{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T07:52:45Z","timestamp":1740124365595,"version":"3.37.3"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,3,25]],"date-time":"2013-03-25T00:00:00Z","timestamp":1364169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation","doi-asserted-by":"crossref","award":["81170962"],"award-info":[{"award-number":["81170962"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Projects of Science and Technology Department of Jiangsu Province","award":["BK2011763","BK2008363"],"award-info":[{"award-number":["BK2011763","BK2008363"]}]},{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"crossref","award":["PAPD2011-2013"],"award-info":[{"award-number":["PAPD2011-2013"]}],"id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Keratinocyte growth factor (<jats:styled-content style=\"fixed-case\">KGF<\/jats:styled-content>) and its receptor (<jats:styled-content style=\"fixed-case\">KGFR<\/jats:styled-content>) are involved in hyperplastic diseases. This study explored the effect of intercellular communication on <jats:styled-content style=\"fixed-case\">KGF<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">KGFR<\/jats:styled-content> in cocultured\/monocultured gingival fibroblasts and keratinocytes following treatment with nifedipine.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Human gingival fibroblasts and keratinocytes were monocultured and cocultured, respectively. <jats:styled-content style=\"fixed-case\">MTT<\/jats:styled-content> was used to investigate the effects of nifedipine on the proliferation of gingival fibroblasts and keratinocytes. Monoculture and coculture systems were treated with different concentrations (0, 0.2 or 20\u00a0\u03bcg\/mL) of nifedipine, and the expression of <jats:italic><jats:styled-content style=\"fixed-case\">KGF<\/jats:styled-content><\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">KGFR<\/jats:styled-content><\/jats:italic> m<jats:styled-content style=\"fixed-case\">RNAs<\/jats:styled-content> was examined by <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, whilst the secretion of KGF and the expression of <jats:styled-content style=\"fixed-case\">KGFR<\/jats:styled-content> on the membrane were analyzed using ELISA and flow cytometry, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Nifedipine (0, 0.2 and 20\u00a0\u03bcg\/mL) had no influence on cell proliferation within 3\u00a0d. <jats:italic>KGF<\/jats:italic> and <jats:italic>KGFR<\/jats:italic> m<jats:styled-content style=\"fixed-case\">RNAs<\/jats:styled-content> were up\u2010regulated, but only in the cocultures. In coculture, the secretion of KGF was significantly increased by nifedipine, while it was only significantly up\u2010regulated by 20\u00a0\u03bcg\/<jats:styled-content style=\"fixed-case\">mL<\/jats:styled-content> of nifedipine in monoculture. Moreover, the level of <jats:styled-content style=\"fixed-case\">KGFR<\/jats:styled-content> protein in the membrane was significantly increased by 20\u00a0\u03bcg\/mL of nifedipine in monocultures, while it was significantly down\u2010regulated by 20\u00a0\u03bcg\/mL of nifedipine in cocultures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The expression of <jats:styled-content style=\"fixed-case\">KGF<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">KGFR<\/jats:styled-content> are influenced by the interplay of gingival keratinocytes and fibroblasts. Epithelial keratinocytes and mesenchymal fibroblasts may interplay to dynamically regulate gene expression, which may have an effect on the gingival condition following treatment with nifedipine.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12064","type":"journal-article","created":{"date-parts":[[2013,3,26]],"date-time":"2013-03-26T02:14:50Z","timestamp":1364264090000},"page":"740-747","source":"Crossref","is-referenced-by-count":5,"title":["Effect of nifedipine on the expression of keratinocyte growth factor and its receptor in cocultured\/monocultured fibroblasts and keratinocytes"],"prefix":"10.1111","volume":"48","author":[{"given":"C\u2010P.","family":"Di","sequence":"first","affiliation":[{"name":"Institute of Stomatology, Nanjing Medical University Nanjing China"},{"name":"Department of Periodontology College of Stomatology, Nanjing Medical University Nanjing 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