{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T14:45:18Z","timestamp":1768488318099,"version":"3.49.0"},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,2,27]],"date-time":"2013-02-27T00:00:00Z","timestamp":1361923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"FISH analysis"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p><jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>ynergistetes<\/jats:italic> is a novel bacterial phylum consisting of gram\u2010negative anaerobes. Increasing lines of evidence demonstrate that this phylum is associated with periodontal diseases. This study aimed to compare the presence and levels of <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>ynergistetes<\/jats:italic> clusters <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>, in saliva of patients with chronic periodontitis (<jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content>), generalized aggressive periodontitis (<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>) and non\u2010periodontitis subjects, and investigate their correlation with clinical parameters.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Saliva was collected from patients with <jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content> (<jats:italic>n<\/jats:italic>\u00a0=\u00a020), <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content> (<jats:italic>n<\/jats:italic>\u00a0=\u00a021) and non\u2010periodontitis subjects (<jats:italic>n<\/jats:italic>\u00a0=\u00a018). Full mouth clinical periodontal measurements were recorded. The numbers of <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>ynergistetes<\/jats:italic> cluster <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content> and cluster <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> or the associated species <jats:italic><jats:styled-content style=\"fixed-case\">J<\/jats:styled-content>onquetella anthropi<\/jats:italic> were quantified by fluorescent <jats:italic>in situ<\/jats:italic> hybridization and microscopy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>ynergistetes<\/jats:italic> cluster <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content> bacteria were detected more frequently, and at higher numbers and proportions in the two periodontitis groups, than the non\u2010periodontitis control group. The prevalence was 27.7% in the control group, 85% in <jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content> and 86% in <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>. Compared to the control group, the numbers were significantly higher by 12.5\u2010fold in <jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content> and 26.5\u2010fold in <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>, whereas the difference between the two forms of periodontitis was not statistically significant. Within the total bacterial population, the proportion of this cluster was increased in <jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content> compared to the control group, with the difference between the two forms of periodontitis being also significant. There was a positive correlation between the levels of <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>ynergistetes<\/jats:italic> cluster <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content> in saliva and all full mouth clinical periodontal parameters. Nevertheless, <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>ynergistetes<\/jats:italic> cluster <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> bacteria and <jats:italic><jats:styled-content style=\"fixed-case\">J<\/jats:styled-content>.\u00a0anthropi<\/jats:italic> species were detected infrequently and at low levels in all the three subject groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>ynergistetes<\/jats:italic> cluster <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>, but not cluster <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>, bacteria are found at higher prevalence, numbers and proportions in saliva from patients with periodontitis, than non\u2010periodontitis subjects. These findings support the association of this cluster with periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12061","type":"journal-article","created":{"date-parts":[[2013,2,27]],"date-time":"2013-02-27T11:37:28Z","timestamp":1361965048000},"page":"727-732","source":"Crossref","is-referenced-by-count":28,"title":["<i><scp>S<\/scp>ynergistetes<\/i> cluster <scp>A<\/scp> in saliva is associated with periodontitis"],"prefix":"10.1111","volume":"48","author":[{"given":"G. 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