":"build-2803163510"},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,2,27]],"date-time":"2013-02-27T00:00:00Z","timestamp":1361923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["30801298"],"award-info":[{"award-number":["30801298"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81170933"],"award-info":[{"award-number":["81170933"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["30973313"],"award-info":[{"award-number":["30973313"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2013,10]]},"abstract":"\n Background and Objective<\/jats:title>\n \n We previously demostrated that EMMPRIN participates in the periodontitis and its interaction with Cyclophilin A possibly exists in animal periodontitis models. This study is aimed to address the expression and potential role of\n c<\/jats:styled-content>\n yclophilin\n A<\/jats:styled-content>\n (\n C<\/jats:styled-content>\n yp\n A<\/jats:styled-content>\n ) in human periodontitis.\n <\/jats:p>\n <\/jats:sec>\n \n Material and Methods<\/jats:title>\n \n Gingival tissues and peripheral blood were collected from patients with moderate to severe periodontitis or from healthy donors. Western blotting and immunohistochemistry were performed to detect the expression and distribution of\n C<\/jats:styled-content>\n yp\n A<\/jats:styled-content>\n in the gingival tissues. Peripheral blood mononuclear cells (\n PBMC<\/jats:styled-content>\n s) and neutrophils were isolated from the peripheral blood by\n F<\/jats:styled-content>\n icoll\u2013\n P<\/jats:styled-content>\n aque density\u2010gradient centrifugation. Chemotaxis assays were applied to evaluate the effects of different concentrations of\n C<\/jats:styled-content>\n yp\n A<\/jats:styled-content>\n (100, 300 and 500\u00a0ng\/mL) on the migration of\n PBMC<\/jats:styled-content>\n s and neutrophils. Supernatants of human\n THP<\/jats:styled-content>\n \u20101 cells were collected after treatment with 200\u00a0ng\/mL of\n C<\/jats:styled-content>\n yp\n A<\/jats:styled-content>\n for different periods of time (1, 3, 6, 12 and 24\u00a0h) to detect the levels of interleukin (IL)\u20101\u03b2, IL\u20108 and tumor necrosis factor alpha (\n TNF<\/jats:styled-content>\n \u2010\u03b1) by\n ELISA<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Western blot analyses revealed an increase of\n C<\/jats:styled-content>\n yp\n A<\/jats:styled-content>\n expression in inflamed gingival tissues compared with healthy tissues. Immunohistochemistry identified that the over\u2010expressed\n C<\/jats:styled-content>\n yp\n A<\/jats:styled-content>\n was localized in the infiltrating cells and\/or in the extracellular matrix in the inflamed gingival connective tissues. The positive infiltrating cells contained mononuclear cells and lobulated\u2010nuclei neutrophils. Chemotactic assays showed that 300\u00a0ng\/mL of\n C<\/jats:styled-content>\n yp\n A<\/jats:styled-content>\n apparently facilitated the chemotaxis of\n PBMC<\/jats:styled-content>\n s\/neutrophils from healthy donors, compared with the no\u2010treatment control (\n p<\/jats:italic>\n \u00a0<\u00a00.01 for\n PBMC<\/jats:styled-content>\n s,\n p<\/jats:italic>\n \u00a0<\u00a00.05 for neutrophils), whereas 100 and 500\u00a0ng\/mL of\n C<\/jats:styled-content>\n yp\n A<\/jats:styled-content>\n only weakly enhanced the chemotaxis of\n PBMC<\/jats:styled-content>\n s\/neutrophils (\n p<\/jats:italic>\n \u00a0>\u00a00.05 for\n PBMC<\/jats:styled-content>\n s\/neutrophils, not significant). The PBMCs\/neutrophils from patients with periodontitis exhibited a stronger ability to migrate when stimulated with 300\u00a0ng\/mL of\n C<\/jats:styled-content>\n yp\n A<\/jats:styled-content>\n than did\n PBMC<\/jats:styled-content>\n s\/neutrophils from healthy donors (\n p<\/jats:italic>\n \u00a0<\u00a00.05 for\n PBMC<\/jats:styled-content>\n s,\n p<\/jats:italic>\n \u00a0<\u00a00.01 for neutrophils).\n ELISA<\/jats:styled-content>\n revealed that the level of\n TNF<\/jats:styled-content>\n \u2010\u03b1 secreted by THP\u20101 cells was elevated after treatment with 200\u00a0ng\/\n mL<\/jats:styled-content>\n of\n C<\/jats:styled-content>\n yp\n A<\/jats:styled-content>\n for 12\u00a0h compared with the no\u2010treatment 0\u2010h control (\n p<\/jats:italic>\n \u00a0<\u00a00.05). The IL\u20108 level was sharply raised after 3\u00a0h of stimulation with 200\u00a0ng\/mL of\n C<\/jats:styled-content>\n yp\n A<\/jats:styled-content>\n (\n p<\/jats:italic>\n \u00a0<\u00a00.01 compared with 0\u00a0h), but no significant change was observed at the other time points (\n p<\/jats:italic>\n \u00a0>\u00a00.05). There was no statistical difference at any of the treatment time points for the secretion of IL\u20101\u03b2 (\n p<\/jats:italic>\n \u00a0>\u00a00.05 for 1, 3, 6, 12 and 24\u00a0h compared with 0\u00a0h).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n CypA participates in the pathogenesis of human periodontitis. It may be involved in the inflammatory response of periodontal tissues through inducing the chemotaxis of\n PBMC<\/jats:styled-content>\n s\/neutrophils and the secretion of\n TNF<\/jats:styled-content>\n \u2010\u03b1\/\n IL<\/jats:styled-content>\n \u20108.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jre.12047","type":"journal-article","created":{"date-parts":[[2013,2,27]],"date-time":"2013-02-27T06:39:26Z","timestamp":1361947166000},"page":"615-622","source":"Crossref","is-referenced-by-count":19,"title":["Over\u2010expression and potential role of cyclophilin A in human periodontitis"],"prefix":"10.1111","volume":"48","author":[{"given":"L.","family":"Liu","sequence":"first","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST KLOS) Key Laboratory for Oral Biomedical Engineering of Ministry of Education (KLOBME) School & Hospital of Stomatology Wuhan University Wuhan 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