{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T02:00:48Z","timestamp":1792029648048,"version":"4.4.0"},"reference-count":74,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,5,28]],"date-time":"2013-05-28T00:00:00Z","timestamp":1369699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007469","name":"Higher Education Research Promotion","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007469","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Research University Project of Thailand","award":["AS549A"],"award-info":[{"award-number":["AS549A"]}]},{"name":"Thailand Government Research Fund"},{"name":"Developing Research Unit in Herbal Medicine for Oral Tissue Regeneration Fund"},{"name":"90th Anniversary of Chulalongkorn University Fund"},{"name":"Ratchadaphiseksomphot Endowment Fund"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Periodontal disease is a common infectious disease, found worldwide, causing the destruction of the periodontium. The periodontium is a complex structure composed of both soft and hard tissues, thus an agent applied to regenerate the periodontium must be able to stimulate periodontal ligament, cementum and alveolar bone regeneration. Recent studies demonstrated that acemannan, a polysaccharide extracted from <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>loe vera<\/jats:italic> gel, stimulated both soft and hard tissue healing. This study investigated effect of acemannan as a bioactive molecule and scaffold for periodontal tissue regeneration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Primary human periodontal ligament cells were treated with acemannan <jats:italic>in vitro<\/jats:italic>. New <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> synthesis, expression of  growth\/differentiation factor 5 and runt\u2010related transcription factor 2, expression of vascular endothelial growth factor, bone morphogenetic protein\u20102 and type I collagen, alkaline phosphatase activity, and mineralized nodule formation were determined using [<jats:sup>3<\/jats:sup>H]\u2010thymidine incorporation, reverse transcription\u2013polymerase chain reaction, enzyme\u2010linked immunoabsorbent assay, biochemical assay and alizarin red staining, respectively. In our <jats:italic>in vivo<\/jats:italic> study, premolar class <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> furcation defects were made in four mongrel dogs. Acemannan sponges were applied into the defects. Untreated defects were used as a negative control group. The amount of new bone, cementum and periodontal ligament formation were evaluated 30 and 60\u00a0d after the operation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Acemannan significantly increased periodontal ligament cell proliferation, upregulation of <jats:styled-content style=\"fixed-case\">growth\/differentiation factor 5<\/jats:styled-content>, runt\u2010related transcription factor 2, <jats:styled-content style=\"fixed-case\">vascular endothelial growth factor<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> bone morphogenetic protein 2<\/jats:styled-content>, type I collagen and alkaline phosphatase activity, and mineral deposition as compared with the untreated control group <jats:italic>in vitro<\/jats:italic>. Moreover, acemannan significantly accelerated new alveolar bone, cementum and periodontal ligament formation in class <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> furcation defects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our data suggest that acemannan could be a candidate biomolecule for periodontal tissue regeneration.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12090","type":"journal-article","created":{"date-parts":[[2013,5,28]],"date-time":"2013-05-28T09:08:57Z","timestamp":1369732137000},"page":"164-178","source":"Crossref","is-referenced-by-count":97,"title":["Acemannan sponges stimulate alveolar bone, cementum and periodontal ligament regeneration in a canine class <scp>II<\/scp> furcation defect model"],"prefix":"10.1111","volume":"49","author":[{"given":"P.","family":"Chantarawaratit","sequence":"first","affiliation":[{"name":"Faculty of Dentistry Dental Biomaterials Program Graduate School Chulalongkorn University Bangkok Thailand"},{"name":"Department of Materials Science Faculty of Science Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Sangvanich","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Science Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Banlunara","sequence":"additional","affiliation":[{"name":"Department of <i>Pathology<\/i> Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Soontornvipart","sequence":"additional","affiliation":[{"name":"Department of Surgery Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Thunyakitpisal","sequence":"additional","affiliation":[{"name":"Research Unit of Herbal Medicine and Natural Product for Dental Application Department of Anatomy Faculty of Dentistry Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,5,28]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"77","article-title":"Nonsurgical periodontal therapy","volume":"2001","author":"Drisko CH","year":"2000","journal-title":"Periodontol"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1263\/jbb.103.1"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/154411130201300605"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.4103\/0970-9290.74232"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/10_2010_93"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm101110x"},{"key":"e_1_2_7_8_1","first-page":"77","article-title":"Alginates in tissue engineering","volume":"238","author":"Wong M","year":"2004","journal-title":"Methods Mol Biol"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmret.2005.04.006"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.1519"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10266\u2010012\u20100101\u20102"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1254\/jphs.08204FP"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2009.0593"},{"key":"e_1_2_7_14_1","first-page":"4069","article-title":"Acemannan induces cementoblast proliferation, differentiation, extracellular matrix secretion, and mineral deposition","volume":"6","author":"Sahawat D","year":"2012","journal-title":"J Med Plant Res"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/acm.2012.0164"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2004.07.006"},{"key":"e_1_2_7_17_1","first-page":"149","article-title":"Effect of acemannan on BMP\u20102 expression in primary pulpal fibroblasts and periodontal fibroblasts, in vitro study","volume":"4","author":"Jittapiromsak N","year":"2007","journal-title":"J Oral Tissue Eng"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carres.2005.02.016"},{"issue":"7","key":"e_1_2_7_19_1","first-page":"167","article-title":"Differentiation potential of stem cells from human dental origin \u2010 promise for tissue engineering","volume":"60","author":"Kadar K","year":"2009","journal-title":"J Physiol Pharmacol"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(76)90060-1"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1254\/jjp.84.146"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-4931(02)00016-4"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200203014"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-007-3032-7"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2011.06.020"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.4248\/IJOS10056"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2008.06.004"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(83)90303-4"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016357.2012.680909"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.050209"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2012.01475.x"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2011.03.003"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-6343.2007.00399.x"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16627-0"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(00)00562-1"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80257-3"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345990780100701"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2010.01281.x"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2002.17.8.1441"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2010.01576.x"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2009.01527.x"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2003.00695.x"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345970760080801"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2008.04.023"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-7254.2007.00586.x"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748-6041\/4\/6\/065010"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.22085"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345920710020801"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20109"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2003.07.002"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2008.070327"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1177\/0883911510383684"},{"key":"e_1_2_7_53_1","first-page":"356","volume-title":"Proceeding of International Conference: Innovative Research in a Changing and Challenging World","author":"Boonpaisanseree W","year":"2012"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22829"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M513277200"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0512231"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1987.tb01506.x"},{"key":"e_1_2_7_58_1","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/S0011-8532(22)00858-8","article-title":"Current status of periodontal therapy for furcation involvements","volume":"35","author":"Carranza FA","year":"1991","journal-title":"Dent Clin North Am"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1992.tb00632.x"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-9657.1985.tb00555.x"},{"key":"e_1_2_7_61_1","first-page":"21","article-title":"Periodontal wound healing and regeneration","volume":"1999","author":"Wikesjo UM","year":"2000","journal-title":"Periodontol"},{"key":"e_1_2_7_62_1","first-page":"651","article-title":"Dental cementum reviewed: development, structure, composition, regeneration and potential functions","volume":"4","author":"Goncalves PF","year":"2005","journal-title":"Braz J Oral Sci"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2008.01158.x"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2005.00794.x"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2004.00541.x"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.4248\/IJOS11009"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2005.00785.x"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.5051\/jpis.2010.40.1.3"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1972.tb00633.x"},{"key":"e_1_2_7_70_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-005-1106-8"},{"key":"e_1_2_7_71_1","doi-asserted-by":"publisher","DOI":"10.2220\/biomedres.31.319"},{"key":"e_1_2_7_72_1","doi-asserted-by":"publisher","DOI":"10.1517\/17425247.3.5.647"},{"key":"e_1_2_7_73_1","first-page":"CD001724","article-title":"Guided tissue regeneration for periodontal infra\u2010bony defects","volume":"19","author":"Needleman IG","year":"2006","journal-title":"Cochrane Database Syst Rev"},{"key":"e_1_2_7_74_1","first-page":"171","article-title":"Human histologic and clinical evaluation of recombinant human platelet\u2010derived growth factor and beta\u2010tricalcium phosphate for the treatment of periodontal intraosseous defects","volume":"28","author":"Ridgway HK","year":"2008","journal-title":"Int J Periodontics Restorative Dent"},{"key":"e_1_2_7_75_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.12.2205"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12090","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12090","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.12090","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T00:42:19Z","timestamp":1696207339000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jre.12090"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5,28]]},"references-count":74,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,4]]}},"alternative-id":["10.1111\/jre.12090"],"URL":"https:\/\/doi.org\/10.1111\/jre.12090","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5,28]]}}}