{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T04:28:28Z","timestamp":1789187308722,"version":"build-2803163510"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,2,27]],"date-time":"2013-02-27T00:00:00Z","timestamp":1361923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Higher Education Commission of Pakistan"},{"name":"Academic Centre for Dentistry Amsterdam"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2013,10]]},"abstract":"Background and Objective<\/jats:title>To assess inflammatory reactions of fibroblasts in the pathophysiology of peri\u2010implantitis, we compared the pro\u2010inflammatory and matrix\u2010degrading responses of gingival and granulation tissue fibroblasts from periodontally healthy controls, peri\u2010implantitis, and periodontitis lesions to an in vitro<\/jats:italic> challenge with P<\/jats:styled-content>orphyromonas gingivalis<\/jats:italic>.<\/jats:p><\/jats:sec>Methods<\/jats:title>Fibroblasts from periodontally healthy, peri\u2010implantitis and periodontitis donors were challenged with viable P<\/jats:styled-content>.\u00a0gingivalis<\/jats:italic>. The inflammatory reactions of fibroblasts were analyzed before and after 6\u00a0h P<\/jats:styled-content>.\u00a0gingivalis<\/jats:italic> challenge, and 2.5 and 18\u00a0h after removal of the challenge. Gene expression and induction of pro\u2010inflammatory mediators, and matrix metalloproteinases (MMP<\/jats:styled-content>s) were assessed by real\u2010time polymerase chain reaction. Protein expression was measured by enzyme\u2010linked immunosorbent assay.<\/jats:p><\/jats:sec>Results<\/jats:title>Non\u2010challenged fibroblasts from peri\u2010implantitis and periodontitis lesions expressed higher levels of interleukin (IL<\/jats:styled-content>)\u20101\u03b2<\/jats:italic>, IL<\/jats:styled-content>\u20108<\/jats:italic>, and monocyte chemotactic protein (MCP<\/jats:styled-content>)\u20101<\/jats:italic> than fibroblasts from periodontally healthy individuals. The P<\/jats:styled-content>.\u00a0gingivalis<\/jats:italic> challenge induced expression of IL<\/jats:styled-content>\u20101\u03b2<\/jats:italic>, IL<\/jats:styled-content>\u20108<\/jats:italic>, IL<\/jats:styled-content>\u20106<\/jats:italic>, MCP<\/jats:styled-content>\u20101<\/jats:italic>, and MMP<\/jats:styled-content>\u20101<\/jats:italic> in periodontitis and peri\u2010implantitis fibroblasts, but not in fibroblasts from periodontally healthy individuals. MMP<\/jats:styled-content>\u20108<\/jats:italic> expression was higher in non\u2010challenged peri\u2010implantitis fibroblasts than in fibroblasts from periodontally healthy individuals. However, the P<\/jats:styled-content>.\u00a0gingivalis<\/jats:italic> challenge downregulated MMP<\/jats:styled-content>\u20108<\/jats:italic> gene expression in peri\u2010implantitis fibroblasts. After removal of the P<\/jats:styled-content>.\u00a0gingivalis<\/jats:italic> challenge, peri\u2010implantitis fibroblasts sustained higher induction of IL<\/jats:styled-content>\u20101\u03b2, MCP<\/jats:styled-content>\u20101<\/jats:italic>, and MMP<\/jats:styled-content>\u20101<\/jats:italic> compared to periodontitis fibroblasts.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Fibroblasts from peri\u2010implantitis and periodontitis lesions gave a more pronounced inflammatory response to the P<\/jats:styled-content>.\u00a0gingivalis<\/jats:italic> challenge than fibroblasts from healthy donors. They may therefore be involved in the development of inflammation in peri\u2010implantitis and periodontitis. Moreover, the sustained upregulation of inflammatory mediators and MMP<\/jats:styled-content>\u20101<\/jats:italic> in peri\u2010implantitis fibroblasts may play a role in the pathogenesis of peri\u2010implantitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12051","type":"journal-article","created":{"date-parts":[[2013,2,27]],"date-time":"2013-02-27T11:41:00Z","timestamp":1361965260000},"page":"647-656","source":"Crossref","is-referenced-by-count":27,"title":["Cytokine and matrix metalloproteinase expression in fibroblasts from peri\u2010implantitis lesions in response to viable P<\/scp>orphyromonas gingivalis<\/i>"],"prefix":"10.1111","volume":"48","author":[{"given":"M.","family":"Irshad","sequence":"first","affiliation":[{"name":"Department of Preventive Dentistry Academic Centre for Dentistry Amsterdam (ACTA) University of Amsterdam and VU University Amsterdam Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Scheres","sequence":"additional","affiliation":[{"name":"Department of Preventive Dentistry Academic Centre for Dentistry Amsterdam (ACTA) University of Amsterdam and VU University Amsterdam Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Anssari Moin","sequence":"additional","affiliation":[{"name":"Department of Periodontology ACTA University of Amsterdam and VU University Amsterdam Amsterdam The Netherlands"},{"name":"Department of Oral Function and Restorative Dentistry Section of Oral Implantology and Prosthodontics Research Institute MOVE, ACTA University of Amsterdam and VU University Amsterdam Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Crielaard","sequence":"additional","affiliation":[{"name":"Department of Preventive Dentistry Academic Centre for Dentistry Amsterdam (ACTA) University of Amsterdam and VU University Amsterdam Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. 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